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Comparison of three commercially available buffy coat pooling sets for the preparation of platelet concentrates

(2018) VOX SANGUINIS. 113(6). p.555-561
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Abstract
Background: A disposable set for platelet concentrate (PC) preparation by the buffy coat method allows pooling of buffy coats, centrifugation and cell separation with in-line leucocyte filtration. This study compares three commercially available pooling sets in combination with INTERCEPT pathogen inactivation (PI). Materials and methods: Sets for pooling of buffy coats were from Fresenius Kabi (FRE), Macopharma (MAC) and Terumo BCT (TER). Platelet yield, recovery and concentration were compared before and after PI (n = 20). Platelet quality was assessed by annexin V binding, P-selectin expression and PAC1 binding. Results: The TER pooling set had the highest platelet yield (539 044 x 10(11)) compared with MAC (453 +/- 077) and FRE (456 +/- 051) prior to PI. This was the result of a significantly higher platelet concentration in the TER storage bag (141 +/- 012 x 10(6)/L) compared with MAC (118 +/- 019) and FRE (128 +/- 015). However, the TER platelet content decreased by 156% after PI, yielding 455 +/- 047 x 10(11) platelets compared with smaller reductions at 95% for MAC (410 +/- 069) and 44% for FRE (436 +/- 052). None of the individual PC contained >10(6) leucocytes. The pH in TER PC was lower compared with MAC and FRE caused by a higher lactic acid production rate. Consequently, PAC1 binding after TRAP activation was lowest for TER PC on day 6. P-selectin and annexin V were not different between suppliers. Conclusion: This study demonstrates the added value of evaluating the entire component production process when introducing a new consumable. This study helped to inform a decision on what pooling set is ideally suited for routine implementation taking into account PI.
Keywords
filter, pathogen inactivation, platelets, PATHOGEN INACTIVATION, STORAGE LESION, TRANSFUSION, ACTIVATION

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MLA
Feys, Hendrik, R Devloo, B Sabot, et al. “Comparison of Three Commercially Available Buffy Coat Pooling Sets for the Preparation of Platelet Concentrates.” VOX SANGUINIS 113.6 (2018): 555–561. Print.
APA
Feys, Hendrik, Devloo, R., Sabot, B., Coene, J., & Compernolle, V. (2018). Comparison of three commercially available buffy coat pooling sets for the preparation of platelet concentrates. VOX SANGUINIS, 113(6), 555–561.
Chicago author-date
Feys, Hendrik, R Devloo, B Sabot, J Coene, and Veerle Compernolle. 2018. “Comparison of Three Commercially Available Buffy Coat Pooling Sets for the Preparation of Platelet Concentrates.” Vox Sanguinis 113 (6): 555–561.
Chicago author-date (all authors)
Feys, Hendrik, R Devloo, B Sabot, J Coene, and Veerle Compernolle. 2018. “Comparison of Three Commercially Available Buffy Coat Pooling Sets for the Preparation of Platelet Concentrates.” Vox Sanguinis 113 (6): 555–561.
Vancouver
1.
Feys H, Devloo R, Sabot B, Coene J, Compernolle V. Comparison of three commercially available buffy coat pooling sets for the preparation of platelet concentrates. VOX SANGUINIS. 2018;113(6):555–61.
IEEE
[1]
H. Feys, R. Devloo, B. Sabot, J. Coene, and V. Compernolle, “Comparison of three commercially available buffy coat pooling sets for the preparation of platelet concentrates,” VOX SANGUINIS, vol. 113, no. 6, pp. 555–561, 2018.
@article{8564795,
  abstract     = {Background: A disposable set for platelet concentrate (PC) preparation by the buffy coat method allows pooling of buffy coats, centrifugation and cell separation with in-line leucocyte filtration. This study compares three commercially available pooling sets in combination with INTERCEPT pathogen inactivation (PI). 
Materials and methods: Sets for pooling of buffy coats were from Fresenius Kabi (FRE), Macopharma (MAC) and Terumo BCT (TER). Platelet yield, recovery and concentration were compared before and after PI (n = 20). Platelet quality was assessed by annexin V binding, P-selectin expression and PAC1 binding. 
Results: The TER pooling set had the highest platelet yield (539 044 x 10(11)) compared with MAC (453 +/- 077) and FRE (456 +/- 051) prior to PI. This was the result of a significantly higher platelet concentration in the TER storage bag (141 +/- 012 x 10(6)/L) compared with MAC (118 +/- 019) and FRE (128 +/- 015). However, the TER platelet content decreased by 156% after PI, yielding 455 +/- 047 x 10(11) platelets compared with smaller reductions at 95% for MAC (410 +/- 069) and 44% for FRE (436 +/- 052). None of the individual PC contained >10(6) leucocytes. The pH in TER PC was lower compared with MAC and FRE caused by a higher lactic acid production rate. Consequently, PAC1 binding after TRAP activation was lowest for TER PC on day 6. P-selectin and annexin V were not different between suppliers. 
Conclusion: This study demonstrates the added value of evaluating the entire component production process when introducing a new consumable. This study helped to inform a decision on what pooling set is ideally suited for routine implementation taking into account PI.},
  author       = {Feys, Hendrik and Devloo, R and Sabot, B and Coene, J and Compernolle, Veerle},
  issn         = {0042-9007},
  journal      = {VOX SANGUINIS},
  keywords     = {filter,pathogen inactivation,platelets,PATHOGEN INACTIVATION,STORAGE LESION,TRANSFUSION,ACTIVATION},
  language     = {eng},
  number       = {6},
  pages        = {555--561},
  title        = {Comparison of three commercially available buffy coat pooling sets for the preparation of platelet concentrates},
  url          = {http://dx.doi.org/10.1111/vox.12668},
  volume       = {113},
  year         = {2018},
}

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