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A novel high-throughput method for kinetic characterisation of anaerobic bioproduction strains, applied to Clostridium kluyveri

Pieter Candry (UGent) , Timothy Van Daele (UGent) , Kyrina Denis, Youri Amerlinck (UGent) , Stephen Andersen (UGent) , Ramon Ganigué (UGent) , Jan Arends (UGent) , Ingmar Nopens (UGent) and Korneel Rabaey (UGent)
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Abstract
Hexanoic acid (HA), also called caproic acid, can be used as an antimicrobial agent and as a precursor to various chemicals, such as fuels, solvents and fragrances. HA can be produced from ethanol and acetate by the mesophilic anaerobic bacterium Clostridium kluyveri, via two successive elongation steps over butyrate. A high-throughput anaerobic growth curve technique was coupled to a data analysis framework to assess growth kinetics for a range of substrate and product concentrations. Using this method, growth rates and several kinetic parameters were determined for C. kluyveri. A maximum growth rate (mu(max)) of 0.24 +/- 0.01 h(-1) was found, with a half-saturation index for acetic acid (K-S,K-AA) of 3.8 +/- 0.9 mM. Inhibition by butyric acid occurred at of 124.7 +/- 5.7 mM (K-I,K-BA), while the final product, HA, linearly inhibited growth with complete inhibition above 91.3 +/- 10.8 mM (K-HA of 10.9*10(-3) +/- 1.3*10(-3) mM(-1)) at pH = 7, indicating that the hexanoate anion also exerts toxicity. These parameters were used to create a dynamic mass-balance model for bioproduction of HA. By coupling data collection and analysis to this modelling framework, we have produced a powerful tool to assess the kinetics of anaerobic micro-organisms, demonstrated here with C. kluyveri, in order further explore the potential of micro-organisms for chemicals production.
Keywords
CAPROIC ACID PRODUCTION, CHAIN ELONGATION, REACTOR MICROBIOMES, ENERGY-METABOLISM, FATTY-ACIDS, FERMENTATION, GROWTH, MECHANISMS

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Chicago
Candry, Pieter, Timothy Van Daele, Kyrina Denis, Youri Amerlinck, Stephen Andersen, Ramon Ganigué, Jan Arends, Ingmar Nopens, and Korneel Rabaey. 2018. “A Novel High-throughput Method for Kinetic Characterisation of Anaerobic Bioproduction Strains, Applied to Clostridium Kluyveri.” Scientific Reports 8.
APA
Candry, P., Van Daele, T., Denis, K., Amerlinck, Y., Andersen, S., Ganigué, R., Arends, J., et al. (2018). A novel high-throughput method for kinetic characterisation of anaerobic bioproduction strains, applied to Clostridium kluyveri. SCIENTIFIC REPORTS, 8.
Vancouver
1.
Candry P, Van Daele T, Denis K, Amerlinck Y, Andersen S, Ganigué R, et al. A novel high-throughput method for kinetic characterisation of anaerobic bioproduction strains, applied to Clostridium kluyveri. SCIENTIFIC REPORTS. 2018;8.
MLA
Candry, Pieter, Timothy Van Daele, Kyrina Denis, et al. “A Novel High-throughput Method for Kinetic Characterisation of Anaerobic Bioproduction Strains, Applied to Clostridium Kluyveri.” SCIENTIFIC REPORTS 8 (2018): n. pag. Print.
@article{8569673,
  abstract     = {Hexanoic acid (HA), also called caproic acid, can be used as an antimicrobial agent and as a precursor to various chemicals, such as fuels, solvents and fragrances. HA can be produced from ethanol and acetate by the mesophilic anaerobic bacterium Clostridium kluyveri, via two successive elongation steps over butyrate. A high-throughput anaerobic growth curve technique was coupled to a data analysis framework to assess growth kinetics for a range of substrate and product concentrations. Using this method, growth rates and several kinetic parameters were determined for C. kluyveri. A maximum growth rate (mu(max)) of 0.24 +/- 0.01 h(-1) was found, with a half-saturation index for acetic acid (K-S,K-AA) of 3.8 +/- 0.9 mM. Inhibition by butyric acid occurred at of 124.7 +/- 5.7 mM (K-I,K-BA), while the final product, HA, linearly inhibited growth with complete inhibition above 91.3 +/- 10.8 mM (K-HA of 10.9*10(-3) +/- 1.3*10(-3) mM(-1)) at pH = 7, indicating that the hexanoate anion also exerts toxicity. These parameters were used to create a dynamic mass-balance model for bioproduction of HA. By coupling data collection and analysis to this modelling framework, we have produced a powerful tool to assess the kinetics of anaerobic micro-organisms, demonstrated here with C. kluyveri, in order further explore the potential of micro-organisms for chemicals production.},
  articleno    = {9724},
  author       = {Candry, Pieter and Van Daele, Timothy and Denis, Kyrina and Amerlinck, Youri and Andersen, Stephen and Ganigu{\'e}, Ramon and Arends, Jan and Nopens, Ingmar and Rabaey, Korneel},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  language     = {eng},
  pages        = {13},
  title        = {A novel high-throughput method for kinetic characterisation of anaerobic bioproduction strains, applied to Clostridium kluyveri},
  url          = {http://dx.doi.org/10.1038/s41598-018-27594-9},
  volume       = {8},
  year         = {2018},
}

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