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Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15S1-CD163 and PK15S10-CD163 cells

Jiexiong Xie (UGent) , Isaura Christiaens (UGent) , Bo Yang (UGent) , Ivan Trus (UGent) , Bert Devriendt (UGent) , Tingting Cui (UGent) , Ruifang Wei (UGent) and Hans Nauwynck (UGent)
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Abstract
Cellular entry mediators define whether the cell is permissive to PRRSV infection. Porcine sialoadhesin (pSn, Siglec-1) and CD163 are main entry mediators facilitating infection of porcine macrophages by PRRSV. Recently, Siglec-10 was demonstrated to be an alternative receptor for PRRSV. To examine if virulence and pathogenicity of PRRSV strains could be correlated with the use of different Siglecs, a PK15 cell line recombinantly expressing Siglec-1 and CD163 (PK15(S1-CD163)) and a PK15 cell line recombinantly expressing Siglec-10 and CD163 (PK15(S10-CD163)) were used to compare the virus replication of 7 genotype 1 subtype 1 strains (G1s1), 2 genotype 1 subtype 3 (G1s3) strains and 5 genotype 2 (G2) strains. Some strains (08VA (G1s1), 13V117 (G1s1), 17V035 (G1s1), VR2332 (G2)) were poor virus producers (<10(4) TCID50/mL), while other strains (07V063 (G1s1), 13V091 (G1s1), Su1-Bel (G1s3), MN-184 (G2), Korea17 (G2) and SDSU-73 (G2)) easily grew up to >= 10(6) TCID50/mL. PK15(S10-CD163) cells exhibited a higher efficiency in virus production per infected cell than the PK15(S1-CD163) cells. The G1s1 strains LV and 07V063 infected more cells in the PK15(S1-CD163), whereas the 94V360 and 08VA strains preferred PK15(S10-CD163). The highly virulent G1s3 strains Lena and Su1-Bel showed a strong preference for PK15(S1-CD163). The G2 strains MN-184, SDSU-73, Korea17 had a much higher infection rate in PK15(S10-CD163), while the reference strain VR2332 and the NADC30 strain had a slight preference for -PK15(S1-CD163). Differences in receptor use may influence the outcome of a PRRSV infection in pigs and explain in part the virulence/pathogenicity of PRRSV strains.
Keywords
RESPIRATORY SYNDROME VIRUS, PORCINE ALVEOLAR MACROPHAGES, DENDRITIC CELLS, IDENTIFICATION, REPLICATION, CD163, SIALOADHESIN, PIGS, SUSCEPTIBILITY, ANTIBODIES

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Chicago
Xie, Jiexiong, Isaura Christiaens, Bo Yang, Ivan Trus, Bert Devriendt, Tingting Cui, Ruifang Wei, and Hans Nauwynck. 2018. “Preferential Use of Siglec-1 or Siglec-10 by Type 1 and Type 2 PRRSV Strains to Infect PK15S1-CD163 and PK15S10-CD163 Cells.” Veterinary Research 49.
APA
Xie, Jiexiong, Christiaens, I., Yang, B., Trus, I., Devriendt, B., Cui, T., Wei, R., et al. (2018). Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15S1-CD163 and PK15S10-CD163 cells. VETERINARY RESEARCH, 49.
Vancouver
1.
Xie J, Christiaens I, Yang B, Trus I, Devriendt B, Cui T, et al. Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15S1-CD163 and PK15S10-CD163 cells. VETERINARY RESEARCH. 2018;49.
MLA
Xie, Jiexiong, Isaura Christiaens, Bo Yang, et al. “Preferential Use of Siglec-1 or Siglec-10 by Type 1 and Type 2 PRRSV Strains to Infect PK15S1-CD163 and PK15S10-CD163 Cells.” VETERINARY RESEARCH 49 (2018): n. pag. Print.
@article{8571271,
  abstract     = {Cellular entry mediators define whether the cell is permissive to PRRSV infection. Porcine sialoadhesin (pSn, Siglec-1) and CD163 are main entry mediators facilitating infection of porcine macrophages by PRRSV. Recently, Siglec-10 was demonstrated to be an alternative receptor for PRRSV. To examine if virulence and pathogenicity of PRRSV strains could be correlated with the use of different Siglecs, a PK15 cell line recombinantly expressing Siglec-1 and CD163 (PK15(S1-CD163)) and a PK15 cell line recombinantly expressing Siglec-10 and CD163 (PK15(S10-CD163)) were used to compare the virus replication of 7 genotype 1 subtype 1 strains (G1s1), 2 genotype 1 subtype 3 (G1s3) strains and 5 genotype 2 (G2) strains. Some strains (08VA (G1s1), 13V117 (G1s1), 17V035 (G1s1), VR2332 (G2)) were poor virus producers ({\textlangle}10(4) TCID50/mL), while other strains (07V063 (G1s1), 13V091 (G1s1), Su1-Bel (G1s3), MN-184 (G2), Korea17 (G2) and SDSU-73 (G2)) easily grew up to {\textrangle}= 10(6) TCID50/mL. PK15(S10-CD163) cells exhibited a higher efficiency in virus production per infected cell than the PK15(S1-CD163) cells. The G1s1 strains LV and 07V063 infected more cells in the PK15(S1-CD163), whereas the 94V360 and 08VA strains preferred PK15(S10-CD163). The highly virulent G1s3 strains Lena and Su1-Bel showed a strong preference for PK15(S1-CD163). The G2 strains MN-184, SDSU-73, Korea17 had a much higher infection rate in PK15(S10-CD163), while the reference strain VR2332 and the NADC30 strain had a slight preference for -PK15(S1-CD163). Differences in receptor use may influence the outcome of a PRRSV infection in pigs and explain in part the virulence/pathogenicity of PRRSV strains.},
  articleno    = {67},
  author       = {Xie, Jiexiong and Christiaens, Isaura and Yang, Bo and Trus, Ivan and Devriendt, Bert and Cui, Tingting and Wei, Ruifang and Nauwynck, Hans},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  language     = {eng},
  pages        = {13},
  title        = {Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15S1-CD163 and PK15S10-CD163 cells},
  url          = {http://dx.doi.org/10.1186/s13567-018-0569-z},
  volume       = {49},
  year         = {2018},
}

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