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Elevated urinary excretion of free pyridinoline in Friesian horses suggests a breed-specific increase in collagen degradation

Veronique Saey (UGent) , Jonathan Tang, Richard Ducatelle (UGent) , Siska Croubels (UGent) , Siegrid De Baere (UGent) , Stijn Schauvliege (UGent) , Gunther van Loon (UGent) and Koen Chiers (UGent)
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Abstract
Background: Friesian horses are known for their high inbreeding rate resulting in several genetic diseases such as hydrocephaly and dwarfism. This last decade, several studies focused on two other presumed hereditary traits in Friesian horses: megaoesophagus and aortic rupture. The pathogenesis of these diseases remains obscure but an important role of collagen has been hypothesized. The purpose of this study was to examine possible breed-related differences in collagen catabolism. Urinary specimens from Friesian (n = 17, median age 10 years old) and Warmblood horses (n = 17, median age 10 years old) were assessed for mature collagen cross-links, i.e. pyridinoline (PYD) (= hydroxylysylpyridinoline/HP) and deoxypyridinoline (DPD) (lysylpyridinoline /LP). Solid-phase extraction was performed, followed by reversed-phase ion-paired liquid chromatography prior to tandem mass spectrometry (MS/MS) detection. Results: Mean urinary concentrations of free PYD, expressed as fPYD/creatinine ratio, were significantly higher in Friesian horses compared to Warmblood horses (28.5 +/- 5.2 versus 22.2 +/- 9.6 nmol/mmol, p = 0.02) while mean fDPD/creatinine ratios were similar in both horse breeds (3.0 +/- 0.7 versus 4.6 +/- 3.7 nmol/mmol, p = 0.09). Conclusions: Since DPD is considered a specific bone degradation marker and PYD is more widely distributed in connective tissues, the significant elevation in the mean PYD/DPD ratio in Friesian versus Warmblood horses (9.6 +/- 1.6 versus 5.7 +/- 1.8, p < 0.0001) suggests a soft tissue origin for the increased fPYD levels. Considering that a previous study found no differences in total collagen content between Friesian and Warmblood horses for tendon and aortic tissue, this indicates a higher rate of collagen degradation. The latter might, at least in part, explain the predisposition of Friesians to connective tissue disorders.
Keywords
Horse, Aortic rupture, Megaoesophagus, Mass spectrometry, Collagen, Cross-links, PYRIDINIUM CROSSLINKS, BONE-RESORPTION, AORTIC RUPTURE, ELASTIN, AGE, INVOLUTION, PREGNANCY, TURNOVER, DISEASES, MARKERS

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Chicago
Saey, Veronique, Jonathan Tang, Richard Ducatelle, Siska Croubels, Siegrid De Baere, Stijn Schauvliege, Gunther van Loon, and Koen Chiers. 2018. “Elevated Urinary Excretion of Free Pyridinoline in Friesian Horses Suggests a Breed-specific Increase in Collagen Degradation.” Bmc Veterinary Research 14.
APA
Saey, V., Tang, J., Ducatelle, R., Croubels, S., De Baere, S., Schauvliege, S., van Loon, G., et al. (2018). Elevated urinary excretion of free pyridinoline in Friesian horses suggests a breed-specific increase in collagen degradation. BMC VETERINARY RESEARCH, 14.
Vancouver
1.
Saey V, Tang J, Ducatelle R, Croubels S, De Baere S, Schauvliege S, et al. Elevated urinary excretion of free pyridinoline in Friesian horses suggests a breed-specific increase in collagen degradation. BMC VETERINARY RESEARCH. 2018;14.
MLA
Saey, Veronique, Jonathan Tang, Richard Ducatelle, et al. “Elevated Urinary Excretion of Free Pyridinoline in Friesian Horses Suggests a Breed-specific Increase in Collagen Degradation.” BMC VETERINARY RESEARCH 14 (2018): n. pag. Print.
@article{8562421,
  abstract     = {Background: Friesian horses are known for their high inbreeding rate resulting in several genetic diseases such as hydrocephaly and dwarfism. This last decade, several studies focused on two other presumed hereditary traits in Friesian horses: megaoesophagus and aortic rupture. The pathogenesis of these diseases remains obscure but an important role of collagen has been hypothesized. The purpose of this study was to examine possible breed-related differences in collagen catabolism. Urinary specimens from Friesian (n = 17, median age 10 years old) and Warmblood horses (n = 17, median age 10 years old) were assessed for mature collagen cross-links, i.e. pyridinoline (PYD) (= hydroxylysylpyridinoline/HP) and deoxypyridinoline (DPD) (lysylpyridinoline /LP). Solid-phase extraction was performed, followed by reversed-phase ion-paired liquid chromatography prior to tandem mass spectrometry (MS/MS) detection. 
Results: Mean urinary concentrations of free PYD, expressed as fPYD/creatinine ratio, were significantly higher in Friesian horses compared to Warmblood horses (28.5 +/- 5.2 versus 22.2 +/- 9.6 nmol/mmol, p = 0.02) while mean fDPD/creatinine ratios were similar in both horse breeds (3.0 +/- 0.7 versus 4.6 +/- 3.7 nmol/mmol, p = 0.09). 
Conclusions: Since DPD is considered a specific bone degradation marker and PYD is more widely distributed in connective tissues, the significant elevation in the mean PYD/DPD ratio in Friesian versus Warmblood horses (9.6 +/- 1.6 versus 5.7 +/- 1.8, p {\textlangle} 0.0001) suggests a soft tissue origin for the increased fPYD levels. Considering that a previous study found no differences in total collagen content between Friesian and Warmblood horses for tendon and aortic tissue, this indicates a higher rate of collagen degradation. The latter might, at least in part, explain the predisposition of Friesians to connective tissue disorders.},
  articleno    = {139},
  author       = {Saey, Veronique and Tang, Jonathan and Ducatelle, Richard and Croubels, Siska and De Baere, Siegrid and Schauvliege, Stijn and van Loon, Gunther and Chiers, Koen},
  issn         = {1746-6148},
  journal      = {BMC VETERINARY RESEARCH},
  keyword      = {Horse,Aortic rupture,Megaoesophagus,Mass spectrometry,Collagen,Cross-links,PYRIDINIUM CROSSLINKS,BONE-RESORPTION,AORTIC RUPTURE,ELASTIN,AGE,INVOLUTION,PREGNANCY,TURNOVER,DISEASES,MARKERS},
  language     = {eng},
  pages        = {7},
  title        = {Elevated urinary excretion of free pyridinoline in Friesian horses suggests a breed-specific increase in collagen degradation},
  url          = {http://dx.doi.org/10.1186/s12917-018-1454-8},
  volume       = {14},
  year         = {2018},
}

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