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The serum resistance-associated gene as a diagnostic tool for the detection of Trypanosoma brucei rhodesiense

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Abstract
In the search for new diagnostic methods that would distinguish Trypanosoma brucei rhodesiense from T. b. brucei and T b. gambiense, we have developed two polymerase chain reaction (PCR) primer sets. The first primer set was derived from the serum resistance-associated (SRA) gene of T. b. rhodesiense that confers resistance to lysis by normal human serum (NHS). The specificity of the SRA-based PCR was tested on 97 different trypanosome populations originating from various taxonomic groups, host species, and geographic regions. Only one of 25 T b. rhodesiense samples was negative in this PCR. and none of 72 other samples were positive in this assay. Interestingly, a reference T. brucei strain (TREU927/4) currently used for genome sequencing was negative for the SRA gene; however, this strain was resistant to lysis by NHS. The second primer set was derived from a specific variant surface glycoprotein (VSG) expression site where the SRA gene is expressed (R-ES). This primer set identified the strain as T. b. rhodesiense in 17 of 17 SRA gene-positive strains in which it was tested. These data strongly suggest that expression of the SRA gene is generally involved in resistance to lysis by NHS in T. b. rhodesiense strains.
Keywords
SURFACE GLYCOPROTEIN GENES, SLEEPING SICKNESS, ANIMAL RESERVOIR, ANTIGEN GENE, GAMBIENSE, STOCKS, EXPRESSION, IDENTIFICATION, UGANDA, CONGO

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Chicago
Radwanska, Magdalena, Mustapha Chamekh, Luc Vanhamme, Filip Claes, Stefan Magez, Eddy Magnus, Patrick de Baetselier, Philippe Büscher, and Etienne Pays. 2002. “The Serum Resistance-associated Gene as a Diagnostic Tool for the Detection of Trypanosoma Brucei Rhodesiense.” American Journal of Tropical Medicine and Hygiene 67 (6): 684–690.
APA
Radwanska, M., Chamekh, M., Vanhamme, L., Claes, F., Magez, S., Magnus, E., de Baetselier, P., et al. (2002). The serum resistance-associated gene as a diagnostic tool for the detection of Trypanosoma brucei rhodesiense. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 67(6), 684–690.
Vancouver
1.
Radwanska M, Chamekh M, Vanhamme L, Claes F, Magez S, Magnus E, et al. The serum resistance-associated gene as a diagnostic tool for the detection of Trypanosoma brucei rhodesiense. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE. 2002;67(6):684–90.
MLA
Radwanska, Magdalena et al. “The Serum Resistance-associated Gene as a Diagnostic Tool for the Detection of Trypanosoma Brucei Rhodesiense.” AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE 67.6 (2002): 684–690. Print.
@article{8572072,
  abstract     = {In the search for new diagnostic methods that would distinguish Trypanosoma brucei rhodesiense from T. b. brucei and T b. gambiense, we have developed two polymerase chain reaction (PCR) primer sets. The first primer set was derived from the serum resistance-associated (SRA) gene of T. b. rhodesiense that confers resistance to lysis by normal human serum (NHS). The specificity of the SRA-based PCR was tested on 97 different trypanosome populations originating from various taxonomic groups, host species, and geographic regions. Only one of 25 T b. rhodesiense samples was negative in this PCR. and none of 72 other samples were positive in this assay. Interestingly, a reference T. brucei strain (TREU927/4) currently used for genome sequencing was negative for the SRA gene; however, this strain was resistant to lysis by NHS. The second primer set was derived from a specific variant surface glycoprotein (VSG) expression site where the SRA gene is expressed (R-ES). This primer set identified the strain as T. b. rhodesiense in 17 of 17 SRA gene-positive strains in which it was tested. These data strongly suggest that expression of the SRA gene is generally involved in resistance to lysis by NHS in T. b. rhodesiense strains.},
  author       = {Radwanska, Magdalena and Chamekh, Mustapha and Vanhamme, Luc and Claes, Filip and Magez, Stefan and Magnus, Eddy and de Baetselier, Patrick and B{\"u}scher, Philippe and Pays, Etienne},
  issn         = {0002-9637},
  journal      = {AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE},
  language     = {eng},
  number       = {6},
  pages        = {684--690},
  title        = {The serum resistance-associated gene as a diagnostic tool for the detection of Trypanosoma brucei rhodesiense},
  url          = {http://dx.doi.org/10.4269/ajtmh.2002.67.684},
  volume       = {67},
  year         = {2002},
}

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