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Influence of three-dimensional lung epithelial cells and interspecies interactions on antibiotic efficacy against Mycobacterium abscessus and Pseudomonas aeruginosa

Graciela Rodriguez-Sevilla, Charlotte Rigauts, Eva Vandeplassche, Lisa Ostyn (UGent) , Ignacio Mahillo-Fernandez, Jaime Esteban, Concepcion Perez-Jorge Peremarch, Tom Coenye (UGent) and Aurélie Crabbé (UGent)
(2018) PATHOGENS AND DISEASE. 76(4).
Author
Organization
Abstract
Mycobacterium abscessus lung infection is a major health problem for cystic fibrosis (CF) patients. Understanding the in vivo factors that influence the outcome of therapy may help addressing the poor correlation between in vitro and in vivo antibiotic efficacy. We evaluated the influence of interspecies interactions and lung epithelial cells on antibiotic efficacy. Therefore, single and dual-species biofilms of M. abscessus and a major CF pathogen (Pseudomonas aeruginosa) were cultured on a plastic surface or on in vivo-like three-dimensional (3-D) lung epithelial cells, and the activity of antibiotics (colistin, amikacin, clarithromycin, ceftazidime) in inhibiting biofilm formation was evaluated. Using the most physiologically relevant model (dual-species biofilms on 3-D cells), we observed that treatment with antibiotics during biofilm development inhibited P. aeruginosa but not M. abscessus biofilms, resulting in a competitive advantage for the latter. Clarithromycin efficacy against P. aeruginosa was inhibited by 3-D lung cells. In addition, biofilm induction of M. abscessus was observed by certain antibiotics on plastic but not on 3-D cells. Pseudomonas aeruginosa influenced the efficacy of certain antibiotics against M. abscessus, but not vice versa. In conclusion, these results suggest a role of host cells and interspecies interactions in bacterial responses to antimicrobials.
Keywords
CYSTIC-FIBROSIS PATIENTS, NONTUBERCULOUS MYCOBACTERIA, IN-VITRO, INFECTIONS, HOST, BIOFILMS, COMPLEX, MODEL, EPIDEMIOLOGY, PATHOGENESIS, biofilm, Mycobacterium abscessus, Pseudomonas aeruginosa, cells

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Citation

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MLA
Rodriguez-Sevilla, Graciela, et al. “Influence of Three-Dimensional Lung Epithelial Cells and Interspecies Interactions on Antibiotic Efficacy against Mycobacterium Abscessus and Pseudomonas Aeruginosa.” PATHOGENS AND DISEASE, vol. 76, no. 4, 2018, doi:10.1093/femspd/fty034.
APA
Rodriguez-Sevilla, G., Rigauts, C., Vandeplassche, E., Ostyn, L., Mahillo-Fernandez, I., Esteban, J., … Crabbé, A. (2018). Influence of three-dimensional lung epithelial cells and interspecies interactions on antibiotic efficacy against Mycobacterium abscessus and Pseudomonas aeruginosa. PATHOGENS AND DISEASE, 76(4). https://doi.org/10.1093/femspd/fty034
Chicago author-date
Rodriguez-Sevilla, Graciela, Charlotte Rigauts, Eva Vandeplassche, Lisa Ostyn, Ignacio Mahillo-Fernandez, Jaime Esteban, Concepcion Perez-Jorge Peremarch, Tom Coenye, and Aurélie Crabbé. 2018. “Influence of Three-Dimensional Lung Epithelial Cells and Interspecies Interactions on Antibiotic Efficacy against Mycobacterium Abscessus and Pseudomonas Aeruginosa.” PATHOGENS AND DISEASE 76 (4). https://doi.org/10.1093/femspd/fty034.
Chicago author-date (all authors)
Rodriguez-Sevilla, Graciela, Charlotte Rigauts, Eva Vandeplassche, Lisa Ostyn, Ignacio Mahillo-Fernandez, Jaime Esteban, Concepcion Perez-Jorge Peremarch, Tom Coenye, and Aurélie Crabbé. 2018. “Influence of Three-Dimensional Lung Epithelial Cells and Interspecies Interactions on Antibiotic Efficacy against Mycobacterium Abscessus and Pseudomonas Aeruginosa.” PATHOGENS AND DISEASE 76 (4). doi:10.1093/femspd/fty034.
Vancouver
1.
Rodriguez-Sevilla G, Rigauts C, Vandeplassche E, Ostyn L, Mahillo-Fernandez I, Esteban J, et al. Influence of three-dimensional lung epithelial cells and interspecies interactions on antibiotic efficacy against Mycobacterium abscessus and Pseudomonas aeruginosa. PATHOGENS AND DISEASE. 2018;76(4).
IEEE
[1]
G. Rodriguez-Sevilla et al., “Influence of three-dimensional lung epithelial cells and interspecies interactions on antibiotic efficacy against Mycobacterium abscessus and Pseudomonas aeruginosa,” PATHOGENS AND DISEASE, vol. 76, no. 4, 2018.
@article{8571441,
  abstract     = {{Mycobacterium abscessus lung infection is a major health problem for cystic fibrosis (CF) patients. Understanding the in vivo factors that influence the outcome of therapy may help addressing the poor correlation between in vitro and in vivo antibiotic efficacy. We evaluated the influence of interspecies interactions and lung epithelial cells on antibiotic efficacy. Therefore, single and dual-species biofilms of M. abscessus and a major CF pathogen (Pseudomonas aeruginosa) were cultured on a plastic surface or on in vivo-like three-dimensional (3-D) lung epithelial cells, and the activity of antibiotics (colistin, amikacin, clarithromycin, ceftazidime) in inhibiting biofilm formation was evaluated. Using the most physiologically relevant model (dual-species biofilms on 3-D cells), we observed that treatment with antibiotics during biofilm development inhibited P. aeruginosa but not M. abscessus biofilms, resulting in a competitive advantage for the latter. Clarithromycin efficacy against P. aeruginosa was inhibited by 3-D lung cells. In addition, biofilm induction of M. abscessus was observed by certain antibiotics on plastic but not on 3-D cells. Pseudomonas aeruginosa influenced the efficacy of certain antibiotics against M. abscessus, but not vice versa. In conclusion, these results suggest a role of host cells and interspecies interactions in bacterial responses to antimicrobials.}},
  articleno    = {{fty034}},
  author       = {{Rodriguez-Sevilla, Graciela and Rigauts, Charlotte and Vandeplassche, Eva and Ostyn, Lisa and Mahillo-Fernandez, Ignacio and Esteban, Jaime and Perez-Jorge Peremarch, Concepcion and Coenye, Tom and Crabbé, Aurélie}},
  issn         = {{2049-632X}},
  journal      = {{PATHOGENS AND DISEASE}},
  keywords     = {{CYSTIC-FIBROSIS PATIENTS,NONTUBERCULOUS MYCOBACTERIA,IN-VITRO,INFECTIONS,HOST,BIOFILMS,COMPLEX,MODEL,EPIDEMIOLOGY,PATHOGENESIS,biofilm,Mycobacterium abscessus,Pseudomonas aeruginosa,cells}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{8}},
  title        = {{Influence of three-dimensional lung epithelial cells and interspecies interactions on antibiotic efficacy against Mycobacterium abscessus and Pseudomonas aeruginosa}},
  url          = {{http://dx.doi.org/10.1093/femspd/fty034}},
  volume       = {{76}},
  year         = {{2018}},
}
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