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Influence of growth media components on the antibacterial effect of silver ions on Bacillus subtilis in a liquid growth medium

Ilse De Leersnyder (UGent) , Leen De Gelder (UGent) , Isabel Van Driessche (UGent) and Pieter Vermeir (UGent)
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Abstract
Numerous studies have investigated the antibacterial effect of both silver ions and silver nanomaterials on a large diversity of environmentally and clinically relevant bacteria. However, contradictory results are reported in which inhibition concentrations were varying by a 10-fold. This study investigated whether this variance in results could be attributed to the difference in experimental conditions, especially the microbial growth medium. B. subtilis was exposed to 500 mu g L-1 Ag+ in liquid growth media with different concentrations of some commonly used media components: tryptone, yeast extract, Cl-, and S2-. The toxic effect was investigated by means of three complementary analysis techniques: (i) analyzing the growth curves obtained by optical density measurements, (ii) using flow cytometry, and (iii) by transmission electron microscopy. The silver ion toxicity towards B. subtilis decreased as more tryptone, yeast extract, or S2- was present. This study demonstrates that the medium composition, rarely acknowledged as an important experimental factor in bacterial toxicity studies, has a profound impact on the observed silver toxicity towards B. subtilis.
Keywords
ESCHERICHIA-COLI, STAPHYLOCOCCUS-AUREUS, NANOPARTICLES, TOXICITY, BACTERIA, STABILITY, IMPACT, RELEASE

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Chicago
De Leersnyder, Ilse, Leen De Gelder, Isabel Van Driessche, and Pieter Vermeir. 2018. “Influence of Growth Media Components on the Antibacterial Effect of Silver Ions on Bacillus Subtilis in a Liquid Growth Medium.” Scientific Reports 8.
APA
De Leersnyder, I., De Gelder, L., Van Driessche, I., & Vermeir, P. (2018). Influence of growth media components on the antibacterial effect of silver ions on Bacillus subtilis in a liquid growth medium. SCIENTIFIC REPORTS, 8.
Vancouver
1.
De Leersnyder I, De Gelder L, Van Driessche I, Vermeir P. Influence of growth media components on the antibacterial effect of silver ions on Bacillus subtilis in a liquid growth medium. SCIENTIFIC REPORTS. 2018;8.
MLA
De Leersnyder, Ilse, Leen De Gelder, Isabel Van Driessche, et al. “Influence of Growth Media Components on the Antibacterial Effect of Silver Ions on Bacillus Subtilis in a Liquid Growth Medium.” SCIENTIFIC REPORTS 8 (2018): n. pag. Print.
@article{8567054,
  abstract     = {Numerous studies have investigated the antibacterial effect of both silver ions and silver nanomaterials on a large diversity of environmentally and clinically relevant bacteria. However, contradictory results are reported in which inhibition concentrations were varying by a 10-fold. This study investigated whether this variance in results could be attributed to the difference in experimental conditions, especially the microbial growth medium. B. subtilis was exposed to 500 mu g L-1 Ag+ in liquid growth media with different concentrations of some commonly used media components: tryptone, yeast extract, Cl-, and S2-. The toxic effect was investigated by means of three complementary analysis techniques: (i) analyzing the growth curves obtained by optical density measurements, (ii) using flow cytometry, and (iii) by transmission electron microscopy. The silver ion toxicity towards B. subtilis decreased as more tryptone, yeast extract, or S2- was present. This study demonstrates that the medium composition, rarely acknowledged as an important experimental factor in bacterial toxicity studies, has a profound impact on the observed silver toxicity towards B. subtilis.},
  articleno    = {9325},
  author       = {De Leersnyder, Ilse and De Gelder, Leen and Van Driessche, Isabel and Vermeir, Pieter},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {ESCHERICHIA-COLI,STAPHYLOCOCCUS-AUREUS,NANOPARTICLES,TOXICITY,BACTERIA,STABILITY,IMPACT,RELEASE},
  language     = {eng},
  pages        = {10},
  title        = {Influence of growth media components on the antibacterial effect of silver ions on Bacillus subtilis in a liquid growth medium},
  url          = {http://dx.doi.org/10.1038/s41598-018-27540-9},
  volume       = {8},
  year         = {2018},
}

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