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Chitosan functionalized poly-ε-caprolactone electrospun fibers and 3D printed scaffolds as antibacterial materials for tissue engineering applications

(2018) CARBOHYDRATE POLYMERS. 191. p.127-135
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Abstract
Tissue engineering (TE) approaches often employ polymer-based scaffolds to provide support with a view to the improved regeneration of damaged tissues. The aim of this research was to develop a surface modification method for introducing chitosan as an antibacterial agent in both electrospun membranes and 3D printed poly-epsilon-caprolactone (PCL) scaffolds. The scaffolds were functionalized by grafting methacrylic acid N-hydroxysuccinimide ester (NHSMA) onto the surface after Ar-plasma/air activation. Subsequently, the newly-introduced NHS groups were used to couple with chitosan of various molecular weights (Mw). High Mw chitosan exhibited a better coverage of the surface as indicated by the higher N% detected by X-ray photoelectron spectroscopy (XPS) and the observations with either scanning electron microscopy (SEM)(for fibers) or Coomassie blue staining (for 3D-printed scaffolds). A lactate dehydrogenase assay (LDH) using L929 fibroblasts demonstrated the cell-adhesion and cell-viability capacity of the modified samples. The antibacterial properties against S. aureus ATCC 6538 and S. epidermidis ET13 revealed a slower bacterial growth rate on the surface of the chitosan modified scaffolds, regardless the chitosan Mw.
Keywords
RAPID PROTOTYPING TECHNIQUES, HUMAN ENDOTHELIAL-CELLS, MESENCHYMAL, STEM-CELLS, SURFACE MODIFICATION, POLYCAPROLACTONE SCAFFOLDS, ANTIMICROBIAL PROPERTIES, NANOFIBROUS MEMBRANES, COMPOSITE SCAFFOLDS, PLASMA TREATMENT, DRUG-DELIVERY, Chitosan, Surface functionalization, Scaffolds, Poly-epsilon-caprolactone, Antibacterial

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Citation

Please use this url to cite or link to this publication:

Chicago
Tardajos, Myriam G, Giuseppe Cama, Mamoni Dash, Lara Misseeuw, Tom Gheysens, Christian Gorzelanny, Tom Coenye, and Peter Dubruel. 2018. “Chitosan Functionalized Poly-ε-caprolactone Electrospun Fibers and 3D Printed Scaffolds as Antibacterial Materials for Tissue Engineering Applications.” Carbohydrate Polymers 191: 127–135.
APA
Tardajos, M. G., Cama, G., Dash, M., Misseeuw, L., Gheysens, T., Gorzelanny, C., Coenye, T., et al. (2018). Chitosan functionalized poly-ε-caprolactone electrospun fibers and 3D printed scaffolds as antibacterial materials for tissue engineering applications. CARBOHYDRATE POLYMERS, 191, 127–135.
Vancouver
1.
Tardajos MG, Cama G, Dash M, Misseeuw L, Gheysens T, Gorzelanny C, et al. Chitosan functionalized poly-ε-caprolactone electrospun fibers and 3D printed scaffolds as antibacterial materials for tissue engineering applications. CARBOHYDRATE POLYMERS. 2018;191:127–35.
MLA
Tardajos, Myriam G et al. “Chitosan Functionalized Poly-ε-caprolactone Electrospun Fibers and 3D Printed Scaffolds as Antibacterial Materials for Tissue Engineering Applications.” CARBOHYDRATE POLYMERS 191 (2018): 127–135. Print.
@article{8565432,
  abstract     = {Tissue engineering (TE) approaches often employ polymer-based scaffolds to provide support with a view to the improved regeneration of damaged tissues. The aim of this research was to develop a surface modification method for introducing chitosan as an antibacterial agent in both electrospun membranes and 3D printed poly-epsilon-caprolactone (PCL) scaffolds. The scaffolds were functionalized by grafting methacrylic acid N-hydroxysuccinimide ester (NHSMA) onto the surface after Ar-plasma/air activation. Subsequently, the newly-introduced NHS groups were used to couple with chitosan of various molecular weights (Mw). High Mw chitosan exhibited a better coverage of the surface as indicated by the higher N\% detected by X-ray photoelectron spectroscopy (XPS) and the observations with either scanning electron microscopy (SEM)(for fibers) or Coomassie blue staining (for 3D-printed scaffolds). A lactate dehydrogenase assay (LDH) using L929 fibroblasts demonstrated the cell-adhesion and cell-viability capacity of the modified samples. The antibacterial properties against S. aureus ATCC 6538 and S. epidermidis ET13 revealed a slower bacterial growth rate on the surface of the chitosan modified scaffolds, regardless the chitosan Mw.},
  author       = {Tardajos, Myriam G and Cama, Giuseppe and Dash, Mamoni and Misseeuw, Lara and Gheysens, Tom and Gorzelanny, Christian and Coenye, Tom and Dubruel, Peter},
  issn         = {0144-8617},
  journal      = {CARBOHYDRATE POLYMERS},
  language     = {eng},
  pages        = {127--135},
  title        = {Chitosan functionalized poly-\ensuremath{\epsilon}-caprolactone electrospun fibers and 3D printed scaffolds as antibacterial materials for tissue engineering applications},
  url          = {http://dx.doi.org/10.1016/j.carbpol.2018.02.060},
  volume       = {191},
  year         = {2018},
}

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