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The plant hormone ethylene restricts Arabidopsis growth via the epidermis

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Abstract
The gaseous hormone ethylene plays a key role in plant growth and development, and it is a major regulator of stress responses. It inhibits vegetative growth by restricting cell elongation, mainly through cross-talk with auxins. However, it remains unknown whether ethylene controls growth throughout all plant tissues or whether its signaling is confined to specific cell types. We employed a targeted expression approach to map the tissue site(s) of ethylene growth regulation. The ubiquitin E3 ligase complex containing Skp1, Cullin1, and the F-box protein EBF1 or EBF2 (SCFEBF1/2) target the degradation of EIN3, the master transcription factor in ethylene signaling. We coupled EBF1 and EBF2 to a number of cell type-specific promoters. Using phenotypic assays for ethylene response and mutant complementation, we revealed that the epidermis is the main site of ethylene action controlling plant growth in both roots and shoots. Suppression of ethylene signaling in the epidermis of the constitutive ethylene signaling mutant ctr1-1 was sufficient to rescue the mutant phenotype, pointing to the epidermis as a key cell type required for ethylene-mediated growth inhibition.
Keywords
ethylene, auxin, EIN3 binding F-box factor EBF, root/shoot, Arabidopsis, APEX TRANSITION ZONE, IMAGE-ANALYSIS TOOL, F-BOX PROTEINS, ROOT-GROWTH, AUXIN BIOSYNTHESIS, TRANSCRIPTION FACTOR, CELL ELONGATION, TRANSPORT, PATHWAY, EXPRESSION

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Chicago
Vaseva, Irina, Enas Qudeimat, Thomas Potuschak, Yun-Long Du, Pascal Genschik, Filip Vandenbussche, and Dominique Van Der Straeten. 2018. “The Plant Hormone Ethylene Restricts Arabidopsis Growth via the Epidermis.” Proceedings of the National Academy of Sciences of the United States of America 115 (17): E4130–E4139.
APA
Vaseva, I., Qudeimat, E., Potuschak, T., Du, Y.-L., Genschik, P., Vandenbussche, F., & Van Der Straeten, D. (2018). The plant hormone ethylene restricts Arabidopsis growth via the epidermis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 115(17), E4130–E4139.
Vancouver
1.
Vaseva I, Qudeimat E, Potuschak T, Du Y-L, Genschik P, Vandenbussche F, et al. The plant hormone ethylene restricts Arabidopsis growth via the epidermis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2018;115(17):E4130–E4139.
MLA
Vaseva, Irina, Enas Qudeimat, Thomas Potuschak, et al. “The Plant Hormone Ethylene Restricts Arabidopsis Growth via the Epidermis.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.17 (2018): E4130–E4139. Print.
@article{8564138,
  abstract     = {The gaseous hormone ethylene plays a key role in plant growth and development, and it is a major regulator of stress responses. It inhibits vegetative growth by restricting cell elongation, mainly through cross-talk with auxins. However, it remains unknown whether ethylene controls growth throughout all plant tissues or whether its signaling is confined to specific cell types. We employed a targeted expression approach to map the tissue site(s) of ethylene growth regulation. The ubiquitin E3 ligase complex containing Skp1, Cullin1, and the F-box protein EBF1 or EBF2 (SCFEBF1/2) target the degradation of EIN3, the master transcription factor in ethylene signaling. We coupled EBF1 and EBF2 to a number of cell type-specific promoters. Using phenotypic assays for ethylene response and mutant complementation, we revealed that the epidermis is the main site of ethylene action controlling plant growth in both roots and shoots. Suppression of ethylene signaling in the epidermis of the constitutive ethylene signaling mutant ctr1-1 was sufficient to rescue the mutant phenotype, pointing to the epidermis as a key cell type required for ethylene-mediated growth inhibition.},
  author       = {Vaseva, Irina and Qudeimat, Enas and Potuschak, Thomas and Du, Yun-Long and Genschik, Pascal and Vandenbussche, Filip and Van Der Straeten, Dominique},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {ethylene,auxin,EIN3 binding F-box factor EBF,root/shoot,Arabidopsis,APEX TRANSITION ZONE,IMAGE-ANALYSIS TOOL,F-BOX PROTEINS,ROOT-GROWTH,AUXIN BIOSYNTHESIS,TRANSCRIPTION FACTOR,CELL ELONGATION,TRANSPORT,PATHWAY,EXPRESSION},
  language     = {eng},
  number       = {17},
  pages        = {E4130--E4139},
  title        = {The plant hormone ethylene restricts Arabidopsis growth via the epidermis},
  url          = {http://dx.doi.org/10.1073/pnas.1717649115},
  volume       = {115},
  year         = {2018},
}

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