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Discovering cellular protein-protein interactions : technological strategies and opportunities

Kevin Titeca (UGent) , Irma Lemmens (UGent) , Jan Tavernier (UGent) and Sven Eyckerman (UGent)
(2019) MASS SPECTROMETRY REVIEWS. 38(1). p.79-111
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Abstract
The analysis of protein interaction networks is one of the key challenges in the study of biology. It connects genotypes to phenotypes, and disruption often leads to diseases. Hence, many technologies have been developed to study protein-protein interactions (PPIs) in a cellular context. The expansion of the PPI technology toolbox however complicates the selection of optimal approaches for diverse biological questions. This review gives an overview of the binary and co-complex technologies, with the former evaluating the interaction of two co-expressed genetically tagged proteins, and the latter only needing the expression of a single tagged protein or no tagged proteins at all. Mass spectrometry is crucial for some binary and all co-complex technologies. After the detailed description of the different technologies, the review compares their unique specifications, advantages, disadvantages, and applicability, while highlighting opportunities for further advancements.
Keywords
binary interactomics, co-complex interactomics, interactomics technologies, protein-protein interactions, TANDEM AFFINITY PURIFICATION, RESONANCE ENERGY-TRANSFER, CHEMICAL CROSS-LINKING, LUCIFERASE COMPLEMENTATION ASSAY, QUANTITATIVE PROTEOMIC ANALYSIS, ENZYME-MEDIATED ACTIVATION, MEMBRANE 2-HYBRID ASSAY, MASS-SPECTROMETRY DATA, LIVING CELLS, LABEL-FREE

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Chicago
Titeca, Kevin, Irma Lemmens, Jan Tavernier, and Sven Eyckerman. 2019. “Discovering Cellular Protein-protein Interactions : Technological Strategies and Opportunities.” Mass Spectrometry Reviews 38 (1): 79–111.
APA
Titeca, Kevin, Lemmens, I., Tavernier, J., & Eyckerman, S. (2019). Discovering cellular protein-protein interactions : technological strategies and opportunities. MASS SPECTROMETRY REVIEWS, 38(1), 79–111.
Vancouver
1.
Titeca K, Lemmens I, Tavernier J, Eyckerman S. Discovering cellular protein-protein interactions : technological strategies and opportunities. MASS SPECTROMETRY REVIEWS. 2019;38(1):79–111.
MLA
Titeca, Kevin, Irma Lemmens, Jan Tavernier, et al. “Discovering Cellular Protein-protein Interactions : Technological Strategies and Opportunities.” MASS SPECTROMETRY REVIEWS 38.1 (2019): 79–111. Print.
@article{8570106,
  abstract     = {The analysis of protein interaction networks is one of the key challenges in the study of biology. It connects genotypes to phenotypes, and disruption often leads to diseases. Hence, many technologies have been developed to study protein-protein interactions (PPIs) in a cellular context. The expansion of the PPI technology toolbox however complicates the selection of optimal approaches for diverse biological questions. This review gives an overview of the binary and co-complex technologies, with the former evaluating the interaction of two co-expressed genetically tagged proteins, and the latter only needing the expression of a single tagged protein or no tagged proteins at all. Mass spectrometry is crucial for some binary and all co-complex technologies. After the detailed description of the different technologies, the review compares their unique specifications, advantages, disadvantages, and applicability, while highlighting opportunities for further advancements.},
  author       = {Titeca, Kevin and Lemmens, Irma and Tavernier, Jan and Eyckerman, Sven},
  issn         = {0277-7037},
  journal      = {MASS SPECTROMETRY REVIEWS},
  language     = {eng},
  number       = {1},
  pages        = {79--111},
  title        = {Discovering cellular protein-protein interactions : technological strategies and opportunities},
  url          = {http://dx.doi.org/10.1002/mas.21574},
  volume       = {38},
  year         = {2019},
}

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