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Self-protection of cytosolic malate dehydrogenase against oxidative stress in Arabidopsis

(2018) JOURNAL OF EXPERIMENTAL BOTANY. 69(14). p.3491-3505
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Abstract
Plant malate dehydrogenase (MDH) isoforms are found in different cell compartments and function in key metabolic pathways. It is well known that the chloroplastic NADP-dependent MDH activities are strictly redox regulated and controlled by light. However, redox dependence of other NAD-dependent MDH isoforms have been less studied. Here, we show by in vitro biochemical characterization that the major cytosolic MDH isoform (cytMDH1) is sensitive to H2O2 through sulfur oxidation of cysteines and methionines. CytMDH1 oxidation affects the kinetics, secondary structure, and thermodynamic stability of cytMDH1. Moreover, MS analyses and comparison of crystal structures between the reduced and H2O2-treated cytMDH1 further show that thioredoxin-reversible homodimerization of cytMDH1 through Cys330 disulfide formation protects the protein from overoxidation. Consistently, we found that cytosolic thioredoxins interact specifically with cytMDH in a yeast two-hybrid system. Importantly, we also show that cytosolic and chloroplastic, but not mitochondrial NAD-MDH activities are sensitive to H2O2 stress in Arabidopsis. NAD-MDH activities decreased both in a catalase2 mutant and in an NADP-thioredoxin reductase mutant, emphasizing the importance of the thioredoxin-reducing system to protect MDH from oxidation in vivo. We propose that the redox switch of the MDH activity contributes to adapt the cell metabolism to environmental constraints.
Keywords
CytMDH1, dimerization, H2O2-triggered oxdation, overoxidation, sulfenic acid, thioredoxin, STRUCTURAL BASIS, GENE-EXPRESSION, CELL-DEATH, THIOREDOXIN, THALIANA, PROTEINS, PLANTS, IDENTIFICATION, REFINEMENT, REDUCTION

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Citation

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Chicago
Huang, Jingjing, Adnan Khan Niazi, David Young, Leonardo Astolfi Rosado, Didier Vertommen, Nandita Bodra, Mohamed Ragab Abdelgawwad, et al. 2018. “Self-protection of Cytosolic Malate Dehydrogenase Against Oxidative Stress in Arabidopsis.” Journal of Experimental Botany 69 (14): 3491–3505.
APA
Huang, Jingjing, Niazi, A. K., Young, D., Rosado, L. A., Vertommen, D., Bodra, N., Abdelgawwad, M. R., et al. (2018). Self-protection of cytosolic malate dehydrogenase against oxidative stress in Arabidopsis. JOURNAL OF EXPERIMENTAL BOTANY, 69(14), 3491–3505.
Vancouver
1.
Huang J, Niazi AK, Young D, Rosado LA, Vertommen D, Bodra N, et al. Self-protection of cytosolic malate dehydrogenase against oxidative stress in Arabidopsis. JOURNAL OF EXPERIMENTAL BOTANY. 2018;69(14):3491–505.
MLA
Huang, Jingjing, Adnan Khan Niazi, David Young, et al. “Self-protection of Cytosolic Malate Dehydrogenase Against Oxidative Stress in Arabidopsis.” JOURNAL OF EXPERIMENTAL BOTANY 69.14 (2018): 3491–3505. Print.
@article{8570736,
  abstract     = {Plant malate dehydrogenase (MDH) isoforms are found in different cell compartments and function in key metabolic pathways. It is well known that the chloroplastic NADP-dependent MDH activities are strictly redox regulated and controlled by light. However, redox dependence of other NAD-dependent MDH isoforms have been less studied. Here, we show by in vitro biochemical characterization that the major cytosolic MDH isoform (cytMDH1) is sensitive to H2O2 through sulfur oxidation of cysteines and methionines. CytMDH1 oxidation affects the kinetics, secondary structure, and thermodynamic stability of cytMDH1. Moreover, MS analyses and comparison of crystal structures between the reduced and H2O2-treated cytMDH1 further show that thioredoxin-reversible homodimerization of cytMDH1 through Cys330 disulfide formation protects the protein from overoxidation. Consistently, we found that cytosolic thioredoxins interact specifically with cytMDH in a yeast two-hybrid system. Importantly, we also show that cytosolic and chloroplastic, but not mitochondrial NAD-MDH activities are sensitive to H2O2 stress in Arabidopsis. NAD-MDH activities decreased both in a catalase2 mutant and in an NADP-thioredoxin reductase mutant, emphasizing the importance of the thioredoxin-reducing system to protect MDH from oxidation in vivo. We propose that the redox switch of the MDH activity contributes to adapt the cell metabolism to environmental constraints.},
  author       = {Huang, Jingjing and Niazi, Adnan Khan and Young, David and Rosado, Leonardo Astolfi and Vertommen, Didier and Bodra, Nandita and Abdelgawwad, Mohamed Ragab and Vignols, Florence and Wei, Bo and Wahni, Khadija and Bashandy, Talaat and Bariat, Laetitia and Van Breusegem, Frank and Messens, Joris and Reichheld, Jean-Philippe},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keyword      = {CytMDH1,dimerization,H2O2-triggered oxdation,overoxidation,sulfenic acid,thioredoxin,STRUCTURAL BASIS,GENE-EXPRESSION,CELL-DEATH,THIOREDOXIN,THALIANA,PROTEINS,PLANTS,IDENTIFICATION,REFINEMENT,REDUCTION},
  language     = {eng},
  number       = {14},
  pages        = {3491--3505},
  title        = {Self-protection of cytosolic malate dehydrogenase against oxidative stress in Arabidopsis},
  url          = {http://dx.doi.org/10.1093/jxb/erx396},
  volume       = {69},
  year         = {2018},
}

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