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A new Wnt1-CRE TomatoRosa embryonic stem cell line : a tool for studying neural crest cell integration capacity

(2017) STEM CELLS AND DEVELOPMENT. 26(23). p.1682-1694
Author
Organization
Abstract
Neural crest (NC) cells are a migratory, multipotent population giving rise to numerous lineages in the embryo. Their plasticity renders attractive their use in tissue engineering-based therapies, but further knowledge on their in vivo behavior is required before clinical transfer may be envisioned. We here describe the isolation and characterization of a new mouse embryonic stem (ES) line derived from Wnt1-CRE-R26 Rosa(TomatoTdv) blastocyst and show that it displays the characteristics of typical ES cells. Further, these cells can be efficiently directed toward an NC stem cell-like phenotype as attested by concomitant expression of NC marker genes and Tomato fluorescence. As native NC progenitors, they are capable of differentiating toward typical derivative phenotypes and interacting with embryonic tissues to participate in the formation of neo-structures. Their specific fluorescence allows purification and tracking in vivo. This cellular tool should facilitate a better understanding of the mechanisms driving NC fate specification and help identify the key interactions developed within a tissue after in vivo implantation. Altogether, this novel model may provide important knowledge to optimize NC stem cell graft conditions, which are required for efficient tissue repair.
Keywords
Wnt1CRE-Tomato ES cell line, neural crest stem cells, neural crest cell specification, epithelio-mesenchymal tissue re-associations, chick embryo model, IN-VITRO, MOUSE, MULTIPOTENT, FATE, DERIVATION, EXPRESSION, EPITHELIUM, MICE, GENERATION, INDUCTION

Citation

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MLA
Acuna-Mendoza, Soledad et al. “A New Wnt1-CRE TomatoRosa Embryonic Stem Cell Line : a Tool for Studying Neural Crest Cell Integration Capacity.” STEM CELLS AND DEVELOPMENT 26.23 (2017): 1682–1694. Print.
APA
Acuna-Mendoza, S., Martin, S., Kuchler-Bopp, S., Ribes, S., Thalgott, J., Chaussain, C., Creuzet, S., et al. (2017). A new Wnt1-CRE TomatoRosa embryonic stem cell line : a tool for studying neural crest cell integration capacity. STEM CELLS AND DEVELOPMENT, 26(23), 1682–1694.
Chicago author-date
Acuna-Mendoza, Soledad, Sabrina Martin, Sabine Kuchler-Bopp, Sandy Ribes, Jérémy Thalgott, Catherine Chaussain, Sophie Creuzet, Hervé Lesot, Franck Lebrin, and Anne Poliard. 2017. “A New Wnt1-CRE TomatoRosa Embryonic Stem Cell Line : a Tool for Studying Neural Crest Cell Integration Capacity.” Stem Cells and Development 26 (23): 1682–1694.
Chicago author-date (all authors)
Acuna-Mendoza, Soledad, Sabrina Martin, Sabine Kuchler-Bopp, Sandy Ribes, Jérémy Thalgott, Catherine Chaussain, Sophie Creuzet, Hervé Lesot, Franck Lebrin, and Anne Poliard. 2017. “A New Wnt1-CRE TomatoRosa Embryonic Stem Cell Line : a Tool for Studying Neural Crest Cell Integration Capacity.” Stem Cells and Development 26 (23): 1682–1694.
Vancouver
1.
Acuna-Mendoza S, Martin S, Kuchler-Bopp S, Ribes S, Thalgott J, Chaussain C, et al. A new Wnt1-CRE TomatoRosa embryonic stem cell line : a tool for studying neural crest cell integration capacity. STEM CELLS AND DEVELOPMENT. 2017;26(23):1682–94.
IEEE
[1]
S. Acuna-Mendoza et al., “A new Wnt1-CRE TomatoRosa embryonic stem cell line : a tool for studying neural crest cell integration capacity,” STEM CELLS AND DEVELOPMENT, vol. 26, no. 23, pp. 1682–1694, 2017.
@article{8564745,
  abstract     = {Neural crest (NC) cells are a migratory, multipotent population giving rise to numerous lineages in the embryo. Their plasticity renders attractive their use in tissue engineering-based therapies, but further knowledge on their in vivo behavior is required before clinical transfer may be envisioned. We here describe the isolation and characterization of a new mouse embryonic stem (ES) line derived from Wnt1-CRE-R26 Rosa(TomatoTdv) blastocyst and show that it displays the characteristics of typical ES cells. Further, these cells can be efficiently directed toward an NC stem cell-like phenotype as attested by concomitant expression of NC marker genes and Tomato fluorescence. As native NC progenitors, they are capable of differentiating toward typical derivative phenotypes and interacting with embryonic tissues to participate in the formation of neo-structures. Their specific fluorescence allows purification and tracking in vivo. This cellular tool should facilitate a better understanding of the mechanisms driving NC fate specification and help identify the key interactions developed within a tissue after in vivo implantation. Altogether, this novel model may provide important knowledge to optimize NC stem cell graft conditions, which are required for efficient tissue repair.},
  author       = {Acuna-Mendoza, Soledad and Martin, Sabrina and Kuchler-Bopp, Sabine and Ribes, Sandy and Thalgott, Jérémy and Chaussain, Catherine and Creuzet, Sophie and Lesot, Hervé and Lebrin, Franck and Poliard, Anne},
  issn         = {1547-3287},
  journal      = {STEM CELLS AND DEVELOPMENT},
  keywords     = {Wnt1CRE-Tomato ES cell line,neural crest stem cells,neural crest cell specification,epithelio-mesenchymal tissue re-associations,chick embryo model,IN-VITRO,MOUSE,MULTIPOTENT,FATE,DERIVATION,EXPRESSION,EPITHELIUM,MICE,GENERATION,INDUCTION},
  language     = {eng},
  number       = {23},
  pages        = {1682--1694},
  title        = {A new Wnt1-CRE TomatoRosa embryonic stem cell line : a tool for studying neural crest cell integration capacity},
  url          = {http://dx.doi.org/10.1089/scd.2017.0115},
  volume       = {26},
  year         = {2017},
}

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