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A Functional Study of AUXILIN-LIKE1 and 2, Two Putative Clathrin Uncoating Factors in Arabidopsis

Maciek Adamowski, Madhumitha Narasimhan, Urszula Kania, Matous Glanc, Geert De Jaeger UGent and Jiri Friml (2018) PLANT CELL. 30(3). p.700-716
abstract
Clathrin-mediated endocytosis (CME) is a cellular trafficking process in which cargoes and lipids are internalized from the plasma membrane into vesicles coated with clathrin and adaptor proteins. CME is essential for many developmental and physiological processes in plants, but its underlying mechanism is not well characterized compared with that in yeast and animal systems. Here, we searched for new factors involved in CME in Arabidopsis thaliana by performing tandem affinity purification of proteins that interact with clathrin light chain, a principal component of the clathrin coat. Among the confirmed interactors, we found two putative homologs of the clathrin-coat uncoating factor auxilin previously described in non-plant systems. Overexpression of AUXILIN-LIKE1 and AUXILIN-LIKE2 in Arabidopsis caused an arrest of seedling growth and development. This was concomitant with inhibited endocytosis due to blocking of clathrin recruitment after the initial step of adaptor protein binding to the plasma membrane. By contrast, auxilin-like1/2 loss-of-function lines did not present endocytosis-related developmental or cellular phenotypes under normal growth conditions. This work contributes to the ongoing characterization of the endocytotic machinery in plants and provides a robust tool for conditionally and specifically interfering with CME in Arabidopsis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DYNAMIN-LIKE PROTEIN, GOLGI NETWORK/EARLY ENDOSOME, J-DOMAIN PROTEIN, COATED VESICLES, MEDIATED ENDOCYTOSIS, PLASMA-MEMBRANE, ADAPTER COMPLEX, AUXIN-EFFLUX, INTRACELLULAR TRAFFICKING, INHIBITS ENDOCYTOSIS
journal title
PLANT CELL
Plant Cell
volume
30
issue
3
pages
17 pages
publisher
Amer Soc Plant Biologists
place of publication
Rockville
Web of Science type
Article
Web of Science id
000429441400018
ISSN
1040-4651
1532-298X
DOI
10.1105/tpc.17.00785
language
English
UGent publication?
yes
classification
U
id
8561856
handle
http://hdl.handle.net/1854/LU-8561856
date created
2018-05-15 11:37:00
date last changed
2018-05-15 11:37:00
@article{8561856,
  abstract     = {Clathrin-mediated endocytosis (CME) is a cellular trafficking process in which cargoes and lipids are internalized from the plasma membrane into vesicles coated with clathrin and adaptor proteins. CME is essential for many developmental and physiological processes in plants, but its underlying mechanism is not well characterized compared with that in yeast and animal systems. Here, we searched for new factors involved in CME in Arabidopsis thaliana by performing tandem affinity purification of proteins that interact with clathrin light chain, a principal component of the clathrin coat. Among the confirmed interactors, we found two putative homologs of the clathrin-coat uncoating factor auxilin previously described in non-plant systems. Overexpression of AUXILIN-LIKE1 and AUXILIN-LIKE2 in Arabidopsis caused an arrest of seedling growth and development. This was concomitant with inhibited endocytosis due to blocking of clathrin recruitment after the initial step of adaptor protein binding to the plasma membrane. By contrast, auxilin-like1/2 loss-of-function lines did not present endocytosis-related developmental or cellular phenotypes under normal growth conditions. This work contributes to the ongoing characterization of the endocytotic machinery in plants and provides a robust tool for conditionally and specifically interfering with CME in Arabidopsis.},
  author       = {Adamowski, Maciek and Narasimhan, Madhumitha and Kania, Urszula and Glanc, Matous and De Jaeger, Geert and Friml, Jiri},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {DYNAMIN-LIKE PROTEIN,GOLGI NETWORK/EARLY ENDOSOME,J-DOMAIN PROTEIN,COATED VESICLES,MEDIATED ENDOCYTOSIS,PLASMA-MEMBRANE,ADAPTER COMPLEX,AUXIN-EFFLUX,INTRACELLULAR TRAFFICKING,INHIBITS ENDOCYTOSIS},
  language     = {eng},
  number       = {3},
  pages        = {700--716},
  publisher    = {Amer Soc Plant Biologists},
  title        = {A Functional Study of AUXILIN-LIKE1 and 2, Two Putative Clathrin Uncoating Factors in Arabidopsis},
  url          = {http://dx.doi.org/10.1105/tpc.17.00785},
  volume       = {30},
  year         = {2018},
}

Chicago
Adamowski, Maciek, Madhumitha Narasimhan, Urszula Kania, Matous Glanc, Geert De Jaeger, and Jiri Friml. 2018. “A Functional Study of AUXILIN-LIKE1 and 2, Two Putative Clathrin Uncoating Factors in Arabidopsis.” Plant Cell 30 (3): 700–716.
APA
Adamowski, M., Narasimhan, M., Kania, U., Glanc, M., De Jaeger, G., & Friml, J. (2018). A Functional Study of AUXILIN-LIKE1 and 2, Two Putative Clathrin Uncoating Factors in Arabidopsis. PLANT CELL, 30(3), 700–716.
Vancouver
1.
Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. A Functional Study of AUXILIN-LIKE1 and 2, Two Putative Clathrin Uncoating Factors in Arabidopsis. PLANT CELL. Rockville: Amer Soc Plant Biologists; 2018;30(3):700–16.
MLA
Adamowski, Maciek, Madhumitha Narasimhan, Urszula Kania, et al. “A Functional Study of AUXILIN-LIKE1 and 2, Two Putative Clathrin Uncoating Factors in Arabidopsis.” PLANT CELL 30.3 (2018): 700–716. Print.