Ghent University Academic Bibliography

Advanced

Rapid tetracycline resistance detection in bovine Pasteurella multocida isolates by MALDI Biotyper antibiotic susceptibility test rapid assay (MBT-ASTRA)

Laura Van Driessche UGent, Jade Bokma UGent, Linde Gille UGent, Pieter-Jan Ceyssens, Katrin Sparbier, Freddy Haesebrouck UGent, Piet Deprez UGent, Filip Boyen UGent and Bart Pardon UGent (2018)
abstract
Pasteurella multocida is notorious for its role as an opportunistic pathogen in infectious bronchopneumonia, the economically most important disease facing cattle industry and leading indication for antimicrobial therapy. To rationalize antimicrobial use, avoiding imprudent use of highly and critically important antimicrobials for human medicine, availability of a rapid antimicrobial susceptibility test is crucial. The objective of the present study was to design a MALDI Biotyper antibiotic susceptibility test rapid assay (MBT-ASTRA) procedure for tetracycline resistance detection in P. multocida. This procedure was validated on 100 clinical isolates with MIC-gradient strip test, and a comparison with disk diffusion was made. Sensitivity and specificity of the MBT-ASTRA procedure were 95.7% (95% confidence interval (CI) = 89.8-101.5) and 100% (95% CI = 100-100), respectively, classifying 98% of the isolates correctly after only three hours of incubation. Sensitivity and specificity of disk diffusion were 91.3% (95% CI = 83.2-99.4) and 92.6% (95% CI = 85.6-99.6) respectively, classifying 91% of the isolates correctly. In conclusion, this MBT-ASTRA procedure has all the potential to fulfil the need for a rapid and highly accurate tetracycline susceptibility testing in P. multocida to rationalize antimicrobial use in outbreaks of bronchopneumonia in cattle or other clinical presentations across species.
Please use this url to cite or link to this publication:
author
organization
year
type
misc (other)
publication status
published
subject
keyword
MBT-ASTRA, fast susceptibility testing, Pasteurella multocida, tetracycline
place of publication
Brussels, Belgium
language
English
UGent publication?
yes
classification
U
id
8562008
handle
http://hdl.handle.net/1854/LU-8562008
date created
2018-05-16 11:30:54
date last changed
2018-05-16 11:41:18
@misc{8562008,
  abstract     = {Pasteurella multocida is notorious for its role as an opportunistic pathogen in infectious bronchopneumonia, the economically most important disease facing cattle industry and leading indication for antimicrobial therapy. To rationalize antimicrobial use, avoiding imprudent use of highly and critically important antimicrobials for human medicine, availability of a rapid antimicrobial susceptibility test is crucial. The objective of the present study was to design a MALDI Biotyper antibiotic susceptibility test rapid assay (MBT-ASTRA) procedure for tetracycline resistance detection in P. multocida. This procedure was validated on 100 clinical isolates with MIC-gradient strip test, and a comparison with disk diffusion was made. Sensitivity and specificity of the MBT-ASTRA procedure were 95.7\% (95\% confidence interval (CI) = 89.8-101.5) and 100\% (95\% CI = 100-100), respectively, classifying 98\% of the isolates correctly after only three hours of incubation. Sensitivity and specificity of disk diffusion were 91.3\% (95\% CI = 83.2-99.4) and 92.6\% (95\% CI = 85.6-99.6) respectively, classifying 91\% of the isolates correctly. In conclusion, this MBT-ASTRA procedure has all the potential to fulfil the need for a rapid and highly accurate tetracycline susceptibility testing in P. multocida to rationalize antimicrobial use in outbreaks of bronchopneumonia in cattle or other clinical presentations across species.},
  author       = {Van Driessche, Laura and Bokma, Jade and Gille, Linde and Ceyssens, Pieter-Jan  and Sparbier, Katrin and Haesebrouck, Freddy and Deprez, Piet and Boyen, Filip and Pardon, Bart},
  keyword      = {MBT-ASTRA,fast susceptibility testing,Pasteurella multocida,tetracycline},
  language     = {eng},
  title        = {Rapid tetracycline resistance detection in bovine Pasteurella multocida isolates by MALDI Biotyper antibiotic susceptibility test rapid assay (MBT-ASTRA)},
  year         = {2018},
}

Chicago
Van Driessche, Laura, Jade Bokma, Linde Gille, Pieter-Jan Ceyssens, Katrin Sparbier, Freddy Haesebrouck, Piet Deprez, Filip Boyen, and Bart Pardon. 2018. “Rapid Tetracycline Resistance Detection in Bovine Pasteurella Multocida Isolates by MALDI Biotyper Antibiotic Susceptibility Test Rapid Assay (MBT-ASTRA)”. Brussels, Belgium.
APA
Van Driessche, Laura, Bokma, J., Gille, L., Ceyssens, P.-J., Sparbier, K., Haesebrouck, F., Deprez, P., et al. (2018). Rapid tetracycline resistance detection in bovine Pasteurella multocida isolates by MALDI Biotyper antibiotic susceptibility test rapid assay (MBT-ASTRA). Brussels, Belgium.
Vancouver
1.
Van Driessche L, Bokma J, Gille L, Ceyssens P-J, Sparbier K, Haesebrouck F, et al. Rapid tetracycline resistance detection in bovine Pasteurella multocida isolates by MALDI Biotyper antibiotic susceptibility test rapid assay (MBT-ASTRA). Brussels, Belgium; 2018.
MLA
Van Driessche, Laura, Jade Bokma, Linde Gille, et al. “Rapid Tetracycline Resistance Detection in Bovine Pasteurella Multocida Isolates by MALDI Biotyper Antibiotic Susceptibility Test Rapid Assay (MBT-ASTRA).” 2018 : n. pag. Print.