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Inulin chain length modification using a transgenic approach opening new perspectives for chicory

(2018) 3 BIOTECH. 8(8).
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Abstract
Chicory capable of synthesizing long-chain inulin is of great interest. During the growing season, the sucrose-sucrose 1-fructosyltransferase (1-SST) activity is vital for production of long-chain inulin in chicory. With the purpose to increase inulin chain length, we employed Agrobacterium-mediated transformation method. Transgenic chicory plants (Cichorium intybus L. var. sativum) cv. 'Melci' has been developed to overexpress sucrose-sucrose 1-fructosyltransferase (1-SST) under the control of the CaMV 35S promoter. The integration of the T-DNA into the plant genome was confirmed by PCR on genomic DNA using gene-specific primers. Quantification of the 1-SST transcript expression level revealed that transgenic plants showed higher 1-SST expression than those in non-transgenic plants. Further analyses proved that the fructan content of the roots significantly increased in the transgenic plants. These results revealed that overexpression of the 1-SST, the key gene in inulin biosynthesis in chicory, might serve as a novel approach to develop plants with the long-chain inulin content.
Keywords
Inulin, Chicory, Overexpress, Transgene: CICHORIUM-INTYBUS L, FRUCTAN ACCUMULATION, FUNCTIONAL-ANALYSIS, MASS FINGERPRINT, HARVEST DATE, IN-VITRO, PLANTS, ROOTS, CLONING, PURIFICATION

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Citation

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Chicago
Maroufi, Asad, Mansour Karimi, Khosro Mehdikhanlou, and Marc De Loose. 2018. “Inulin Chain Length Modification Using a Transgenic Approach Opening New Perspectives for Chicory.” 3 BIOTECH 8 (8).
APA
Maroufi, Asad, Karimi, M., Mehdikhanlou, K., & De Loose, M. (2018). Inulin chain length modification using a transgenic approach opening new perspectives for chicory. 3 BIOTECH, 8(8).
Vancouver
1.
Maroufi A, Karimi M, Mehdikhanlou K, De Loose M. Inulin chain length modification using a transgenic approach opening new perspectives for chicory. 3 BIOTECH. 2018;8(8).
MLA
Maroufi, Asad, Mansour Karimi, Khosro Mehdikhanlou, et al. “Inulin Chain Length Modification Using a Transgenic Approach Opening New Perspectives for Chicory.” 3 BIOTECH 8.8 (2018): n. pag. Print.
@article{8570745,
  abstract     = {Chicory capable of synthesizing long-chain inulin is of great interest. During the growing season, the sucrose-sucrose 1-fructosyltransferase (1-SST) activity is vital for production of long-chain inulin in chicory. With the purpose to increase inulin chain length, we employed Agrobacterium-mediated transformation method. Transgenic chicory plants (Cichorium intybus L. var. sativum) cv. 'Melci' has been developed to overexpress sucrose-sucrose 1-fructosyltransferase (1-SST) under the control of the CaMV 35S promoter. The integration of the T-DNA into the plant genome was confirmed by PCR on genomic DNA using gene-specific primers. Quantification of the 1-SST transcript expression level revealed that transgenic plants showed higher 1-SST expression than those in non-transgenic plants. Further analyses proved that the fructan content of the roots significantly increased in the transgenic plants. These results revealed that overexpression of the 1-SST, the key gene in inulin biosynthesis in chicory, might serve as a novel approach to develop plants with the long-chain inulin content.},
  articleno    = {349},
  author       = {Maroufi, Asad and Karimi, Mansour and Mehdikhanlou, Khosro and De Loose, Marc},
  issn         = {2190-572X},
  journal      = {3 BIOTECH},
  language     = {eng},
  number       = {8},
  pages        = {8},
  title        = {Inulin chain length modification using a transgenic approach opening new perspectives for chicory},
  url          = {http://dx.doi.org/10.1007/s13205-018-1377-x},
  volume       = {8},
  year         = {2018},
}

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