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Evaluation of the Roche® Elecsys and the Diasorin® Liaison S100 kits in the management of mild head injury in the emergency room

(2018) CLINICAL BIOCHEMISTRY. 52. p.123-130
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Abstract
Introduction: The aim of this single-center prospective study is to compare two commercially available S100 beta kits (the Roche (R) Elecsys and the Diasorin (R) Liaison S100 kits) in terms of analytical and clinical performances in a population admitted in the emergency room for mild traumatic brain injury (mTBI). Material and method: 110 patients were enrolled from September 2014 to May 2015. Blood sample draws were performed within 3 h after head trauma and the study population was split into pediatric and adult sub-populations (> 18 years of age). Results: Although both kits correlated well, we observed a significant difference in terms of S100 beta levels (P value < 0.05) in both subpopulations. In the pediatric subpopulation, both kits showed elevated S100 beta levels for the only patient (3.5%) who displayed abnormal findings on a CT-scan. However, we observed a poor agreement between both kits (Cohen's kappa = 0.345, P value = 0.077). In the adult subpopulation, a total of 10 patients (12.2%) had abnormal head computed tomography scans. Using the Roche (R) (cut off = 0.1 mu g/L) and the Diasorin (R) (cut off = 0.15 mu g/L) S100 beta kits, brain injuries were detected with a sensitivity of 100% (95% CI: 65-100%) and 100% (95% CI: 63-100%) and a specificity of 15.28% (95% CI: 7.9-25.7%) and 24.64% (95% CI: 15-36.5) respectively. Finally, a moderate agreement was concluded between both kits (Cohen's kappa = 0.569, P value = 0.001). Conclusion: Although a good correlation could be found between both kits, emergency physicians should be aware of discrepancies observed between both methods, making those immunoassays not interchangeable. Furthermore, more studies are still needed to validate cut off used according to technique and to age, especially in the population below the age of 2 years.
Keywords
TRAUMATIC BRAIN-INJURY, NEURON-SPECIFIC ENOLASE, SERUM S-100B PROTEIN, COMPUTED-TOMOGRAPHY, CLINICAL UTILITY, REFERENCE RANGES, CT, ADULTS, CHILDREN, DAMAGE

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Citation

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Chicago
Delefortrie, Quentin, François Lejeune, Benjamin Kerzmann, Raphael Levy, Jean-François Adam, Thierry Sottiaux, Alexandre Grimmelprez, Patrick Vankerkhoven, and Saïd Hachimi Idrissi. 2018. “Evaluation of the Roche® Elecsys and the Diasorin® Liaison S100 Kits in the Management of Mild Head Injury in the Emergency Room.” Clinical Biochemistry 52: 123–130.
APA
Delefortrie, Quentin, Lejeune, F., Kerzmann, B., Levy, R., Adam, J.-F., Sottiaux, T., Grimmelprez, A., et al. (2018). Evaluation of the Roche® Elecsys and the Diasorin® Liaison S100 kits in the management of mild head injury in the emergency room. CLINICAL BIOCHEMISTRY, 52, 123–130.
Vancouver
1.
Delefortrie Q, Lejeune F, Kerzmann B, Levy R, Adam J-F, Sottiaux T, et al. Evaluation of the Roche® Elecsys and the Diasorin® Liaison S100 kits in the management of mild head injury in the emergency room. CLINICAL BIOCHEMISTRY. 2018;52:123–30.
MLA
Delefortrie, Quentin, François Lejeune, Benjamin Kerzmann, et al. “Evaluation of the Roche® Elecsys and the Diasorin® Liaison S100 Kits in the Management of Mild Head Injury in the Emergency Room.” CLINICAL BIOCHEMISTRY 52 (2018): 123–130. Print.
@article{8571361,
  abstract     = {Introduction: The aim of this single-center prospective study is to compare two commercially available S100 beta kits (the Roche (R) Elecsys and the Diasorin (R) Liaison S100 kits) in terms of analytical and clinical performances in a population admitted in the emergency room for mild traumatic brain injury (mTBI). 
Material and method: 110 patients were enrolled from September 2014 to May 2015. Blood sample draws were performed within 3 h after head trauma and the study population was split into pediatric and adult sub-populations (> 18 years of age). 
Results: Although both kits correlated well, we observed a significant difference in terms of S100 beta levels (P value < 0.05) in both subpopulations. 
In the pediatric subpopulation, both kits showed elevated S100 beta levels for the only patient (3.5%) who displayed abnormal findings on a CT-scan. However, we observed a poor agreement between both kits (Cohen's kappa = 0.345, P value = 0.077). 
In the adult subpopulation, a total of 10 patients (12.2%) had abnormal head computed tomography scans. Using the Roche (R) (cut off = 0.1 mu g/L) and the Diasorin (R) (cut off = 0.15 mu g/L) S100 beta kits, brain injuries were detected with a sensitivity of 100% (95% CI: 65-100%) and 100% (95% CI: 63-100%) and a specificity of 15.28% (95% CI: 7.9-25.7%) and 24.64% (95% CI: 15-36.5) respectively. Finally, a moderate agreement was concluded between both kits (Cohen's kappa = 0.569, P value = 0.001). 
Conclusion: Although a good correlation could be found between both kits, emergency physicians should be aware of discrepancies observed between both methods, making those immunoassays not interchangeable. Furthermore, more studies are still needed to validate cut off used according to technique and to age, especially in the population below the age of 2 years.},
  author       = {Delefortrie, Quentin and Lejeune, François and Kerzmann, Benjamin and Levy, Raphael and Adam, Jean-François and Sottiaux, Thierry and Grimmelprez, Alexandre and Vankerkhoven, Patrick and Hachimi Idrissi, Saïd},
  issn         = {0009-9120},
  journal      = {CLINICAL BIOCHEMISTRY},
  keywords     = {TRAUMATIC BRAIN-INJURY,NEURON-SPECIFIC ENOLASE,SERUM S-100B PROTEIN,COMPUTED-TOMOGRAPHY,CLINICAL UTILITY,REFERENCE RANGES,CT,ADULTS,CHILDREN,DAMAGE},
  language     = {eng},
  pages        = {123--130},
  title        = {Evaluation of the Roche® Elecsys and the Diasorin® Liaison S100 kits in the management of mild head injury in the emergency room},
  url          = {http://dx.doi.org/10.1016/j.clinbiochem.2017.11.004},
  volume       = {52},
  year         = {2018},
}

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