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FIGL1 and its novel partner FLIP form a conserved complex that regulates homologous recombination

(2018) PLOS GENETICS. 14(4).
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Abstract
Homologous recombination is central to repair DNA double-strand breaks, either accidently arising in mitotic cells or in a programed manner at meiosis. Crossovers resulting from the repair of meiotic breaks are essential for proper chromosome segregation and increase genetic diversity of the progeny. However, mechanisms regulating crossover formation remain elusive. Here, we identified through genetic and protein-protein interaction screens FIDGETIN-LIKE-1 INTERACTING PROTEIN (FLIP) as a new partner of the previously characterized anti-crossover factor FIDGETIN-LIKE-1 (FIGL1) in Arabidopsis thaliana. We showed that FLIP limits meiotic crossover together with FIGL1. Further, FLIP and FIGL1 form a protein complex conserved from Arabidopsis to human. FIGL1 interacts with the recombinases RAD51 and DMC1, the enzymes that catalyze the DNA strand exchange step of homologous recombination. Arabidopsis flip mutants recapitulate the figl1 phenotype, with enhanced meiotic recombination associated with change in counts of DMC1 and RAD51 foci. Our data thus suggests that FLIP and FIGL1 form a conserved complex that regulates the crucial step of strand invasion in homologous recombination.
Keywords
LIMIT MEIOTIC CROSSOVERS, STRAND BREAK FORMATION, ARABIDOPSIS-THALIANA, HOLLIDAY JUNCTIONS, CROSSING-OVER, INTERMEDIATE METABOLISM, CHROMOSOME, SYNAPSIS, PROTEIN-SEQUENCE, MEIOSIS, FANCM

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Chicago
Fernandes, Joiselle Blanche, Marine Duhamel, Mathilde Seguéla-Arnaud, Nicole Froger, Chloé Girard, Sandrine Choinard, Victor Solier, et al. 2018. “FIGL1 and Its Novel Partner FLIP Form a Conserved Complex That Regulates Homologous Recombination.” Plos Genetics 14 (4).
APA
Fernandes, J. B., Duhamel, M., Seguéla-Arnaud, M., Froger, N., Girard, C., Choinard, S., Solier, V., et al. (2018). FIGL1 and its novel partner FLIP form a conserved complex that regulates homologous recombination. PLOS GENETICS, 14(4).
Vancouver
1.
Fernandes JB, Duhamel M, Seguéla-Arnaud M, Froger N, Girard C, Choinard S, et al. FIGL1 and its novel partner FLIP form a conserved complex that regulates homologous recombination. PLOS GENETICS. 2018;14(4).
MLA
Fernandes, Joiselle Blanche, Marine Duhamel, Mathilde Seguéla-Arnaud, et al. “FIGL1 and Its Novel Partner FLIP Form a Conserved Complex That Regulates Homologous Recombination.” PLOS GENETICS 14.4 (2018): n. pag. Print.
@article{8563191,
  abstract     = {Homologous recombination is central to repair DNA double-strand breaks, either accidently arising in mitotic cells or in a programed manner at meiosis. Crossovers resulting from the repair of meiotic breaks are essential for proper chromosome segregation and increase genetic diversity of the progeny. However, mechanisms regulating crossover formation remain elusive. Here, we identified through genetic and protein-protein interaction screens FIDGETIN-LIKE-1 INTERACTING PROTEIN (FLIP) as a new partner of the previously characterized anti-crossover factor FIDGETIN-LIKE-1 (FIGL1) in Arabidopsis thaliana. We showed that FLIP limits meiotic crossover together with FIGL1. Further, FLIP and FIGL1 form a protein complex conserved from Arabidopsis to human. FIGL1 interacts with the recombinases RAD51 and DMC1, the enzymes that catalyze the DNA strand exchange step of homologous recombination. Arabidopsis flip mutants recapitulate the figl1 phenotype, with enhanced meiotic recombination associated with change in counts of DMC1 and RAD51 foci. Our data thus suggests that FLIP and FIGL1 form a conserved complex that regulates the crucial step of strand invasion in homologous recombination.},
  articleno    = {e1007317},
  author       = {Fernandes, Joiselle Blanche and Duhamel, Marine and Segu{\'e}la-Arnaud, Mathilde and Froger, Nicole and Girard, Chlo{\'e} and Choinard, Sandrine and Solier, Victor and De Winne, Nancy and De Jaeger, Geert and Gevaert, Kris and Andrey, Philippe and Grelon, Mathilde and Guerois, Raphael and Kumar, Rajeev and Mercier, Rapha{\"e}l},
  issn         = {1553-7404},
  journal      = {PLOS GENETICS},
  keyword      = {LIMIT MEIOTIC CROSSOVERS,STRAND BREAK FORMATION,ARABIDOPSIS-THALIANA,HOLLIDAY JUNCTIONS,CROSSING-OVER,INTERMEDIATE METABOLISM,CHROMOSOME,SYNAPSIS,PROTEIN-SEQUENCE,MEIOSIS,FANCM},
  language     = {eng},
  number       = {4},
  pages        = {22},
  title        = {FIGL1 and its novel partner FLIP form a conserved complex that regulates homologous recombination},
  url          = {http://dx.doi.org/10.1371/journal.pgen.1007317},
  volume       = {14},
  year         = {2018},
}

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