Advanced search
1 file | 578.25 KB

Blastocyst production after intracytoplasmic sperm injection with semen from a stallion with testicular degeneration

Kim Roels (UGent) , Katrien Smits (UGent) , Cyrillus Ververs (UGent) , Jan Govaere (UGent) , Katharina D'Herde (UGent) and Ann Van Soom (UGent)
Author
Organization
Abstract
In horse breeding, intracytoplasmic sperm injection (ICSI) has gained interest to obtain offspring from subfertile individuals. This paper presents a case report of a stallion with severe testicular degeneration. Semen analysis showed very low motility and 83.5% of detached heads. Histology of a testicular biopsy showed severely decreased spermatogenesis, while transmission electron microscopy of the sperm cells revealed no significant abnormalities. A total of 39 oocytes were fertilized by ICSI with frozen-thawed spermatozoa of this stallion: 25 oocytes with intact spermatozoa and 24 with detached heads. When using intact sperm cells, 8 out of the 25 oocytes cleaved, and 1 developed to the blastocyst stage 9 days after ICSI. None of the oocytes injected with a detached sperm head cleaved. Studies on the paternal influence on ICSI outcome are limited in the horse and further research is needed to define which stallion factors may influence ICSI results. Here, we report the possibility to produce a blastocyst by ICSI of a stallion suffering from testicular degeneration with a poor spermiogram, as long as an intact sperm cell containing a centriole is selected.
Keywords
FROZEN-THAWED SPERMATOZOA, IN-VITRO, DECAPITATED SPERMATOZOA, FERTILIZATION, PREGNANCY, OOCYTES, MORPHOLOGY, ICSI, QUALITY

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 578.25 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Roels, Kim, Katrien Smits, Cyrillus Ververs, Jan Govaere, Katharina D’Herde, and Ann Van Soom. 2018. “Blastocyst Production After Intracytoplasmic Sperm Injection with Semen from a Stallion with Testicular Degeneration.” Reproduction in Domestic Animals 53 (3): 814–817.
APA
Roels, Kim, Smits, K., Ververs, C., Govaere, J., D’Herde, K., & Van Soom, A. (2018). Blastocyst production after intracytoplasmic sperm injection with semen from a stallion with testicular degeneration. REPRODUCTION IN DOMESTIC ANIMALS, 53(3), 814–817.
Vancouver
1.
Roels K, Smits K, Ververs C, Govaere J, D’Herde K, Van Soom A. Blastocyst production after intracytoplasmic sperm injection with semen from a stallion with testicular degeneration. REPRODUCTION IN DOMESTIC ANIMALS. 2018;53(3):814–7.
MLA
Roels, Kim, Katrien Smits, Cyrillus Ververs, et al. “Blastocyst Production After Intracytoplasmic Sperm Injection with Semen from a Stallion with Testicular Degeneration.” REPRODUCTION IN DOMESTIC ANIMALS 53.3 (2018): 814–817. Print.
@article{8564013,
  abstract     = {In horse breeding, intracytoplasmic sperm injection (ICSI) has gained interest to obtain offspring from subfertile individuals. This paper presents a case report of a stallion with severe testicular degeneration. Semen analysis showed very low motility and 83.5\% of detached heads. Histology of a testicular biopsy showed severely decreased spermatogenesis, while transmission electron microscopy of the sperm cells revealed no significant abnormalities. A total of 39 oocytes were fertilized by ICSI with frozen-thawed spermatozoa of this stallion: 25 oocytes with intact spermatozoa and 24 with detached heads. When using intact sperm cells, 8 out of the 25 oocytes cleaved, and 1 developed to the blastocyst stage 9 days after ICSI. None of the oocytes injected with a detached sperm head cleaved. Studies on the paternal influence on ICSI outcome are limited in the horse and further research is needed to define which stallion factors may influence ICSI results. Here, we report the possibility to produce a blastocyst by ICSI of a stallion suffering from testicular degeneration with a poor spermiogram, as long as an intact sperm cell containing a centriole is selected.},
  author       = {Roels, Kim and Smits, Katrien and Ververs, Cyrillus and Govaere, Jan and D'Herde, Katharina and Van Soom, Ann},
  issn         = {0936-6768},
  journal      = {REPRODUCTION IN DOMESTIC ANIMALS},
  language     = {eng},
  number       = {3},
  pages        = {814--817},
  title        = {Blastocyst production after intracytoplasmic sperm injection with semen from a stallion with testicular degeneration},
  url          = {http://dx.doi.org/10.1111/rda.13153},
  volume       = {53},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: