Advanced search
1 file | 8.35 MB

Targeted perturbation of nuclear envelope integrity with vapor nanobubble-mediated photoporation

(2018) ACS NANO. 12(8). p.7791-7802
Author
Organization
Abstract
The nuclear envelope (NE) has long been considered to dismantle only during mitosis. However, recent observations in cancer cells and laminopathy patient cells have revealed that the NE can also transiently rupture during interphase, thereby perturbing cellular homeostasis. Although NE ruptures are promoted by mechanical force and the loss of lamins, their stochastic nature and variable frequency preclude the study of their direct downstream consequences. We have developed a method based on vapor nanobubble-mediated photoporation that allows for deliberately inducing NE ruptures in a spatiotemporally controlled manner. Our method relies on wide-field laser illumination of perinuclear gold nanoparticles, resulting in the formation of short-lived vapor nanobubbles that inflict minute mechanical damage to the NE, thus creating small pores. We demonstrate that perinuclear localization of gold nanoparticles can be achieved after endocytic uptake or electroporation-facilitated delivery and that both strategies result in NE rupture upon laser irradiation. Furthermore, we prove that photoporation-induced nuclear ruptures are transient and recapitulate hallmarks of spontaneous NE ruptures that occur in A-type lamin-depleted cells. Finally, we show that the same approach can be used to promote influx of macromolecules that are too large to passively migrate through the NE. Thus, by providing unprecedented control over nuclear compartmentalization, nuclear photoporation offers a powerful tool for both fundamental cell biology research and drug delivery applications.
Keywords
nuclear envelope, nuclear envelope rupture, nucleus, lamins, vapor nanobubbles, photoporation, gold nanoparticles, CELL-MIGRATION, LIVE CELLS, DELIVERY, LAMINOPATHIES, PROTEINS, RUPTURE, CONFINEMENT

Downloads

  • (...).docx
    • full text
    • |
    • UGent only
    • |
    • Word
    • |
    • 8.35 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Houthaeve, Gaëlle, Ranhua Xiong, Joke Robijns, Bert Luyckx, Yasmine Beulque, Toon Brans, Coen Campsteijn, et al. 2018. “Targeted Perturbation of Nuclear Envelope Integrity with Vapor Nanobubble-mediated Photoporation.” Acs Nano 12 (8): 7791–7802.
APA
Houthaeve, G., Xiong, R., Robijns, J., Luyckx, B., Beulque, Y., Brans, T., Campsteijn, C., et al. (2018). Targeted perturbation of nuclear envelope integrity with vapor nanobubble-mediated photoporation. ACS NANO, 12(8), 7791–7802.
Vancouver
1.
Houthaeve G, Xiong R, Robijns J, Luyckx B, Beulque Y, Brans T, et al. Targeted perturbation of nuclear envelope integrity with vapor nanobubble-mediated photoporation. ACS NANO. 2018;12(8):7791–802.
MLA
Houthaeve, Gaëlle, Ranhua Xiong, Joke Robijns, et al. “Targeted Perturbation of Nuclear Envelope Integrity with Vapor Nanobubble-mediated Photoporation.” ACS NANO 12.8 (2018): 7791–7802. Print.
@article{8569526,
  abstract     = {The nuclear envelope (NE) has long been considered to dismantle only during mitosis. However, recent observations in cancer cells and laminopathy patient cells have revealed that the NE can also transiently rupture during interphase, thereby perturbing cellular homeostasis. Although NE ruptures are promoted by mechanical force and the loss of lamins, their stochastic nature and variable frequency preclude the study of their direct downstream consequences. We have developed a method based on vapor nanobubble-mediated photoporation that allows for deliberately inducing NE ruptures in a spatiotemporally controlled manner. Our method relies on wide-field laser illumination of perinuclear gold nanoparticles, resulting in the formation of short-lived vapor nanobubbles that inflict minute mechanical damage to the NE, thus creating small pores. We demonstrate that perinuclear localization of gold nanoparticles can be achieved after endocytic uptake or electroporation-facilitated delivery and that both strategies result in NE rupture upon laser irradiation. Furthermore, we prove that photoporation-induced nuclear ruptures are transient and recapitulate hallmarks of spontaneous NE ruptures that occur in A-type lamin-depleted cells. Finally, we show that the same approach can be used to promote influx of macromolecules that are too large to passively migrate through the NE. Thus, by providing unprecedented control over nuclear compartmentalization, nuclear photoporation offers a powerful tool for both fundamental cell biology research and drug delivery applications.},
  author       = {Houthaeve, Ga{\"e}lle and Xiong, Ranhua and Robijns, Joke and Luyckx, Bert and Beulque, Yasmine and Brans, Toon and Campsteijn, Coen and Samal, Sangram Keshari and Stremersch, Stephan and De Smedt, Stefaan and Braeckmans, Kevin and De Vos, Winnok},
  issn         = {1936-0851},
  journal      = {ACS NANO},
  language     = {eng},
  number       = {8},
  pages        = {7791--7802},
  title        = {Targeted perturbation of nuclear envelope integrity with vapor nanobubble-mediated photoporation},
  url          = {http://dx.doi.org/10.1021/acsnano.8b01860},
  volume       = {12},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: