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Impact of Acanthamoeba Cysts on Stress Resistance of Salmonella enterica Serovar Typhimurium, Yersinia enterocolitica 4/O:3, Listeria monocytogenes 1/2a, and Escherichia coli O:26

Ellen Lambrecht UGent, Julie Baré UGent, Koen Sabbe UGent and Kurt Houf UGent (2017) APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 83(14).
abstract
The formation of robust resting cysts enables Acanthamoeba to resist harsh environmental conditions. This study investigated to what extent these cysts are resistant to physical and chemical stresses as applied in food industry cleaning and disinfection procedures. Moreover, it was assessed whether certain intracystic meat-borne bacterial pathogens are more stress resistant than free-living bacterial monocultures and if intracystic passage and subsequent association with trophozoites induces cross-tolerance toward other stressors. Several physical and chemical stressors (NaCl, H2O2, benzalkonium chloride, 55 degrees C, heating until boiling, ethanol, dishwashing detergent, and sodium hypochlorite) frequently used in domestic and industrial food-related environments were tested against (i) Acanthamoeba castellanii cysts, (ii) single strains of bacterial monocultures, (iii) intracystic bacteria, and (iv) bacteria after intracystic passage (cyst-primed bacteria). Only heating until boiling and hypochlorite treatment were cysticidal. After boiling, no viable trophozoites could be recovered from the cysts, and hypochlorite treatment caused a 1.34-to 4.72-log10 cells/ml reduction in cyst viability. All treatments were effective in reducing or even eliminating the tested bacterial monocultures, whereas bacteria residing inside cysts were more tolerant toward these stressors. All cyst-primed bacteria exhibited an increased tolerance toward subsequent H2O2 (>92% decrease in median log10 CFU/ml reduction) and 70% ethanol (>99% decrease) treatments. Moreover, intracystic passage significantly increased the survival of Yersinia enterocolitica (74% decrease in median log10 reduction), Escherichia coli (58%), and Salmonella enterica (48%) after NaCl treatment and of E. coli (96%), S. enterica (99%), and Listeria monocytogenes (99%) after sodium hypochlorite treatment compared with that of nonprimed bacteria. IMPORTANCE The results from this study demonstrated that both viable and nonviable amoebal cysts can protect internalized bacteria against stressful conditions. Moreover, cyst passage can induce cross-tolerance in bacteria, increasing their survival when exposed to selected stressors. These findings underscore the potential importance of free-living amoebae in food-related environments and their impact on the persistence of meat-borne bacterial pathogens.
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author
organization
year
type
journalArticle (original)
publication status
published
keyword
CONTACT-LENS DISINFECTANTS, FREE-LIVING AMEBAS, FOODBORNE PATHOGENS, HYDROGEN-PEROXIDE, CASTELLANII, EFFICACY, POLYPHAGA, SURVIVAL, BIOCIDES, PROTOZOA
journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
volume
83
issue
14
article number
UNSP e00754-17
publisher
American Society for Microbiology
ISSN
0099-2240
1098-5336
DOI
10.1128/aem.00754-17
UGent publication?
yes
classification
U
copyright statement
I don't know the status of the copyright for this publication
id
8562208
handle
http://hdl.handle.net/1854/LU-8562208
date created
2018-05-18 08:23:59
date last changed
2018-05-18 08:23:59
@article{8562208,
  abstract     = {The formation of robust resting cysts enables Acanthamoeba to resist harsh environmental conditions. This study investigated to what extent these cysts are resistant to physical and chemical stresses as applied in food industry cleaning and disinfection procedures. Moreover, it was assessed whether certain intracystic meat-borne bacterial pathogens are more stress resistant than free-living bacterial monocultures and if intracystic passage and subsequent association with trophozoites induces cross-tolerance toward other stressors. Several physical and chemical stressors (NaCl, H2O2, benzalkonium chloride, 55 degrees C, heating until boiling, ethanol, dishwashing detergent, and sodium hypochlorite) frequently used in domestic and industrial food-related environments were tested against (i) Acanthamoeba castellanii cysts, (ii) single strains of bacterial monocultures, (iii) intracystic bacteria, and (iv) bacteria after intracystic passage (cyst-primed bacteria). Only heating until boiling and hypochlorite treatment were cysticidal. After boiling, no viable trophozoites could be recovered from the cysts, and hypochlorite treatment caused a 1.34-to 4.72-log10 cells/ml reduction in cyst viability. All treatments were effective in reducing or even eliminating the tested bacterial monocultures, whereas bacteria residing inside cysts were more tolerant toward these stressors. All cyst-primed bacteria exhibited an increased tolerance toward subsequent H2O2 ({\textrangle}92\% decrease in median log10 CFU/ml reduction) and 70\% ethanol ({\textrangle}99\% decrease) treatments. Moreover, intracystic passage significantly increased the survival of Yersinia enterocolitica (74\% decrease in median log10 reduction), Escherichia coli (58\%), and Salmonella enterica (48\%) after NaCl treatment and of E. coli (96\%), S. enterica (99\%), and Listeria monocytogenes (99\%) after sodium hypochlorite treatment compared with that of nonprimed bacteria. 

IMPORTANCE The results from this study demonstrated that both viable and nonviable amoebal cysts can protect internalized bacteria against stressful conditions. Moreover, cyst passage can induce cross-tolerance in bacteria, increasing their survival when exposed to selected stressors. These findings underscore the potential importance of free-living amoebae in food-related environments and their impact on the persistence of meat-borne bacterial pathogens.},
  articleno    = {UNSP e00754-17},
  author       = {Lambrecht, Ellen and Bar{\'e}, Julie and Sabbe, Koen and Houf, Kurt},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {CONTACT-LENS DISINFECTANTS,FREE-LIVING AMEBAS,FOODBORNE PATHOGENS,HYDROGEN-PEROXIDE,CASTELLANII,EFFICACY,POLYPHAGA,SURVIVAL,BIOCIDES,PROTOZOA},
  number       = {14},
  publisher    = {American Society for Microbiology},
  title        = {Impact of Acanthamoeba Cysts on Stress Resistance of Salmonella enterica Serovar Typhimurium, Yersinia enterocolitica 4/O:3, Listeria monocytogenes 1/2a, and Escherichia coli O:26},
  url          = {http://dx.doi.org/10.1128/aem.00754-17},
  volume       = {83},
  year         = {2017},
}

Chicago
Lambrecht, Ellen, Julie Baré, Koen Sabbe, and Kurt Houf. 2017. “Impact of Acanthamoeba Cysts on Stress Resistance of Salmonella Enterica Serovar Typhimurium, Yersinia Enterocolitica 4/O:3, Listeria Monocytogenes 1/2a, and Escherichia Coli O:26.” Applied and Environmental Microbiology 83 (14).
APA
Lambrecht, Ellen, Baré, J., Sabbe, K., & Houf, K. (2017). Impact of Acanthamoeba Cysts on Stress Resistance of Salmonella enterica Serovar Typhimurium, Yersinia enterocolitica 4/O:3, Listeria monocytogenes 1/2a, and Escherichia coli O:26. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(14).
Vancouver
1.
Lambrecht E, Baré J, Sabbe K, Houf K. Impact of Acanthamoeba Cysts on Stress Resistance of Salmonella enterica Serovar Typhimurium, Yersinia enterocolitica 4/O:3, Listeria monocytogenes 1/2a, and Escherichia coli O:26. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology; 2017;83(14).
MLA
Lambrecht, Ellen, Julie Baré, Koen Sabbe, et al. “Impact of Acanthamoeba Cysts on Stress Resistance of Salmonella Enterica Serovar Typhimurium, Yersinia Enterocolitica 4/O:3, Listeria Monocytogenes 1/2a, and Escherichia Coli O:26.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.14 (2017): n. pag. Print.