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Cold-induced male meiotic restitution in Arabidopsis thaliana is not mediated by GA-DELLA signaling

Bing Liu (UGent) , Nico De Storme (UGent) and Danny Geelen (UGent)
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Abstract
Short periods of cold stress induce male meiotic restitution and diploid pollen formation in Arabidopsis thaliana by specifically interfering with male meiotic cytokinesis. Similar alterations in male meiotic cell division and gametophytic ploidy stability occur when gibberellic acid (GA) signaling is perturbed in developing anthers. In this study, we found that exogenous application of GA primarily induces second division restitution (SDR)-type pollen in Arabidopsis, similar to what cold does. Driven by the close similarity in cellular defects, we tested the hypothesis that cold-induced meiotic restitution is mediated by GA-DELLA signaling. Using a combination of chemical, genetic and cytological approaches, however, we found that both exogenously and endogenously altered GA signaling do not affect the cold sensitivity of male meiotic cytokinesis. Moreover, in vivo localization study using a GFP-tagged version of RGA protein revealed that cold does not affect the expression pattern and abundance of DELLA in Arabidopsis anthers at tetrad stage. Expression study found that transcript of RGA appears enhanced in cold-stressed young flower buds. Since our previous work demonstrated that loss of function of DELLA causes irregular male meiotic cytokinesis, we here conclude that cold-induced meiotic restitution is not mediated by DELLA-dependent GA signaling.
Keywords
GA signaling, cold stress, male meiotic restitution, meiotic cytokinesis, diploid pollen, POLLEN DEVELOPMENT, STRESS RESPONSES, PLANT-RESPONSES, LOW-TEMPERATURE, ABIOTIC STRESS, VISUAL ASSAY, GIBBERELLIN, TOLERANCE, EVOLUTION, PROTEINS

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Citation

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Chicago
Liu, Bing, Nico De Storme, and Danny Geelen. 2018. “Cold-induced Male Meiotic Restitution in Arabidopsis Thaliana Is Not Mediated by GA-DELLA Signaling.” Frontiers in Plant Science 9.
APA
Liu, Bing, De Storme, N., & Geelen, D. (2018). Cold-induced male meiotic restitution in Arabidopsis thaliana is not mediated by GA-DELLA signaling. FRONTIERS IN PLANT SCIENCE, 9.
Vancouver
1.
Liu B, De Storme N, Geelen D. Cold-induced male meiotic restitution in Arabidopsis thaliana is not mediated by GA-DELLA signaling. FRONTIERS IN PLANT SCIENCE. 2018;9.
MLA
Liu, Bing, Nico De Storme, and Danny Geelen. “Cold-induced Male Meiotic Restitution in Arabidopsis Thaliana Is Not Mediated by GA-DELLA Signaling.” FRONTIERS IN PLANT SCIENCE 9 (2018): n. pag. Print.
@article{8563327,
  abstract     = {Short periods of cold stress induce male meiotic restitution and diploid pollen formation in Arabidopsis thaliana by specifically interfering with male meiotic cytokinesis. Similar alterations in male meiotic cell division and gametophytic ploidy stability occur when gibberellic acid (GA) signaling is perturbed in developing anthers. In this study, we found that exogenous application of GA primarily induces second division restitution (SDR)-type pollen in Arabidopsis, similar to what cold does. Driven by the close similarity in cellular defects, we tested the hypothesis that cold-induced meiotic restitution is mediated by GA-DELLA signaling. Using a combination of chemical, genetic and cytological approaches, however, we found that both exogenously and endogenously altered GA signaling do not affect the cold sensitivity of male meiotic cytokinesis. Moreover, in vivo localization study using a GFP-tagged version of RGA protein revealed that cold does not affect the expression pattern and abundance of DELLA in Arabidopsis anthers at tetrad stage. Expression study found that transcript of RGA appears enhanced in cold-stressed young flower buds. Since our previous work demonstrated that loss of function of DELLA causes irregular male meiotic cytokinesis, we here conclude that cold-induced meiotic restitution is not mediated by DELLA-dependent GA signaling.},
  articleno    = {91},
  author       = {Liu, Bing and De Storme, Nico and Geelen, Danny},
  issn         = {1664-462X},
  journal      = {FRONTIERS IN PLANT SCIENCE},
  language     = {eng},
  pages        = {11},
  title        = {Cold-induced male meiotic restitution in Arabidopsis thaliana is not mediated by GA-DELLA signaling},
  url          = {http://dx.doi.org/10.3389/fpls.2018.00091},
  volume       = {9},
  year         = {2018},
}

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