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Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy

Jing Liu, Ranhua Xiong (UGent) , Toon Brans (UGent) , Saskia Lippens (UGent) , Eef Parthoens (UGent) , Francesca Cella Zanacchi, Raffaella Magrassi, Santosh K Singh, Sreekumar Kurungot, Sabine Szunerits, et al.
(2018) LIGHT-SCIENCE & APPLICATIONS. 7.
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Abstract
In the replacement of genetic probes, there is increasing interest in labeling living cells with high-quality extrinsic labels, which avoid over-expression artifacts and are available in a wide spectral range. This calls for a broadly applicable technology that can deliver such labels unambiguously to the cytosol of living cells. Here, we demonstrate that nanoparticle-sensitized photoporation can be used to this end as an emerging intracellular delivery technique. We replace the traditionally used gold nanoparticles with graphene nanoparticles as photothermal sensitizers to permeabilize the cell membrane upon laser irradiation. We demonstrate that the enhanced thermal stability of graphene quantum dots allows the formation of multiple vapor nanobubbles upon irradiation with short laser pulses, allowing the delivery of a variety of extrinsic cell labels efficiently and homogeneously into live cells. We demonstrate high-quality time-lapse imaging with confocal, total internal reflection fluorescence (TIRF), and Airyscan super-resolution microscopy. As the entire procedure is readily compatible with fluorescence (super resolution) microscopy, photoporation with graphene quantum dots has the potential to become the long-awaited generic platform for controlled intracellular delivery of fluorescent labels for live-cell imaging.
Keywords
TAG FUSION PROTEINS, INTRACELLULAR DELIVERY, GOLD NANOPARTICLES, LIVING CELLS, MAMMALIAN-CELLS, NANOMATERIALS, TRAFFICKING, NANOBUBBLES, NANOBODIES, MOLECULES

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MLA
Liu, Jing, et al. “Repeated Photoporation with Graphene Quantum Dots Enables Homogeneous Labeling of Live Cells with Extrinsic Markers for Fluorescence Microscopy.” LIGHT-SCIENCE & APPLICATIONS, vol. 7, 2018, doi:10.1038/s41377-018-0048-3.
APA
Liu, J., Xiong, R., Brans, T., Lippens, S., Parthoens, E., Zanacchi, F. C., … Braeckmans, K. (2018). Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy. LIGHT-SCIENCE & APPLICATIONS, 7. https://doi.org/10.1038/s41377-018-0048-3
Chicago author-date
Liu, Jing, Ranhua Xiong, Toon Brans, Saskia Lippens, Eef Parthoens, Francesca Cella Zanacchi, Raffaella Magrassi, et al. 2018. “Repeated Photoporation with Graphene Quantum Dots Enables Homogeneous Labeling of Live Cells with Extrinsic Markers for Fluorescence Microscopy.” LIGHT-SCIENCE & APPLICATIONS 7. https://doi.org/10.1038/s41377-018-0048-3.
Chicago author-date (all authors)
Liu, Jing, Ranhua Xiong, Toon Brans, Saskia Lippens, Eef Parthoens, Francesca Cella Zanacchi, Raffaella Magrassi, Santosh K Singh, Sreekumar Kurungot, Sabine Szunerits, Hannelore Bové, Marcel Ameloot, Juan Fraire, Eline Teirlinck, Sangram Keshari Samal, Riet De Rycke, Gaëlle Houthaeve, Stefaan De Smedt, Rabah Boukherroub, and Kevin Braeckmans. 2018. “Repeated Photoporation with Graphene Quantum Dots Enables Homogeneous Labeling of Live Cells with Extrinsic Markers for Fluorescence Microscopy.” LIGHT-SCIENCE & APPLICATIONS 7. doi:10.1038/s41377-018-0048-3.
Vancouver
1.
Liu J, Xiong R, Brans T, Lippens S, Parthoens E, Zanacchi FC, et al. Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy. LIGHT-SCIENCE & APPLICATIONS. 2018;7.
IEEE
[1]
J. Liu et al., “Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy,” LIGHT-SCIENCE & APPLICATIONS, vol. 7, 2018.
@article{8571697,
  abstract     = {{In the replacement of genetic probes, there is increasing interest in labeling living cells with high-quality extrinsic labels, which avoid over-expression artifacts and are available in a wide spectral range. This calls for a broadly applicable technology that can deliver such labels unambiguously to the cytosol of living cells. Here, we demonstrate that nanoparticle-sensitized photoporation can be used to this end as an emerging intracellular delivery technique. We replace the traditionally used gold nanoparticles with graphene nanoparticles as photothermal sensitizers to permeabilize the cell membrane upon laser irradiation. We demonstrate that the enhanced thermal stability of graphene quantum dots allows the formation of multiple vapor nanobubbles upon irradiation with short laser pulses, allowing the delivery of a variety of extrinsic cell labels efficiently and homogeneously into live cells. We demonstrate high-quality time-lapse imaging with confocal, total internal reflection fluorescence (TIRF), and Airyscan super-resolution microscopy. As the entire procedure is readily compatible with fluorescence (super resolution) microscopy, photoporation with graphene quantum dots has the potential to become the long-awaited generic platform for controlled intracellular delivery of fluorescent labels for live-cell imaging.}},
  articleno    = {{47}},
  author       = {{Liu, Jing and Xiong, Ranhua and Brans, Toon and Lippens, Saskia and Parthoens, Eef and Zanacchi, Francesca Cella and Magrassi, Raffaella and Singh, Santosh K and Kurungot, Sreekumar and Szunerits, Sabine and Bové, Hannelore and Ameloot, Marcel and Fraire, Juan and Teirlinck, Eline and Samal, Sangram Keshari and De Rycke, Riet and Houthaeve, Gaëlle and De Smedt, Stefaan and Boukherroub, Rabah and Braeckmans, Kevin}},
  issn         = {{2047-7538}},
  journal      = {{LIGHT-SCIENCE & APPLICATIONS}},
  keywords     = {{TAG FUSION PROTEINS,INTRACELLULAR DELIVERY,GOLD NANOPARTICLES,LIVING CELLS,MAMMALIAN-CELLS,NANOMATERIALS,TRAFFICKING,NANOBUBBLES,NANOBODIES,MOLECULES}},
  language     = {{eng}},
  pages        = {{10}},
  title        = {{Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy}},
  url          = {{http://dx.doi.org/10.1038/s41377-018-0048-3}},
  volume       = {{7}},
  year         = {{2018}},
}
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