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Expanding the toolbox for cryopreservation of marine and freshwater diatoms

Willem Stock (UGent), Eveline Pinseel (UGent), Sam De Decker (UGent), Josefin Sefbom (UGent), Lander Blommaert (UGent), Olga Chepurnova (UGent), Koen Sabbe (UGent) and Wim Vyverman (UGent)
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Abstract
Diatoms constitute the most diverse group of microalgae and have long been recognised for their large biotechnological potential. In the wake of growing research interest in new model species and development of commercial applications, there is a pressing need for long-term preservation of diatom strains. While cryopreservation using dimethylsulfoxide (DMSO) as a cryoprotective agent is the preferred method for long-term strain preservation, many diatom species cannot be successfully cryopreserved using DMSO. Therefore, in this study, we studied cryopreservation success in six different diatom species, representing the major morphological and ecological diatom groups, using a range of DMSO concentrations and Plant Vitrification Solution 2 (PVS2) as an alternative cryoprotectant to DMSO. In addition, we tested whether suppressing bacterial growth by antibiotics accelerates the post-thaw recovery process. Our results show that the effects of cryoprotectant choice, its concentration and the addition of antibiotics are highly species specific. In addition, we showed that PVS2 and antibiotics are useful agents to optimize cryopreservation of algae that cannot survive the traditional cryopreservation protocol using DMSO. We conclude that a species-specific approach will remain necessary to develop protocols for diatom cryopreservation and to increase their representation in public culture collections.
Keywords
ENCAPSULATION-VITRIFICATION, PHAEODACTYLUM-TRICORNUTUM, EXPERIMENTAL DESICCATION, GENETIC-TRANSFORMATION, BENTHIC DIATOMS, ALGAE, BACILLARIOPHYCEAE, TOLERANCE, TEMPERATURE, CULTIVATION

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Chicago
Stock, Willem, Eveline Pinseel, Sam De Decker, Josefin Sefbom, Lander Blommaert, Olga Chepurnova, Koen Sabbe, and Wim Vyverman. 2018. “Expanding the Toolbox for Cryopreservation of Marine and Freshwater Diatoms.” Scientific Reports 8.
APA
Stock, W., Pinseel, E., De Decker, S., Sefbom, J., Blommaert, L., Chepurnova, O., Sabbe, K., et al. (2018). Expanding the toolbox for cryopreservation of marine and freshwater diatoms. SCIENTIFIC REPORTS, 8.
Vancouver
1.
Stock W, Pinseel E, De Decker S, Sefbom J, Blommaert L, Chepurnova O, et al. Expanding the toolbox for cryopreservation of marine and freshwater diatoms. SCIENTIFIC REPORTS. 2018;8.
MLA
Stock, Willem, Eveline Pinseel, Sam De Decker, et al. “Expanding the Toolbox for Cryopreservation of Marine and Freshwater Diatoms.” SCIENTIFIC REPORTS 8 (2018): n. pag. Print.
@article{8562131,
  abstract     = {Diatoms constitute the most diverse group of microalgae and have long been recognised for their large biotechnological potential. In the wake of growing research interest in new model species and development of commercial applications, there is a pressing need for long-term preservation of diatom strains. While cryopreservation using dimethylsulfoxide (DMSO) as a cryoprotective agent is the preferred method for long-term strain preservation, many diatom species cannot be successfully cryopreserved using DMSO. Therefore, in this study, we studied cryopreservation success in six different diatom species, representing the major morphological and ecological diatom groups, using a range of DMSO concentrations and Plant Vitrification Solution 2 (PVS2) as an alternative cryoprotectant to DMSO. In addition, we tested whether suppressing bacterial growth by antibiotics accelerates the post-thaw recovery process. Our results show that the effects of cryoprotectant choice, its concentration and the addition of antibiotics are highly species specific. In addition, we showed that PVS2 and antibiotics are useful agents to optimize cryopreservation of algae that cannot survive the traditional cryopreservation protocol using DMSO. We conclude that a species-specific approach will remain necessary to develop protocols for diatom cryopreservation and to increase their representation in public culture collections.},
  articleno    = {4279},
  author       = {Stock, Willem and Pinseel, Eveline and De Decker, Sam and Sefbom, Josefin and Blommaert, Lander and Chepurnova, Olga and Sabbe, Koen and Vyverman, Wim},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keyword      = {ENCAPSULATION-VITRIFICATION,PHAEODACTYLUM-TRICORNUTUM,EXPERIMENTAL DESICCATION,GENETIC-TRANSFORMATION,BENTHIC DIATOMS,ALGAE,BACILLARIOPHYCEAE,TOLERANCE,TEMPERATURE,CULTIVATION},
  language     = {eng},
  pages        = {8},
  title        = {Expanding the toolbox for cryopreservation of marine and freshwater diatoms},
  url          = {http://dx.doi.org/10.1038/s41598-018-22460-0},
  volume       = {8},
  year         = {2018},
}

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