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Chitosan functionalized poly-ε-caprolactone electrospun fibers and 3D printed scaffolds as antibacterial materials for tissue engineering applications

(2018) CARBOHYDRATE POLYMERS. 191. p.127-135
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Abstract
Tissue engineering (TE) approaches often employ polymer-based scaffolds to provide support with a view to the improved regeneration of damaged tissues. The aim of this research was to develop a surface modification method for introducing chitosan as an antibacterial agent in both electrospun membranes and 3D printed poly-epsilon-caprolactone (PCL) scaffolds. The scaffolds were functionalized by grafting methacrylic acid N-hydroxysuccinimide ester (NHSMA) onto the surface after Ar-plasma/air activation. Subsequently, the newly-introduced NHS groups were used to couple with chitosan of various molecular weights (Mw). High Mw chitosan exhibited a better coverage of the surface as indicated by the higher N% detected by X-ray photoelectron spectroscopy (XPS) and the observations with either scanning electron microscopy (SEM)(for fibers) or Coomassie blue staining (for 3D-printed scaffolds). A lactate dehydrogenase assay (LDH) using L929 fibroblasts demonstrated the cell-adhesion and cell-viability capacity of the modified samples. The antibacterial properties against S. aureus ATCC 6538 and S. epidermidis ET13 revealed a slower bacterial growth rate on the surface of the chitosan modified scaffolds, regardless the chitosan Mw.
Keywords
RAPID PROTOTYPING TECHNIQUES, HUMAN ENDOTHELIAL-CELLS, MESENCHYMAL, STEM-CELLS, SURFACE MODIFICATION, POLYCAPROLACTONE SCAFFOLDS, ANTIMICROBIAL PROPERTIES, NANOFIBROUS MEMBRANES, COMPOSITE SCAFFOLDS, PLASMA TREATMENT, DRUG-DELIVERY, Chitosan, Surface functionalization, Scaffolds, Poly-epsilon-caprolactone, Antibacterial

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Citation

Please use this url to cite or link to this publication:

MLA
Tardajos, Myriam G et al. “Chitosan Functionalized Poly-ε-caprolactone Electrospun Fibers and 3D Printed Scaffolds as Antibacterial Materials for Tissue Engineering Applications.” CARBOHYDRATE POLYMERS 191 (2018): 127–135. Print.
APA
Tardajos, M. G., Cama, G., Dash, M., Misseeuw, L., Gheysens, T., Gorzelanny, C., Coenye, T., et al. (2018). Chitosan functionalized poly-ε-caprolactone electrospun fibers and 3D printed scaffolds as antibacterial materials for tissue engineering applications. CARBOHYDRATE POLYMERS, 191, 127–135.
Chicago author-date
Tardajos, Myriam G, Giuseppe Cama, Mamoni Dash, Lara Misseeuw, Tom Gheysens, Christian Gorzelanny, Tom Coenye, and Peter Dubruel. 2018. “Chitosan Functionalized Poly-ε-caprolactone Electrospun Fibers and 3D Printed Scaffolds as Antibacterial Materials for Tissue Engineering Applications.” Carbohydrate Polymers 191: 127–135.
Chicago author-date (all authors)
Tardajos, Myriam G, Giuseppe Cama, Mamoni Dash, Lara Misseeuw, Tom Gheysens, Christian Gorzelanny, Tom Coenye, and Peter Dubruel. 2018. “Chitosan Functionalized Poly-ε-caprolactone Electrospun Fibers and 3D Printed Scaffolds as Antibacterial Materials for Tissue Engineering Applications.” Carbohydrate Polymers 191: 127–135.
Vancouver
1.
Tardajos MG, Cama G, Dash M, Misseeuw L, Gheysens T, Gorzelanny C, et al. Chitosan functionalized poly-ε-caprolactone electrospun fibers and 3D printed scaffolds as antibacterial materials for tissue engineering applications. CARBOHYDRATE POLYMERS. 2018;191:127–35.
IEEE
[1]
M. G. Tardajos et al., “Chitosan functionalized poly-ε-caprolactone electrospun fibers and 3D printed scaffolds as antibacterial materials for tissue engineering applications,” CARBOHYDRATE POLYMERS, vol. 191, pp. 127–135, 2018.
@article{8565432,
  abstract     = {Tissue engineering (TE) approaches often employ polymer-based scaffolds to provide support with a view to the improved regeneration of damaged tissues. The aim of this research was to develop a surface modification method for introducing chitosan as an antibacterial agent in both electrospun membranes and 3D printed poly-epsilon-caprolactone (PCL) scaffolds. The scaffolds were functionalized by grafting methacrylic acid N-hydroxysuccinimide ester (NHSMA) onto the surface after Ar-plasma/air activation. Subsequently, the newly-introduced NHS groups were used to couple with chitosan of various molecular weights (Mw). High Mw chitosan exhibited a better coverage of the surface as indicated by the higher N% detected by X-ray photoelectron spectroscopy (XPS) and the observations with either scanning electron microscopy (SEM)(for fibers) or Coomassie blue staining (for 3D-printed scaffolds). A lactate dehydrogenase assay (LDH) using L929 fibroblasts demonstrated the cell-adhesion and cell-viability capacity of the modified samples. The antibacterial properties against S. aureus ATCC 6538 and S. epidermidis ET13 revealed a slower bacterial growth rate on the surface of the chitosan modified scaffolds, regardless the chitosan Mw.},
  author       = {Tardajos, Myriam G and Cama, Giuseppe and Dash, Mamoni and Misseeuw, Lara and Gheysens, Tom and Gorzelanny, Christian and Coenye, Tom and Dubruel, Peter},
  issn         = {0144-8617},
  journal      = {CARBOHYDRATE POLYMERS},
  keywords     = {RAPID PROTOTYPING TECHNIQUES,HUMAN ENDOTHELIAL-CELLS,MESENCHYMAL,STEM-CELLS,SURFACE MODIFICATION,POLYCAPROLACTONE SCAFFOLDS,ANTIMICROBIAL PROPERTIES,NANOFIBROUS MEMBRANES,COMPOSITE SCAFFOLDS,PLASMA TREATMENT,DRUG-DELIVERY,Chitosan,Surface functionalization,Scaffolds,Poly-epsilon-caprolactone,Antibacterial},
  language     = {eng},
  pages        = {127--135},
  title        = {Chitosan functionalized poly-ε-caprolactone electrospun fibers and 3D printed scaffolds as antibacterial materials for tissue engineering applications},
  url          = {http://dx.doi.org/10.1016/j.carbpol.2018.02.060},
  volume       = {191},
  year         = {2018},
}

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