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Phloroglucinol-mediated Hsp70 production in crustaceans : protection against Vibrio parahaemolyticus in Artemia franciscana and Macrobrachium rosenbergii

Vikash Kumar (UGent) , Sri Kartik Baruah (UGent) , Viet Dung Nguyen (UGent) , Guy Smagghe (UGent) , Els Vossen (UGent) and Peter Bossier (UGent)
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Abstract
The halophilic aquatic bacterium, Vibrio parahaemolyticus, is an important aquatic pathogen, also capable of causing acute hepatopancreatic necrosis disease (AHPND) in shrimp resulting in significant economic losses. Therefore, there is an urgent need to develop anti-infective strategies to control AHPND. The gnotobiotic Artemia model is used to establish whether a phenolic compound phloroglucinol is effective against the AHPND strain V. parahaemolyticus MO904. We found that pretreatment with phloroglucinol, at an optimum concentration (30 mu M), protects axenic brine shrimp larvae against V. parahaemolyticus infection and induced heat shock protein 70 (Hsp70) production (twofolds or more) as compared with the control. We further demonstrated that the Vibrio-protective effect of phloroglucinol was caused by its prooxidant effect and is linked to the induction of Hsp70. In addition, RNA interference confirms that phloroglucinol-induced Hsp70 mediates the survival of brine shrimp larvae against V. parahaemolyticus infection. The study was validated in xenic Artemia model and in a Macrobrachium rosenbergii system. Pretreatment of xenic brine shrimp larvae (30 mu M) and Macrobrachium larvae (5 mu M) with phloroglucinol increases the survival of xenic brine shrimp and Macrobrachium larvae against subsequent V. parahaemolyticus challenge. Taken together, our study provides substantial evidence that the prooxidant activity of phloroglucinol induces Hsp70 production protecting brine shrimp, A. franciscana, and freshwater shrimp, M. rosenbergii, against the AHPND V. parahaemolyticus strain MO904. Probably, phloroglucinol treatment might become part of a holistic strategy to control AHPND in shrimp.
Keywords
gnotobiotic Artemia, Vibrio parahaemolyticus, phloroglucinol, heat shock protein 70, Macrobrachium rosenbergii, NECROSIS DISEASE AHPND, HEAT-SHOCK PROTEINS, CAMPBELLII CHALLENGE, HARVEYI INFECTION, CAUSATIVE AGENT, BRINE SHRIMP, RESISTANCE, PERFORMANCE, AQUACULTURE, TOLERANCE

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Chicago
Kumar, Vikash, Sri Kartik Baruah, Viet Dung Nguyen, Guy Smagghe, Els Vossen, and Peter Bossier. 2018. “Phloroglucinol-mediated Hsp70 Production in Crustaceans : Protection Against Vibrio Parahaemolyticus in Artemia Franciscana and Macrobrachium Rosenbergii.” Frontiers in Immunology 9.
APA
Kumar, V., Baruah, S. K., Nguyen, V. D., Smagghe, G., Vossen, E., & Bossier, P. (2018). Phloroglucinol-mediated Hsp70 production in crustaceans : protection against Vibrio parahaemolyticus in Artemia franciscana and Macrobrachium rosenbergii. FRONTIERS IN IMMUNOLOGY, 9.
Vancouver
1.
Kumar V, Baruah SK, Nguyen VD, Smagghe G, Vossen E, Bossier P. Phloroglucinol-mediated Hsp70 production in crustaceans : protection against Vibrio parahaemolyticus in Artemia franciscana and Macrobrachium rosenbergii. FRONTIERS IN IMMUNOLOGY. 2018;9.
MLA
Kumar, Vikash et al. “Phloroglucinol-mediated Hsp70 Production in Crustaceans : Protection Against Vibrio Parahaemolyticus in Artemia Franciscana and Macrobrachium Rosenbergii.” FRONTIERS IN IMMUNOLOGY 9 (2018): n. pag. Print.
@article{8565733,
  abstract     = {The halophilic aquatic bacterium, Vibrio parahaemolyticus, is an important aquatic pathogen, also capable of causing acute hepatopancreatic necrosis disease (AHPND) in shrimp resulting in significant economic losses. Therefore, there is an urgent need to develop anti-infective strategies to control AHPND. The gnotobiotic Artemia model is used to establish whether a phenolic compound phloroglucinol is effective against the AHPND strain V. parahaemolyticus MO904. We found that pretreatment with phloroglucinol, at an optimum concentration (30 mu M), protects axenic brine shrimp larvae against V. parahaemolyticus infection and induced heat shock protein 70 (Hsp70) production (twofolds or more) as compared with the control. We further demonstrated that the Vibrio-protective effect of phloroglucinol was caused by its prooxidant effect and is linked to the induction of Hsp70. In addition, RNA interference confirms that phloroglucinol-induced Hsp70 mediates the survival of brine shrimp larvae against V. parahaemolyticus infection. The study was validated in xenic Artemia model and in a Macrobrachium rosenbergii system. Pretreatment of xenic brine shrimp larvae (30 mu M) and Macrobrachium larvae (5 mu M) with phloroglucinol increases the survival of xenic brine shrimp and Macrobrachium larvae against subsequent V. parahaemolyticus challenge. Taken together, our study provides substantial evidence that the prooxidant activity of phloroglucinol induces Hsp70 production protecting brine shrimp, A. franciscana, and freshwater shrimp, M. rosenbergii, against the AHPND V. parahaemolyticus strain MO904. Probably, phloroglucinol treatment might become part of a holistic strategy to control AHPND in shrimp.},
  articleno    = {1091},
  author       = {Kumar, Vikash and Baruah, Sri Kartik and Nguyen, Viet Dung and Smagghe, Guy and Vossen, Els and Bossier, Peter},
  issn         = {1664-3224},
  journal      = {FRONTIERS IN IMMUNOLOGY},
  keywords     = {gnotobiotic Artemia,Vibrio parahaemolyticus,phloroglucinol,heat shock protein 70,Macrobrachium rosenbergii,NECROSIS DISEASE AHPND,HEAT-SHOCK PROTEINS,CAMPBELLII CHALLENGE,HARVEYI INFECTION,CAUSATIVE AGENT,BRINE SHRIMP,RESISTANCE,PERFORMANCE,AQUACULTURE,TOLERANCE},
  language     = {eng},
  pages        = {16},
  title        = {Phloroglucinol-mediated Hsp70 production in crustaceans : protection against Vibrio parahaemolyticus in Artemia franciscana and Macrobrachium rosenbergii},
  url          = {http://dx.doi.org/10.3389/fimmu.2018.01091},
  volume       = {9},
  year         = {2018},
}

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