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Quality control in mass spectrometry-based proteomics

(2017) MASS SPECTROMETRY REVIEWS. 37(5). p.697-711
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Abstract
Mass spectrometry is a highly complex analytical technique and mass spectrometry-based proteomics experiments can be subject to a large variability, which forms an obstacle to obtaining accurate and reproducible results. Therefore, a comprehensive and systematic approach to quality control is an essential requirement to inspire confidence in the generated results. A typical mass spectrometry experiment consists of multiple different phases including the sample preparation, liquid chromatography, mass spectrometry, and bioinformatics stages. We review potential sources of variability that can impact the results of a mass spectrometry experiment occurring in all of these steps, and we discuss how to monitor and remedy the negative influences on the experimental results. Furthermore, we describe how specialized quality control samples of varying sample complexity can be incorporated into the experimental workflow and how they can be used to rigorously assess detailed aspects of the instrument performance.
Keywords
mass spectrometry, proteomics, quality control, BOTTOM-UP PROTEOMICS, LIQUID-CHROMATOGRAPHY, LC-MS/MS, PROTEIN IDENTIFICATION, SHOTGUN PROTEOMICS, PERFORMANCE METRICS, SYSTEM SUITABILITY, QUANTITATIVE ASSESSMENT, CLINICAL PROTEOMICS, SAMPLE PREPARATION

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Chicago
Bittremieux, Wout, David L Tabb, Francis Impens, An Staes, Evy Timmerman, Lennart Martens, and Kris Laukens. 2017. “Quality Control in Mass Spectrometry-based Proteomics.” Mass Spectrometry Reviews 37 (5): 697–711.
APA
Bittremieux, W., Tabb, D. L., Impens, F., Staes, A., Timmerman, E., Martens, L., & Laukens, K. (2017). Quality control in mass spectrometry-based proteomics. MASS SPECTROMETRY REVIEWS, 37(5), 697–711.
Vancouver
1.
Bittremieux W, Tabb DL, Impens F, Staes A, Timmerman E, Martens L, et al. Quality control in mass spectrometry-based proteomics. MASS SPECTROMETRY REVIEWS. 2017;37(5):697–711.
MLA
Bittremieux, Wout, David L Tabb, Francis Impens, et al. “Quality Control in Mass Spectrometry-based Proteomics.” MASS SPECTROMETRY REVIEWS 37.5 (2017): 697–711. Print.
@article{8569647,
  abstract     = {Mass spectrometry is a highly complex analytical technique and mass spectrometry-based proteomics experiments can be subject to a large variability, which forms an obstacle to obtaining accurate and reproducible results. Therefore, a comprehensive and systematic approach to quality control is an essential requirement to inspire confidence in the generated results. A typical mass spectrometry experiment consists of multiple different phases including the sample preparation, liquid chromatography, mass spectrometry, and bioinformatics stages. We review potential sources of variability that can impact the results of a mass spectrometry experiment occurring in all of these steps, and we discuss how to monitor and remedy the negative influences on the experimental results. Furthermore, we describe how specialized quality control samples of varying sample complexity can be incorporated into the experimental workflow and how they can be used to rigorously assess detailed aspects of the instrument performance.},
  author       = {Bittremieux, Wout and Tabb, David L and Impens, Francis and Staes, An and Timmerman, Evy and Martens, Lennart and Laukens, Kris},
  issn         = {0277-7037},
  journal      = {MASS SPECTROMETRY REVIEWS},
  keyword      = {mass spectrometry,proteomics,quality control,BOTTOM-UP PROTEOMICS,LIQUID-CHROMATOGRAPHY,LC-MS/MS,PROTEIN IDENTIFICATION,SHOTGUN PROTEOMICS,PERFORMANCE METRICS,SYSTEM SUITABILITY,QUANTITATIVE ASSESSMENT,CLINICAL PROTEOMICS,SAMPLE PREPARATION},
  language     = {eng},
  number       = {5},
  pages        = {697--711},
  title        = {Quality control in mass spectrometry-based proteomics},
  url          = {http://dx.doi.org/10.1002/mas.21544},
  volume       = {37},
  year         = {2017},
}

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