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Vessel-specific reintroduction of CINNAMOYL-COA REDUCTASE1 (CCR1) in dwarfed ccr1 mutants restores vessel and xylary fiber integrity and increases biomass

(2018) PLANT PHYSIOLOGY. 176(1). p.611-633
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Lignocellulosic biomass is recalcitrant toward deconstruction into simple sugars due to the presence of lignin. To render lignocellulosic biomass a suitable feedstock for the bio-based economy, plants can be engineered to have decreased amounts of lignin. However, engineered plants with the lowest amounts of lignin exhibit collapsed vessels and yield penalties. Previous efforts were not able to fully overcome this phenotype without settling in sugar yield upon saccharification. Here, we reintroduced CINNAMOYL-COENZYME A REDUCTASE1 (CCR1) expression specifically in the protoxylem and metaxylem vessel cells of Arabidopsis (Arabidopsis thaliana) ccr1 mutants. The resulting ccr1 ProSNBE: CCR1 lines had overcome the vascular collapse and had a total stem biomass yield that was increased up to 59% as compared with the wild type. Raman analysis showed that monolignols synthesized in the vessels also contribute to the lignification of neighboring xylary fibers. The cell wall composition and metabolome of ccr1 ProSNBE: CCR1 still exhibited many similarities to those of ccr1 mutants, regardless of their yield increase. In contrast to a recent report, the yield penalty of ccr1 mutants was not caused by ferulic acid accumulation but was (largely) the consequence of collapsed vessels. Finally, ccr1 ProSNBE: CCR1 plants had a 4-fold increase in total sugar yield when compared with wild-type plants.
Keywords
SECONDARY CELL-WALL, LIGNIN BIOSYNTHESIS PERTURBATIONS, CONFOCAL RAMAN, MICROSCOPY, SHIKIMATE-ESTERASE CSE, NAC MASTER SWITCHES, DOWN-REGULATION, ARABIDOPSIS-THALIANA, SACCHARIFICATION EFFICIENCY, MONOLIGNOL BIOSYNTHESIS, BIOFUEL PRODUCTION

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Citation

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Chicago
De Meester, Barbara, Lisanne de Vries, Merve Özparpucu, Notburga Gierlinger, Sander Corneillie, Andreas Pallidis, Geert Goeminne, et al. 2018. “Vessel-specific Reintroduction of CINNAMOYL-COA REDUCTASE1 (CCR1) in Dwarfed Ccr1 Mutants Restores Vessel and Xylary Fiber Integrity and Increases Biomass.” Plant Physiology 176 (1): 611–633.
APA
De Meester, Barbara, de Vries, L., Özparpucu, M., Gierlinger, N., Corneillie, S., Pallidis, A., Goeminne, G., et al. (2018). Vessel-specific reintroduction of CINNAMOYL-COA REDUCTASE1 (CCR1) in dwarfed ccr1 mutants restores vessel and xylary fiber integrity and increases biomass. PLANT PHYSIOLOGY, 176(1), 611–633.
Vancouver
1.
De Meester B, de Vries L, Özparpucu M, Gierlinger N, Corneillie S, Pallidis A, et al. Vessel-specific reintroduction of CINNAMOYL-COA REDUCTASE1 (CCR1) in dwarfed ccr1 mutants restores vessel and xylary fiber integrity and increases biomass. PLANT PHYSIOLOGY. 2018;176(1):611–33.
MLA
De Meester, Barbara, Lisanne de Vries, Merve Özparpucu, et al. “Vessel-specific Reintroduction of CINNAMOYL-COA REDUCTASE1 (CCR1) in Dwarfed Ccr1 Mutants Restores Vessel and Xylary Fiber Integrity and Increases Biomass.” PLANT PHYSIOLOGY 176.1 (2018): 611–633. Print.
@article{8563186,
  abstract     = {Lignocellulosic biomass is recalcitrant toward deconstruction into simple sugars due to the presence of lignin. To render lignocellulosic biomass a suitable feedstock for the bio-based economy, plants can be engineered to have decreased amounts of lignin. However, engineered plants with the lowest amounts of lignin exhibit collapsed vessels and yield penalties. Previous efforts were not able to fully overcome this phenotype without settling in sugar yield upon saccharification. Here, we reintroduced CINNAMOYL-COENZYME A REDUCTASE1 (CCR1) expression specifically in the protoxylem and metaxylem vessel cells of Arabidopsis (Arabidopsis thaliana) ccr1 mutants. The resulting ccr1 ProSNBE: CCR1 lines had overcome the vascular collapse and had a total stem biomass yield that was increased up to 59\% as compared with the wild type. Raman analysis showed that monolignols synthesized in the vessels also contribute to the lignification of neighboring xylary fibers. The cell wall composition and metabolome of ccr1 ProSNBE: CCR1 still exhibited many similarities to those of ccr1 mutants, regardless of their yield increase. In contrast to a recent report, the yield penalty of ccr1 mutants was not caused by ferulic acid accumulation but was (largely) the consequence of collapsed vessels. Finally, ccr1 ProSNBE: CCR1 plants had a 4-fold increase in total sugar yield when compared with wild-type plants.},
  author       = {De Meester, Barbara and de Vries, Lisanne and {\"O}zparpucu, Merve and Gierlinger, Notburga and Corneillie, Sander and Pallidis, Andreas and Goeminne, Geert and Morreel, Kris and De Bruyne, Michiel and De Rycke, Riet and Vanholme, Ruben and Boerjan, Wout},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {SECONDARY CELL-WALL,LIGNIN BIOSYNTHESIS PERTURBATIONS,CONFOCAL RAMAN,MICROSCOPY,SHIKIMATE-ESTERASE CSE,NAC MASTER SWITCHES,DOWN-REGULATION,ARABIDOPSIS-THALIANA,SACCHARIFICATION EFFICIENCY,MONOLIGNOL BIOSYNTHESIS,BIOFUEL PRODUCTION},
  language     = {eng},
  number       = {1},
  pages        = {611--633},
  title        = {Vessel-specific reintroduction of CINNAMOYL-COA REDUCTASE1 (CCR1) in dwarfed ccr1 mutants restores vessel and xylary fiber integrity and increases biomass},
  url          = {http://dx.doi.org/10.1104/pp.17.01462},
  volume       = {176},
  year         = {2018},
}

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