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Effect of pH and level of concentrate in the diet on the production of biohydrogenation intermediates in a dual-flow continuous culture

MC Fuentes, S Calsamiglia, PW Cardozo and Bruno Vlaeminck UGent (2009) JOURNAL OF DAIRY SCIENCE. 92(9). p.4456-4466
abstract
Milk fat depression in cows fed high-grain diets has been related to an increase in the concentration of trans-10 C-18:1 and trans-10, cis-12 conjugated linoleic acid (CLA) in milk. These fatty acids (FA) are produced as a result of the alteration in rumen biohydrogenation of dietary unsaturated FA. Because a reduction in ruminal pH is usually observed when high-concentrate diets are fed, the main cause that determines the alteration in the biohydrogenation pathways is not clear. The effect of pH (6.4 vs. 5.6) and dietary forage to concentrate ratios (F:C; 70:30 F:C vs. 30:70 F:C) on rumen microbial fermentation, effluent FA profile, and DNA concentration of bacteria involved in lipolysis and biohydrogenation processes were investigated in a continuous culture trial. The dual-flow continuous culture consisted of 2 periods of 8 d (5 d for adaptation and 3 d for sampling), with a 2 x 2 factorial arrangement of treatments. Samples from solid and liquid mixed effluents were taken for determination of total N, ammonia-N, and volatile fatty acid concentrations, and the remainder of the sample was lyophilized. Dry samples were analyzed for dry matter, ash, neutral and acid detergent fiber, FA, and purine contents. The pH 5.6 reduced organic matter and fiber digestibility, ammonia-N concentration and flow, and crude protein degradation, and increased nonammonia and dietary N flows. The pH 5.6 decreased the flow of C-18:0, trans-11 C-18:1 and cis-9, trans-11 CLA, and increased the flow of trans-10 C-18:1, C18:2n-6, C18:3n-3, trans-11, cis-15 C-18:2 and trans-10, cis-12 CLA in the 1 h after feeding effluent. The pH 5.6 reduced Anaerovibrio lipolytica (32.7 vs. 72.1 pg/10 ng of total DNA) and Butyrivibrio fibrisolvens vaccenic acid subgroup (588 vs. 1,394 pg/10 ng of total DNA) DNA concentrations. The high-concentrate diet increased organic matter and fiber digestibility, nonammonia and bacterial N flows, and reduced ammonia-N concentration and flow. The high-concentrate diet reduced trans-11 C-18:1 and trans-10 C-18:1, and increased C18:2n-6, C18:3n-3 and trans-10, cis-12 CLA proportions in the 1 h after feeding effluent. The increase observed in trans-10, cis-12 CLA proportion in the 1 h after feeding effluent due to the high-concentrate diet was smaller that that observed at pH 5.6. Results indicate that the pH is the main cause of the accumulation of trans-10 C-18:1 and trans-10, cis-12 CLA in the effluent, but the trans-10, cis-12 CLA proportion can be also affected by high levels of concentrate in the diet.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
LACTATING DAIRY-COWS, LINSEED OIL, NUTRITIONAL REGULATION, FATTY-ACIDS, DUODENAL FLOW, RUMEN MICROBIAL FERMENTATION, REAL-TIME PCR, biohydrogenation, concentrate, pH, CONJUGATED LINOLEIC-ACID, polymerase chain reaction, IN-VITRO, BACTERIA
journal title
JOURNAL OF DAIRY SCIENCE
J. Dairy Sci.
volume
92
issue
9
pages
4456 - 4466
Web of Science type
Article
Web of Science id
000269156600037
JCR category
AGRICULTURE, DAIRY & ANIMAL SCIENCE
JCR impact factor
2.463 (2009)
JCR rank
2/49 (2009)
JCR quartile
1 (2009)
ISSN
0022-0302
DOI
10.3168/jds.2008-1722
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
856376
handle
http://hdl.handle.net/1854/LU-856376
date created
2010-02-09 09:17:11
date last changed
2010-09-17 15:58:24
@article{856376,
  abstract     = {Milk fat depression in cows fed high-grain diets has been related to an increase in the concentration of trans-10 C-18:1 and trans-10, cis-12 conjugated linoleic acid (CLA) in milk. These fatty acids (FA) are produced as a result of the alteration in rumen biohydrogenation of dietary unsaturated FA. Because a reduction in ruminal pH is usually observed when high-concentrate diets are fed, the main cause that determines the alteration in the biohydrogenation pathways is not clear. The effect of pH (6.4 vs. 5.6) and dietary forage to concentrate ratios (F:C; 70:30 F:C vs. 30:70 F:C) on rumen microbial fermentation, effluent FA profile, and DNA concentration of bacteria involved in lipolysis and biohydrogenation processes were investigated in a continuous culture trial. The dual-flow continuous culture consisted of 2 periods of 8 d (5 d for adaptation and 3 d for sampling), with a 2 x 2 factorial arrangement of treatments. Samples from solid and liquid mixed effluents were taken for determination of total N, ammonia-N, and volatile fatty acid concentrations, and the remainder of the sample was lyophilized. Dry samples were analyzed for dry matter, ash, neutral and acid detergent fiber, FA, and purine contents. The pH 5.6 reduced organic matter and fiber digestibility, ammonia-N concentration and flow, and crude protein degradation, and increased nonammonia and dietary N flows. The pH 5.6 decreased the flow of C-18:0, trans-11 C-18:1 and cis-9, trans-11 CLA, and increased the flow of trans-10 C-18:1, C18:2n-6, C18:3n-3, trans-11, cis-15 C-18:2 and trans-10, cis-12 CLA in the 1 h after feeding effluent. The pH 5.6 reduced Anaerovibrio lipolytica (32.7 vs. 72.1 pg/10 ng of total DNA) and Butyrivibrio fibrisolvens vaccenic acid subgroup (588 vs. 1,394 pg/10 ng of total DNA) DNA concentrations. The high-concentrate diet increased organic matter and fiber digestibility, nonammonia and bacterial N flows, and reduced ammonia-N concentration and flow. The high-concentrate diet reduced trans-11 C-18:1 and trans-10 C-18:1, and increased C18:2n-6, C18:3n-3 and trans-10, cis-12 CLA proportions in the 1 h after feeding effluent. The increase observed in trans-10, cis-12 CLA proportion in the 1 h after feeding effluent due to the high-concentrate diet was smaller that that observed at pH 5.6. Results indicate that the pH is the main cause of the accumulation of trans-10 C-18:1 and trans-10, cis-12 CLA in the effluent, but the trans-10, cis-12 CLA proportion can be also affected by high levels of concentrate in the diet.},
  author       = {Fuentes, MC and Calsamiglia, S and Cardozo, PW and Vlaeminck, Bruno},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keyword      = {LACTATING DAIRY-COWS,LINSEED OIL,NUTRITIONAL REGULATION,FATTY-ACIDS,DUODENAL FLOW,RUMEN MICROBIAL FERMENTATION,REAL-TIME PCR,biohydrogenation,concentrate,pH,CONJUGATED LINOLEIC-ACID,polymerase chain reaction,IN-VITRO,BACTERIA},
  language     = {eng},
  number       = {9},
  pages        = {4456--4466},
  title        = {Effect of pH and level of concentrate in the diet on the production of biohydrogenation intermediates in a dual-flow continuous culture},
  url          = {http://dx.doi.org/10.3168/jds.2008-1722},
  volume       = {92},
  year         = {2009},
}

Chicago
Fuentes, MC, S Calsamiglia, PW Cardozo, and Bruno Vlaeminck. 2009. “Effect of pH and Level of Concentrate in the Diet on the Production of Biohydrogenation Intermediates in a Dual-flow Continuous Culture.” Journal of Dairy Science 92 (9): 4456–4466.
APA
Fuentes, M., Calsamiglia, S., Cardozo, P., & Vlaeminck, B. (2009). Effect of pH and level of concentrate in the diet on the production of biohydrogenation intermediates in a dual-flow continuous culture. JOURNAL OF DAIRY SCIENCE, 92(9), 4456–4466.
Vancouver
1.
Fuentes M, Calsamiglia S, Cardozo P, Vlaeminck B. Effect of pH and level of concentrate in the diet on the production of biohydrogenation intermediates in a dual-flow continuous culture. JOURNAL OF DAIRY SCIENCE. 2009;92(9):4456–66.
MLA
Fuentes, MC, S Calsamiglia, PW Cardozo, et al. “Effect of pH and Level of Concentrate in the Diet on the Production of Biohydrogenation Intermediates in a Dual-flow Continuous Culture.” JOURNAL OF DAIRY SCIENCE 92.9 (2009): 4456–4466. Print.