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Effect of ethanol and butanol on autotrophic growth of model homoacetogens

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Abstract
Research efforts aimed at increasing ethanol and butanol productivity from syngas are currently gaining attention. For most model carboxydotrophic bacteria, production rates, yields and maximum product titres have been studied in detail, but little is known on alcohol toxicity in these bacteria. The aim of this work was to investigate the inhibitory effects of ethanol and butanol on the growth of Clostridium ljungdahlii PETC, C. carboxidiwrans P7, and 'Butyribacterium methylotrophicum DSM3468'. Experiments to determine inhibitory effects due to product accumulation were carried out using a synthetic mixture of CO:CO2:H-2 as a substrate. These conditions were chosen to mimic gaseous effluents of biomass and waste gasification plants. Inhibition effects were recorded as changes in growth parameters. No significant inhibition was observed for ethanol at concentrations below 15 g/L. The three species exhibited higher sensitivity to butanol. Half inhibition constants for butanol could be estimated for P7 (IC50 = 4.12 g/L), DSM3468 (IC50 = 1.79 g/L), and PETC IC50 = 9.75 g/L). In conclusion, at least for the tested strains, alcohol toxicity is not an immediate handicap for increasing alcohol production of the tested homoacetogenic strains.
Keywords
alcohol toxicity, syngas, Clostridia, CO2 utilisation, IC50, CLOSTRIDIUM-LJUNGDAHLII, SYNTHESIS GAS, INHIBITION-KINETICS, MEMBRANE-FLUIDITY, LIPID-COMPOSITION, CARBON-MONOXIDE, SP-NOV, FERMENTATION, SYNGAS, ACETOBUTYLICUM

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MLA
Ramió-Pujol, Sara, et al. “Effect of Ethanol and Butanol on Autotrophic Growth of Model Homoacetogens.” FEMS MICROBIOLOGY LETTERS, vol. 365, no. 10, 2018.
APA
Ramió-Pujol, S., Ganigué, R., Bañeras, L., & Colprim, J. (2018). Effect of ethanol and butanol on autotrophic growth of model homoacetogens. FEMS MICROBIOLOGY LETTERS, 365(10).
Chicago author-date
Ramió-Pujol, Sara, Ramon Ganigué, Lluís Bañeras, and Jesús Colprim. 2018. “Effect of Ethanol and Butanol on Autotrophic Growth of Model Homoacetogens.” FEMS MICROBIOLOGY LETTERS 365 (10).
Chicago author-date (all authors)
Ramió-Pujol, Sara, Ramon Ganigué, Lluís Bañeras, and Jesús Colprim. 2018. “Effect of Ethanol and Butanol on Autotrophic Growth of Model Homoacetogens.” FEMS MICROBIOLOGY LETTERS 365 (10).
Vancouver
1.
Ramió-Pujol S, Ganigué R, Bañeras L, Colprim J. Effect of ethanol and butanol on autotrophic growth of model homoacetogens. FEMS MICROBIOLOGY LETTERS. 2018;365(10).
IEEE
[1]
S. Ramió-Pujol, R. Ganigué, L. Bañeras, and J. Colprim, “Effect of ethanol and butanol on autotrophic growth of model homoacetogens,” FEMS MICROBIOLOGY LETTERS, vol. 365, no. 10, 2018.
@article{8565724,
  abstract     = {Research efforts aimed at increasing ethanol and butanol productivity from syngas are currently gaining attention. For most model carboxydotrophic bacteria, production rates, yields and maximum product titres have been studied in detail, but little is known on alcohol toxicity in these bacteria. The aim of this work was to investigate the inhibitory effects of ethanol and butanol on the growth of Clostridium ljungdahlii PETC, C. carboxidiwrans P7, and 'Butyribacterium methylotrophicum DSM3468'. Experiments to determine inhibitory effects due to product accumulation were carried out using a synthetic mixture of CO:CO2:H-2 as a substrate. These conditions were chosen to mimic gaseous effluents of biomass and waste gasification plants. Inhibition effects were recorded as changes in growth parameters. No significant inhibition was observed for ethanol at concentrations below 15 g/L. The three species exhibited higher sensitivity to butanol. Half inhibition constants for butanol could be estimated for P7 (IC50 = 4.12 g/L), DSM3468 (IC50 = 1.79 g/L), and PETC IC50 = 9.75 g/L). In conclusion, at least for the tested strains, alcohol toxicity is not an immediate handicap for increasing alcohol production of the tested homoacetogenic strains.},
  articleno    = {fny084},
  author       = {Ramió-Pujol, Sara and Ganigué, Ramon and Bañeras, Lluís and Colprim, Jesús},
  issn         = {0378-1097},
  journal      = {FEMS MICROBIOLOGY LETTERS},
  keywords     = {alcohol toxicity,syngas,Clostridia,CO2 utilisation,IC50,CLOSTRIDIUM-LJUNGDAHLII,SYNTHESIS GAS,INHIBITION-KINETICS,MEMBRANE-FLUIDITY,LIPID-COMPOSITION,CARBON-MONOXIDE,SP-NOV,FERMENTATION,SYNGAS,ACETOBUTYLICUM},
  language     = {eng},
  number       = {10},
  pages        = {4},
  title        = {Effect of ethanol and butanol on autotrophic growth of model homoacetogens},
  url          = {http://dx.doi.org/10.1093/femsle/fny084},
  volume       = {365},
  year         = {2018},
}

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