Advanced search

Novel primer sequences for polymerase chain reaction-based detection of Trypanosoma brucei gambiense.

Author
Organization
Abstract
Progress in diagnosis, treatment, and epidemiology of human African trypanosomiasis (sleeping sickness) depends on the existence of specific and sensitive diagnostic tools. Inherent shortcomings of serologic and parasitologic diagnostic methods can be overcome by molecular techniques. Therefore. we have developed a new polymerase chain reaction (PCR) test using primers derived from the recently identified sequence of the Trypanosoma brucei gambiense-specific glycoprotein (TgsGP). The specificity of the TgsGP-PCR was evaluated on DNA extracted from 73 different trypanosome populations belonging to diverse taxonomic groups that were isolated from various host species, and from different geographic origins. The TgsG-PCR was shown to be specific for T. b. gambiense and was suitable for detection of trypanosome DNA in blood samples of patients with confirmed sleeping sickness.
Keywords
SLEEPING-SICKNESS SUSPECTS, SURFACE GLYCOPROTEIN GENES, NORTH-WESTERN UGANDA, HUMAN-SERUM, AFRICAN TRYPANOSOMIASIS, PCR AMPLIFICATION, ARBITRARY PRIMERS, ANIMAL RESERVOIR, IDENTIFICATION, EXPRESSION

Citation

Please use this url to cite or link to this publication:

Chicago
Radwanska, Magdalena, Filip Claes, Stefan Magez, Eddy Magnus, David Perez-Morga, Etienne Pays, and Philippe Büscher. 2002. “Novel Primer Sequences for Polymerase Chain Reaction-based Detection of Trypanosoma Brucei Gambiense.” American Journal of Tropical Medicine and Hygiene 67 (3): 289–295.
APA
Radwanska, M., Claes, F., Magez, S., Magnus, E., Perez-Morga, D., Pays, E., & Büscher, P. (2002). Novel primer sequences for polymerase chain reaction-based detection of Trypanosoma brucei gambiense. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 67(3), 289–295.
Vancouver
1.
Radwanska M, Claes F, Magez S, Magnus E, Perez-Morga D, Pays E, et al. Novel primer sequences for polymerase chain reaction-based detection of Trypanosoma brucei gambiense. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE. 2002;67(3):289–95.
MLA
Radwanska, Magdalena et al. “Novel Primer Sequences for Polymerase Chain Reaction-based Detection of Trypanosoma Brucei Gambiense.” AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE 67.3 (2002): 289–295. Print.
@article{8572074,
  abstract     = {Progress in diagnosis, treatment, and epidemiology of human African trypanosomiasis (sleeping sickness) depends on the existence of specific and sensitive diagnostic tools. Inherent shortcomings of serologic and parasitologic diagnostic methods can be overcome by molecular techniques. Therefore. we have developed a new polymerase chain reaction (PCR) test using primers derived from the recently identified sequence of the Trypanosoma brucei gambiense-specific glycoprotein (TgsGP). The specificity of the TgsGP-PCR was evaluated on DNA extracted from 73 different trypanosome populations belonging to diverse taxonomic groups that were isolated from various host species, and from different geographic origins. The TgsG-PCR was shown to be specific for T. b. gambiense and was suitable for detection of trypanosome DNA in blood samples of patients with confirmed sleeping sickness.},
  author       = {Radwanska, Magdalena and Claes, Filip and Magez, Stefan and Magnus, Eddy and Perez-Morga, David and Pays, Etienne and B{\"u}scher, Philippe},
  issn         = {0002-9637},
  journal      = {AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE},
  language     = {eng},
  number       = {3},
  pages        = {289--295},
  title        = {Novel primer sequences for polymerase chain reaction-based detection of Trypanosoma brucei gambiense.},
  url          = {http://dx.doi.org/10.4269/ajtmh.2002.67.289},
  volume       = {67},
  year         = {2002},
}

Altmetric
View in Altmetric
Web of Science
Times cited: