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Quantitative tandem affinity purification, an effective tool to investigate protein complex composition in plant hormone signaling : strigolactones in the spotlight

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Abstract
Phytohormones tightly regulate plant growth by integrating changing environmental and developmental cues. Although the key players have been identified in many plant hormonal pathways, the molecular mechanisms and mode of action of perception and signaling remain incompletely resolved. Characterization of protein partners of known signaling components provides insight into the formed protein complexes, but, unless quantification is involved, does not deliver much, if any, information about the dynamics of the induced or disrupted protein complexes. Therefore, in proteomics research, the discovery of what actually triggers, regulates or interrupts the composition of protein complexes is gaining importance. Here, tandem affinity purification coupled to mass spectrometry (TAP-MS) is combined with label-free quantification (LFQ) to a highly valuable tool to detect physiologically relevant, dynamic protein-protein interactions in Arabidopsis thaliana cell cultures. To demonstrate its potential, we focus on the signaling pathway of one of the most recently discovered phytohormones, strigolactones.
Keywords
TRANSCRIPTION FACTOR COMPLEXES, ARABIDOPSIS-THALIANA, MASS-SPECTROMETRY, SHOOT DEVELOPMENT, REPRESSOR, IDENTIFICATION, DEGRADATION, PROTEOMICS, GROWTH, QUANTIFICATION, strigolactones, SMXL7, qTAP, plant hormone signaling, protein dynamics

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Chicago
Struk, Sylwia Magdalena, Lukas Braem, Alan Walton, Annick De Keyser, François-Didier Boyer, Geert Persiau, Geert De Jaeger, Kris Gevaert, and Sofie Goormachtig. 2018. “Quantitative Tandem Affinity Purification, an Effective Tool to Investigate Protein Complex Composition in Plant Hormone Signaling : Strigolactones in the Spotlight.” Frontiers in Plant Science 9.
APA
Struk, S. M., Braem, L., Walton, A., De Keyser, A., Boyer, F.-D., Persiau, G., De Jaeger, G., et al. (2018). Quantitative tandem affinity purification, an effective tool to investigate protein complex composition in plant hormone signaling : strigolactones in the spotlight. FRONTIERS IN PLANT SCIENCE, 9.
Vancouver
1.
Struk SM, Braem L, Walton A, De Keyser A, Boyer F-D, Persiau G, et al. Quantitative tandem affinity purification, an effective tool to investigate protein complex composition in plant hormone signaling : strigolactones in the spotlight. FRONTIERS IN PLANT SCIENCE. 2018;9.
MLA
Struk, Sylwia Magdalena, Lukas Braem, Alan Walton, et al. “Quantitative Tandem Affinity Purification, an Effective Tool to Investigate Protein Complex Composition in Plant Hormone Signaling : Strigolactones in the Spotlight.” FRONTIERS IN PLANT SCIENCE 9 (2018): n. pag. Print.
@article{8561832,
  abstract     = {Phytohormones tightly regulate plant growth by integrating changing environmental and developmental cues. Although the key players have been identified in many plant hormonal pathways, the molecular mechanisms and mode of action of perception and signaling remain incompletely resolved. Characterization of protein partners of known signaling components provides insight into the formed protein complexes, but, unless quantification is involved, does not deliver much, if any, information about the dynamics of the induced or disrupted protein complexes. Therefore, in proteomics research, the discovery of what actually triggers, regulates or interrupts the composition of protein complexes is gaining importance. Here, tandem affinity purification coupled to mass spectrometry (TAP-MS) is combined with label-free quantification (LFQ) to a highly valuable tool to detect physiologically relevant, dynamic protein-protein interactions in Arabidopsis thaliana cell cultures. To demonstrate its potential, we focus on the signaling pathway of one of the most recently discovered phytohormones, strigolactones.},
  articleno    = {528},
  author       = {Struk, Sylwia Magdalena and Braem, Lukas and Walton, Alan and De Keyser, Annick and Boyer, Fran\c{c}ois-Didier and Persiau, Geert and De Jaeger, Geert and Gevaert, Kris and Goormachtig, Sofie},
  issn         = {1664-462X},
  journal      = {FRONTIERS IN PLANT SCIENCE},
  keyword      = {TRANSCRIPTION FACTOR COMPLEXES,ARABIDOPSIS-THALIANA,MASS-SPECTROMETRY,SHOOT DEVELOPMENT,REPRESSOR,IDENTIFICATION,DEGRADATION,PROTEOMICS,GROWTH,QUANTIFICATION,strigolactones,SMXL7,qTAP,plant hormone signaling,protein dynamics},
  language     = {eng},
  pages        = {12},
  title        = {Quantitative tandem affinity purification, an effective tool to investigate protein complex composition in plant hormone signaling : strigolactones in the spotlight},
  url          = {http://dx.doi.org/10.3389/fpls.2018.00528},
  volume       = {9},
  year         = {2018},
}

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