Advanced search
1 file | 925.57 KB Add to list

Burkholderia insecticola sp. nov., a gut symbiotic bacterium of the bean bug Riptortus pedestris

Author
Organization
Abstract
A Gram-negative, aerobic, rod-shaped, non-spore-forming, motile bacterium, designated strain RPE64(T), was isolated from the gut symbiotic organ of the bean bug Riptortus pedestris, collected in Tsukuba, Japan, in 2007. 16S rRNA gene sequencing showed that this strain belongs to the Burkholderia glathei Glade, exhibiting the highest sequence similarity to Burkholderia peredens LMG 29314(T) (100%), Burkholderia turbans LMG 29316(T) (99.52%) and Burkholderia ptereochthonis LMG 29326(T) (99.04 %). Phylogenomic analyses based on 107 single-copy core genes and Genome BLAST Distance Phylogeny confirmed B. peredens LMG 29314(T), B. ptereochthonis LMG 29326(T) and several uncultivated, endophytic Burkholderia species as its nearest phylogenetic neighbours. Digital DNA-DNA hybridization experiments unambiguously demonstrated that strain RPE64(T) represents a novel species in this lineage. The G+C content of its genome was 63.2 mol%. The isoprenoid quinone was ubiquinone 8 and the predominant fatty acid components were C-16:0, C-18:1 omega 7c and C-17:0 cyclo. The absence of nitrate reduction and the capacity to grow at pH 8 clearly differentiated strain RPE64(T) from related Burkholderia species. Based on these genotypic and phenotypic characteristics, strain RPE64(T) is classified as representing a novel species of the genus Burkholderia, for which the name Burkholderia insecticola sp. nov. is proposed. The type strain is RPE64(T) (=NCIMB 15023(T)=JCM 31142(T)).
Keywords
Burkholderia insecticola, Burkholderia glathei clade, gut symbiont, Riptortus pedestris, GRIMMIAE, PROPOSAL

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 925.57 KB

Citation

Please use this url to cite or link to this publication:

MLA
Takeshita, Kazutaka, Hideyuki Tamaki, Tsubasa Ohbayashi, et al. “Burkholderia Insecticola Sp. Nov., a Gut Symbiotic Bacterium of the Bean Bug Riptortus Pedestris.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 68.7 (2018): 2370–2374. Print.
APA
Takeshita, Kazutaka, Tamaki, H., Ohbayashi, T., Meng, X.-Y., Sone, T., Mitani, Y., Peeters, C., et al. (2018). Burkholderia insecticola sp. nov., a gut symbiotic bacterium of the bean bug Riptortus pedestris. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 68(7), 2370–2374.
Chicago author-date
Takeshita, Kazutaka, Hideyuki Tamaki, Tsubasa Ohbayashi, Xian-Ying Meng, Teruo Sone, Yasuo Mitani, Charlotte Peeters, Yoshitomo Kikuchi, and Peter Vandamme. 2018. “Burkholderia Insecticola Sp. Nov., a Gut Symbiotic Bacterium of the Bean Bug Riptortus Pedestris.” International Journal of Systematic and Evolutionary Microbiology 68 (7): 2370–2374.
Chicago author-date (all authors)
Takeshita, Kazutaka, Hideyuki Tamaki, Tsubasa Ohbayashi, Xian-Ying Meng, Teruo Sone, Yasuo Mitani, Charlotte Peeters, Yoshitomo Kikuchi, and Peter Vandamme. 2018. “Burkholderia Insecticola Sp. Nov., a Gut Symbiotic Bacterium of the Bean Bug Riptortus Pedestris.” International Journal of Systematic and Evolutionary Microbiology 68 (7): 2370–2374.
Vancouver
1.
Takeshita K, Tamaki H, Ohbayashi T, Meng X-Y, Sone T, Mitani Y, et al. Burkholderia insecticola sp. nov., a gut symbiotic bacterium of the bean bug Riptortus pedestris. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2018;68(7):2370–4.
IEEE
[1]
K. Takeshita et al., “Burkholderia insecticola sp. nov., a gut symbiotic bacterium of the bean bug Riptortus pedestris,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 68, no. 7, pp. 2370–2374, 2018.
@article{8567878,
  abstract     = {A Gram-negative, aerobic, rod-shaped, non-spore-forming, motile bacterium, designated strain RPE64(T), was isolated from the gut symbiotic organ of the bean bug Riptortus pedestris, collected in Tsukuba, Japan, in 2007. 16S rRNA gene sequencing showed that this strain belongs to the Burkholderia glathei Glade, exhibiting the highest sequence similarity to Burkholderia peredens LMG 29314(T) (100%), Burkholderia turbans LMG 29316(T) (99.52%) and Burkholderia ptereochthonis LMG 29326(T) (99.04 %). Phylogenomic analyses based on 107 single-copy core genes and Genome BLAST Distance Phylogeny confirmed B. peredens LMG 29314(T), B. ptereochthonis LMG 29326(T) and several uncultivated, endophytic Burkholderia species as its nearest phylogenetic neighbours. Digital DNA-DNA hybridization experiments unambiguously demonstrated that strain RPE64(T) represents a novel species in this lineage. The G+C content of its genome was 63.2 mol%. The isoprenoid quinone was ubiquinone 8 and the predominant fatty acid components were C-16:0, C-18:1 omega 7c and C-17:0 cyclo. The absence of nitrate reduction and the capacity to grow at pH 8 clearly differentiated strain RPE64(T) from related Burkholderia species. Based on these genotypic and phenotypic characteristics, strain RPE64(T) is classified as representing a novel species of the genus Burkholderia, for which the name Burkholderia insecticola sp. nov. is proposed. The type strain is RPE64(T) (=NCIMB 15023(T)=JCM 31142(T)).},
  author       = {Takeshita, Kazutaka and Tamaki, Hideyuki and Ohbayashi, Tsubasa and Meng, Xian-Ying and Sone, Teruo and Mitani, Yasuo and Peeters, Charlotte and Kikuchi, Yoshitomo and Vandamme, Peter},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keywords     = {Burkholderia insecticola,Burkholderia glathei clade,gut symbiont,Riptortus pedestris,GRIMMIAE,PROPOSAL},
  language     = {eng},
  number       = {7},
  pages        = {2370--2374},
  title        = {Burkholderia insecticola sp. nov., a gut symbiotic bacterium of the bean bug Riptortus pedestris},
  url          = {http://dx.doi.org/10.1099/ijsem.0.002848},
  volume       = {68},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: