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Frequency of occurrence of HIV-1 dual infection in a Belgian MSM population

Laura Hebberecht (UGent) , Leen Vancoillie (UGent) , Marlies Schauvliege (UGent) , Delfien Staelens (UGent) , Kenny Dauwe (UGent) , Virginie Mortier (UGent) and Chris Verhofstede (UGent)
(2018) PLOS ONE. 13(4).
Author
Organization
Abstract
Introduction: HIV-1 dual infection is a condition that results from infection with at least two HIV-1 variants from different sources. The scarceness of information on this condition is partly due to the fact that its detection is technically challenging. Using next-generation sequencing we defined the extent of HIV-1 dual infection in a cohort of men who have sex with men (MSM). Material & methods: Eighty-six MSM, diagnosed with HIV-1 subtype B infection between 2008 and 2013 were selected for next-generation sequencing of the HIV-1 envelope V3. Sequencing was performed on 2 plasma samples collected with an interval of > 6 months before the initiation of antiretroviral therapy. Maximum likelihood phylogenetic trees were inspected for dual infection, defined as the presence of two or more monophyletic clusters with >= 90% bootstrap support and a mean between-cluster genetic distance of >= 10%. To confirm dual infection, deep V3 sequencing of intermediate samples was performed as well as clonal sequencing of the HIV-1 protease-reverse transcriptase gene. Results: Five of the 74 patients (6.8%) for whom deep sequencing was successful, showed clear evidence of dual infection. In 4 of them, the second strain was absent in the first sample but occurred in subsequent samples. This was highly suggestive for superinfection. In 3 patients both virus variants were of subtype B, in 2 patients at least one of the variants was a subtype B/non-B recombinant virus. Conclusions: Dual infection was confirmed in 6.8% of MSM diagnosed with HIV-1 in Belgium. This prevalence is probably an underestimation, because stringent criteria were used to classify viral variants as originating from a new infection event.
Keywords
IMMUNODEFICIENCY-VIRUS TYPE-1, DISEASE PROGRESSION, SEX WORKERS, SUPERINFECTION, SUBTYPE, MEN, RECOMBINATION, SEROCONVERTERS, DIVERSITY, REGION

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Citation

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MLA
Hebberecht, Laura et al. “Frequency of Occurrence of HIV-1 Dual Infection in a Belgian MSM Population.” PLOS ONE 13.4 (2018): n. pag. Print.
APA
Hebberecht, L., Vancoillie, L., Schauvliege, M., Staelens, D., Dauwe, K., Mortier, V., & Verhofstede, C. (2018). Frequency of occurrence of HIV-1 dual infection in a Belgian MSM population. PLOS ONE, 13(4).
Chicago author-date
Hebberecht, Laura, Leen Vancoillie, Marlies Schauvliege, Delfien Staelens, Kenny Dauwe, Virginie Mortier, and Chris Verhofstede. 2018. “Frequency of Occurrence of HIV-1 Dual Infection in a Belgian MSM Population.” Plos One 13 (4).
Chicago author-date (all authors)
Hebberecht, Laura, Leen Vancoillie, Marlies Schauvliege, Delfien Staelens, Kenny Dauwe, Virginie Mortier, and Chris Verhofstede. 2018. “Frequency of Occurrence of HIV-1 Dual Infection in a Belgian MSM Population.” Plos One 13 (4).
Vancouver
1.
Hebberecht L, Vancoillie L, Schauvliege M, Staelens D, Dauwe K, Mortier V, et al. Frequency of occurrence of HIV-1 dual infection in a Belgian MSM population. PLOS ONE. 2018;13(4).
IEEE
[1]
L. Hebberecht et al., “Frequency of occurrence of HIV-1 dual infection in a Belgian MSM population,” PLOS ONE, vol. 13, no. 4, 2018.
@article{8563861,
  abstract     = {Introduction: HIV-1 dual infection is a condition that results from infection with at least two HIV-1 variants from different sources. The scarceness of information on this condition is partly due to the fact that its detection is technically challenging. Using next-generation sequencing we defined the extent of HIV-1 dual infection in a cohort of men who have sex with men (MSM). 
Material & methods: Eighty-six MSM, diagnosed with HIV-1 subtype B infection between 2008 and 2013 were selected for next-generation sequencing of the HIV-1 envelope V3. Sequencing was performed on 2 plasma samples collected with an interval of > 6 months before the initiation of antiretroviral therapy. Maximum likelihood phylogenetic trees were inspected for dual infection, defined as the presence of two or more monophyletic clusters with >= 90% bootstrap support and a mean between-cluster genetic distance of >= 10%. To confirm dual infection, deep V3 sequencing of intermediate samples was performed as well as clonal sequencing of the HIV-1 protease-reverse transcriptase gene. 
Results: Five of the 74 patients (6.8%) for whom deep sequencing was successful, showed clear evidence of dual infection. In 4 of them, the second strain was absent in the first sample but occurred in subsequent samples. This was highly suggestive for superinfection. In 3 patients both virus variants were of subtype B, in 2 patients at least one of the variants was a subtype B/non-B recombinant virus. 
Conclusions: Dual infection was confirmed in 6.8% of MSM diagnosed with HIV-1 in Belgium. This prevalence is probably an underestimation, because stringent criteria were used to classify viral variants as originating from a new infection event.},
  articleno    = {e0195679},
  author       = {Hebberecht, Laura and Vancoillie, Leen and Schauvliege, Marlies and Staelens, Delfien and Dauwe, Kenny and Mortier, Virginie and Verhofstede, Chris},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {IMMUNODEFICIENCY-VIRUS TYPE-1,DISEASE PROGRESSION,SEX WORKERS,SUPERINFECTION,SUBTYPE,MEN,RECOMBINATION,SEROCONVERTERS,DIVERSITY,REGION},
  language     = {eng},
  number       = {4},
  pages        = {15},
  title        = {Frequency of occurrence of HIV-1 dual infection in a Belgian MSM population},
  url          = {http://dx.doi.org/10.1371/journal.pone.0195679},
  volume       = {13},
  year         = {2018},
}

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