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Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes

(2002) JOURNAL OF CLINICAL MICROBIOLOGY. 40(11). p.4295-4297
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Abstract
We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes were designed to target subgenus-specific sequences on the multiple-copy 18S rRNA, greatly facilitating the detection of a single trypanosome.
Keywords
SLEEPING-SICKNESS SUSPECTS, POLYMERASE-CHAIN-REACTION, BRUCEI-GAMBIENSE, PCR AMPLIFICATION, EXPRESSION, DIAGNOSIS, CAMEROON, UGANDA, CATT

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Chicago
Radwanska, Magdalena, Stefan Magez, H Perry-O’Keefe, H Stender, J Coull, JM Sternberg, P Buscher, and JJ Hyldig-Nielsen. 2002. “Direct Detection and Identification of African Trypanosomes by Fluorescence in Situ Hybridization with Peptide Nucleic Acid Probes.” Journal of Clinical Microbiology 40 (11): 4295–4297.
APA
Radwanska, M., Magez, S., Perry-O’Keefe, H., Stender, H., Coull, J., Sternberg, J., Buscher, P., et al. (2002). Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes. JOURNAL OF CLINICAL MICROBIOLOGY, 40(11), 4295–4297.
Vancouver
1.
Radwanska M, Magez S, Perry-O’Keefe H, Stender H, Coull J, Sternberg J, et al. Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes. JOURNAL OF CLINICAL MICROBIOLOGY. 2002;40(11):4295–7.
MLA
Radwanska, Magdalena et al. “Direct Detection and Identification of African Trypanosomes by Fluorescence in Situ Hybridization with Peptide Nucleic Acid Probes.” JOURNAL OF CLINICAL MICROBIOLOGY 40.11 (2002): 4295–4297. Print.
@article{8572073,
  abstract     = {We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes were designed to target subgenus-specific sequences on the multiple-copy 18S rRNA, greatly facilitating the detection of a single trypanosome.},
  author       = {Radwanska, Magdalena and Magez, Stefan and Perry-O'Keefe, H and Stender, H and Coull, J and Sternberg, JM and Buscher, P and Hyldig-Nielsen, JJ},
  issn         = {0095-1137},
  journal      = {JOURNAL OF CLINICAL MICROBIOLOGY},
  language     = {eng},
  number       = {11},
  pages        = {4295--4297},
  title        = {Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes},
  url          = {http://dx.doi.org/10.1128/jcm.40.11.4295-4297.2002},
  volume       = {40},
  year         = {2002},
}

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