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The proton sponge hypothesis : fable or fact?

Lotte Vermeulen (UGent) , Stefaan De Smedt (UGent) , Katrien Remaut (UGent) and Kevin Braeckmans (UGent)
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Organization
Project
Center for nano- and biophotonics (NB-Photonics)
Abstract
In non-viral gene therapy, cationic polymers and lipids are frequently used to encapsulate macromolecular therapeutics into nanoparticles. During their journey to deliver the cargo to the intended intracellular target, many biological barriers need to be overcome. One of the major bottlenecks for efficient transfection is the endosomal barrier since nanoparticles often remain entrapped inside endosomes and are trafficked towards the lysosomes where the cargo is degraded. For cationic polymers, the proton sponge hypothesis was introduced in the late '90s as a way to explain their endosomal escape properties. However, to date, no consensus has been reached in the scientific community about the validity of this hypothesis due to many contradictory reports. Here we review the sometimes conflicting reports that have been published on the proton sponge hypothesis. We also discuss membrane destabilization and polymer swelling as additional factors that might influence endosomal escape of polyplexes. Based on the key publications on this subject, we aim to launch a consensus on the role of the proton sponge hypothesis in endosomal escape.
Keywords
Nanomedicine, Non-viral gene therapy, Cationic polymers, Endosomal escape, Proton sponge hypothesis, EFFICIENT GENE-TRANSFER, ENDOSOMAL ESCAPE, INTRACELLULAR DELIVERY, CHLORIDE ACCUMULATION, DNA POLYPLEXES, POLYETHYLENIMINE, CELLS, COMPLEXES, TRAFFICKING, POLYMERS

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Citation

Please use this url to cite or link to this publication:

MLA
Vermeulen, Lotte et al. “The Proton Sponge Hypothesis : Fable or Fact?” EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS 129 (2018): 184–190. Print.
APA
Vermeulen, Lotte, De Smedt, S., Remaut, K., & Braeckmans, K. (2018). The proton sponge hypothesis : fable or fact? EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 129, 184–190.
Chicago author-date
Vermeulen, Lotte, Stefaan De Smedt, Katrien Remaut, and Kevin Braeckmans. 2018. “The Proton Sponge Hypothesis : Fable or Fact?” European Journal of Pharmaceutics and Biopharmaceutics 129: 184–190.
Chicago author-date (all authors)
Vermeulen, Lotte, Stefaan De Smedt, Katrien Remaut, and Kevin Braeckmans. 2018. “The Proton Sponge Hypothesis : Fable or Fact?” European Journal of Pharmaceutics and Biopharmaceutics 129: 184–190.
Vancouver
1.
Vermeulen L, De Smedt S, Remaut K, Braeckmans K. The proton sponge hypothesis : fable or fact? EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS. 2018;129:184–90.
IEEE
[1]
L. Vermeulen, S. De Smedt, K. Remaut, and K. Braeckmans, “The proton sponge hypothesis : fable or fact?,” EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, vol. 129, pp. 184–190, 2018.
@article{8570820,
  abstract     = {In non-viral gene therapy, cationic polymers and lipids are frequently used to encapsulate macromolecular therapeutics into nanoparticles. During their journey to deliver the cargo to the intended intracellular target, many biological barriers need to be overcome. One of the major bottlenecks for efficient transfection is the endosomal barrier since nanoparticles often remain entrapped inside endosomes and are trafficked towards the lysosomes where the cargo is degraded. For cationic polymers, the proton sponge hypothesis was introduced in the late '90s as a way to explain their endosomal escape properties. However, to date, no consensus has been reached in the scientific community about the validity of this hypothesis due to many contradictory reports. Here we review the sometimes conflicting reports that have been published on the proton sponge hypothesis. We also discuss membrane destabilization and polymer swelling as additional factors that might influence endosomal escape of polyplexes. Based on the key publications on this subject, we aim to launch a consensus on the role of the proton sponge hypothesis in endosomal escape.},
  author       = {Vermeulen, Lotte and De Smedt, Stefaan and Remaut, Katrien and Braeckmans, Kevin},
  issn         = {0939-6411},
  journal      = {EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS},
  keywords     = {Nanomedicine,Non-viral gene therapy,Cationic polymers,Endosomal escape,Proton sponge hypothesis,EFFICIENT GENE-TRANSFER,ENDOSOMAL ESCAPE,INTRACELLULAR DELIVERY,CHLORIDE ACCUMULATION,DNA POLYPLEXES,POLYETHYLENIMINE,CELLS,COMPLEXES,TRAFFICKING,POLYMERS},
  language     = {eng},
  pages        = {184--190},
  title        = {The proton sponge hypothesis : fable or fact?},
  url          = {http://dx.doi.org/10.1016/j.ejpb.2018.05.034},
  volume       = {129},
  year         = {2018},
}

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