Advanced search
1 file | 551.72 KB

Isotope fractionation in biogas allows direct microbial community stability monitoring in anaerobic digestion

Jo De Vrieze (UGent) , Michiel De Waele, Pascal Boeckx (UGent) and Nico Boon (UGent)
(2018) ENVIRONMENTAL SCIENCE & TECHNOLOGY. 52(11). p.6704-6713
Author
Organization
Abstract
Process monitoring of anaerobic digestion is typically based on operational parameters, such as pH and volatile fatty acid concentration, that are lagging on actual microbial community performance. In this study, C-13 isotope fractionation in CH4 and CO2 in the biogas was used to monitor process stability of anaerobic digestion in response to salt stress. A gradual and pulsed increase in salt concentration resulted in a decrease in methane production. No clear shift in delta(CH4)-C-13 was observed in response to the gradual increase in salt concentration, and delta(CO2)-C-13 of the biogas showed only a clear shift after process failure, compared with the control. In contrast, both delta(CH4)-C-13 and delta(CO2)-C-13 in the biogas changed in response to the pulsed increase in salt concentration. This change preceded the decrease in methane production. A significantly different bacterial and archaeal community profile was observed between the DNA and RNA level, which was also reflected in a different relation with the delta(CH4)-C-13 and delta(CO2)-C-13 values. This shows that isotope fractionation in the biogas can predict process stability in anaerobic digestion, as it directly reflects shifts in the total and active microbial community, yet, due to its temporal character, further validation is needed.
Keywords
STABLE CARBON ISOTOPES, VOLATILE FATTY-ACIDS, METHANOGENIC PATHWAYS, PROCESS FAILURE, ACETATE OXIDATION, CO-DIGESTION, INHIBITION, PARAMETERS, METHANE, REDUNDANCY

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 551.72 KB

Citation

Please use this url to cite or link to this publication:

Chicago
De Vrieze, Jo, Michiel De Waele, Pascal Boeckx, and Nico Boon. 2018. “Isotope Fractionation in Biogas Allows Direct Microbial Community Stability Monitoring in Anaerobic Digestion.” Environmental Science & Technology 52 (11): 6704–6713.
APA
De Vrieze, Jo, De Waele, M., Boeckx, P., & Boon, N. (2018). Isotope fractionation in biogas allows direct microbial community stability monitoring in anaerobic digestion. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 52(11), 6704–6713.
Vancouver
1.
De Vrieze J, De Waele M, Boeckx P, Boon N. Isotope fractionation in biogas allows direct microbial community stability monitoring in anaerobic digestion. ENVIRONMENTAL SCIENCE & TECHNOLOGY. 2018;52(11):6704–13.
MLA
De Vrieze, Jo, Michiel De Waele, Pascal Boeckx, et al. “Isotope Fractionation in Biogas Allows Direct Microbial Community Stability Monitoring in Anaerobic Digestion.” ENVIRONMENTAL SCIENCE & TECHNOLOGY 52.11 (2018): 6704–6713. Print.
@article{8567362,
  abstract     = {Process monitoring of anaerobic digestion is typically based on operational parameters, such as pH and volatile fatty acid concentration, that are lagging on actual microbial community performance. In this study, C-13 isotope fractionation in CH4 and CO2 in the biogas was used to monitor process stability of anaerobic digestion in response to salt stress. A gradual and pulsed increase in salt concentration resulted in a decrease in methane production. No clear shift in delta(CH4)-C-13 was observed in response to the gradual increase in salt concentration, and delta(CO2)-C-13 of the biogas showed only a clear shift after process failure, compared with the control. In contrast, both delta(CH4)-C-13 and delta(CO2)-C-13 in the biogas changed in response to the pulsed increase in salt concentration. This change preceded the decrease in methane production. A significantly different bacterial and archaeal community profile was observed between the DNA and RNA level, which was also reflected in a different relation with the delta(CH4)-C-13 and delta(CO2)-C-13 values. This shows that isotope fractionation in the biogas can predict process stability in anaerobic digestion, as it directly reflects shifts in the total and active microbial community, yet, due to its temporal character, further validation is needed.},
  author       = {De Vrieze, Jo and De Waele, Michiel and Boeckx, Pascal and Boon, Nico},
  issn         = {0013-936X},
  journal      = {ENVIRONMENTAL SCIENCE \& TECHNOLOGY},
  keyword      = {STABLE CARBON ISOTOPES,VOLATILE FATTY-ACIDS,METHANOGENIC PATHWAYS,PROCESS FAILURE,ACETATE OXIDATION,CO-DIGESTION,INHIBITION,PARAMETERS,METHANE,REDUNDANCY},
  language     = {eng},
  number       = {11},
  pages        = {6704--6713},
  title        = {Isotope fractionation in biogas allows direct microbial community stability monitoring in anaerobic digestion},
  url          = {http://dx.doi.org/10.1021/acs.est.8b00723},
  volume       = {52},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: