Advanced search
1 file | 14.71 MB Add to list

Regulation of intercellular TARGET OF MONOPTEROS 7 protein transport in the Arabidopsis root

(2018) DEVELOPMENT. 145(2).
Author
Organization
Abstract
Intercellular communication coordinates hypophysis establishment in the Arabidopsis embryo. Previously, TARGET OF MONOPTEROS 7 (TMO7) was reported to be transported to the hypophysis, the founder cell of the root cap, and RNA suppression experiments implicated its function in embryonic root development. However, the protein properties and mechanisms mediating TMO7 protein transport, and the role the movement plays in development remained unclear. Here, we report that in the post-embryonic root, TMO7 and its close relatives are transported into the root cap through plasmodesmata in a sequence-dependent manner. We also show that nuclear residence is crucial for TMO7 transport, and postulate that modification, potentially phosphorylation, labels TMO7 for transport. Additionally, three novel CRISPR/Cas9-induced tmo7 alleles confirmed a role in hypophysis division, but suggest complex redundancies with close relatives in root formation. Finally, we demonstrate that TMO7 transport is biologically meaningful, as local expression partially restores hypophysis division in a plasmodesmal protein transport mutant. Our study identifies motifs and amino acids that are pivotal for TMO7 protein transport, and establishes the importance of TMO7 in hypophysis and root development.
Keywords
Author Keywords:TMO7, Plasmodesmata, Cell-cell communication, Protein transport, Embryogenesis, RAM KeyWords Plus:TO-CELL MOVEMENT, TRANSCRIPTION FACTOR, MESSENGER-RNA, TRAFFICKING, PLASMODESMATA, GENE, MERISTEM, EMBRYO, HOMEODOMAIN, PLANTS

Downloads

  • Lu et al. (2018) Development 145,dev152892.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 14.71 MB

Citation

Please use this url to cite or link to this publication:

MLA
Lu, Kuan-Ju et al. “Regulation of Intercellular TARGET OF MONOPTEROS 7 Protein Transport in the Arabidopsis Root.” DEVELOPMENT 145.2 (2018): n. pag. Print.
APA
Lu, K.-J., De Rybel, B., van Mourik, H., & Weijers, D. (2018). Regulation of intercellular TARGET OF MONOPTEROS 7 protein transport in the Arabidopsis root. DEVELOPMENT, 145(2).
Chicago author-date
Lu, Kuan-Ju, Bert De Rybel, Hilda van Mourik, and Dolf Weijers. 2018. “Regulation of Intercellular TARGET OF MONOPTEROS 7 Protein Transport in the Arabidopsis Root.” Development 145 (2).
Chicago author-date (all authors)
Lu, Kuan-Ju, Bert De Rybel, Hilda van Mourik, and Dolf Weijers. 2018. “Regulation of Intercellular TARGET OF MONOPTEROS 7 Protein Transport in the Arabidopsis Root.” Development 145 (2).
Vancouver
1.
Lu K-J, De Rybel B, van Mourik H, Weijers D. Regulation of intercellular TARGET OF MONOPTEROS 7 protein transport in the Arabidopsis root. DEVELOPMENT. 2018;145(2).
IEEE
[1]
K.-J. Lu, B. De Rybel, H. van Mourik, and D. Weijers, “Regulation of intercellular TARGET OF MONOPTEROS 7 protein transport in the Arabidopsis root,” DEVELOPMENT, vol. 145, no. 2, 2018.
@article{8563443,
  abstract     = {Intercellular communication coordinates hypophysis establishment in the Arabidopsis embryo. Previously, TARGET OF MONOPTEROS 7 (TMO7) was reported to be transported to the hypophysis, the founder cell of the root cap, and RNA suppression experiments implicated its function in embryonic root development. However, the protein properties and mechanisms mediating TMO7 protein transport, and the role the movement plays in development remained unclear. Here, we report that in the post-embryonic root, TMO7 and its close relatives are transported into the root cap through plasmodesmata in a sequence-dependent manner. We also show that nuclear residence is crucial for TMO7 transport, and postulate that modification, potentially phosphorylation, labels TMO7 for transport. Additionally, three novel CRISPR/Cas9-induced tmo7 alleles confirmed a role in hypophysis division, but suggest complex redundancies with close relatives in root formation. Finally, we demonstrate that TMO7 transport is biologically meaningful, as local expression partially restores hypophysis division in a plasmodesmal protein transport mutant. Our study identifies motifs and amino acids that are pivotal for TMO7 protein transport, and establishes the importance of TMO7 in hypophysis and root development.},
  articleno    = {dev152892},
  author       = {Lu, Kuan-Ju and De Rybel, Bert and van Mourik, Hilda and Weijers, Dolf},
  issn         = {0950-1991},
  journal      = {DEVELOPMENT},
  keywords     = {Author Keywords:TMO7,Plasmodesmata,Cell-cell communication,Protein transport,Embryogenesis,RAM  KeyWords Plus:TO-CELL MOVEMENT,TRANSCRIPTION FACTOR,MESSENGER-RNA,TRAFFICKING,PLASMODESMATA,GENE,MERISTEM,EMBRYO,HOMEODOMAIN,PLANTS},
  language     = {eng},
  number       = {2},
  pages        = {12},
  title        = {Regulation of intercellular TARGET OF MONOPTEROS 7 protein transport in the Arabidopsis root},
  url          = {http://dx.doi.org/10.1242/dev.152892},
  volume       = {145},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: