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Quantitative Tandem Affinity Purification, an Effective Tool to Investigate Protein Complex Composition in Plant Hormone Signaling: Strigolactones in the Spotlight

Sylwia Magdalena Struk UGent, Lukas Braem UGent, Alan Walton, Annick De Keyser UGent, François-Didier Boyer, Geert Persiau UGent, Geert De Jaeger UGent, Kris Gevaert UGent and Sofie Goormachtig UGent (2018) FRONTIERS IN PLANT SCIENCE. 9.
abstract
Phytohormones tightly regulate plant growth by integrating changing environmental and developmental cues. Although the key players have been identified in many plant hormonal pathways, the molecular mechanisms and mode of action of perception and signaling remain incompletely resolved. Characterization of protein partners of known signaling components provides insight into the formed protein complexes, but, unless quantification is involved, does not deliver much, if any, information about the dynamics of the induced or disrupted protein complexes. Therefore, in proteomics research, the discovery of what actually triggers, regulates or interrupts the composition of protein complexes is gaining importance. Here, tandem affinity purification coupled to mass spectrometry (TAP-MS) is combined with label-free quantification (LFQ) to a highly valuable tool to detect physiologically relevant, dynamic protein-protein interactions in Arabidopsis thaliana cell cultures. To demonstrate its potential, we focus on the signaling pathway of one of the most recently discovered phytohormones, strigolactones.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
TRANSCRIPTION FACTOR COMPLEXES, ARABIDOPSIS-THALIANA, MASS-SPECTROMETRY, SHOOT DEVELOPMENT, REPRESSOR, IDENTIFICATION, DEGRADATION, PROTEOMICS, GROWTH, QUANTIFICATION, strigolactones, SMXL7, qTAP, plant hormone signaling, protein dynamics
journal title
FRONTIERS IN PLANT SCIENCE
Front. Plant Sci.
volume
9
article number
528
pages
12 pages
publisher
Frontiers Media Sa
place of publication
Lausanne
Web of Science type
Article
Web of Science id
000430929000001
ISSN
1664-462X
DOI
10.3389/fpls.2018.00528
language
English
UGent publication?
yes
classification
U
id
8561832
handle
http://hdl.handle.net/1854/LU-8561832
date created
2018-05-15 11:18:24
date last changed
2018-05-15 11:18:24
@article{8561832,
  abstract     = {Phytohormones tightly regulate plant growth by integrating changing environmental and developmental cues. Although the key players have been identified in many plant hormonal pathways, the molecular mechanisms and mode of action of perception and signaling remain incompletely resolved. Characterization of protein partners of known signaling components provides insight into the formed protein complexes, but, unless quantification is involved, does not deliver much, if any, information about the dynamics of the induced or disrupted protein complexes. Therefore, in proteomics research, the discovery of what actually triggers, regulates or interrupts the composition of protein complexes is gaining importance. Here, tandem affinity purification coupled to mass spectrometry (TAP-MS) is combined with label-free quantification (LFQ) to a highly valuable tool to detect physiologically relevant, dynamic protein-protein interactions in Arabidopsis thaliana cell cultures. To demonstrate its potential, we focus on the signaling pathway of one of the most recently discovered phytohormones, strigolactones.},
  articleno    = {528},
  author       = {Struk, Sylwia Magdalena and Braem, Lukas and Walton, Alan and De Keyser, Annick and Boyer, Fran\c{c}ois-Didier and Persiau, Geert and De Jaeger, Geert and Gevaert, Kris and Goormachtig, Sofie},
  issn         = {1664-462X},
  journal      = {FRONTIERS IN PLANT SCIENCE},
  keyword      = {TRANSCRIPTION FACTOR COMPLEXES,ARABIDOPSIS-THALIANA,MASS-SPECTROMETRY,SHOOT DEVELOPMENT,REPRESSOR,IDENTIFICATION,DEGRADATION,PROTEOMICS,GROWTH,QUANTIFICATION,strigolactones,SMXL7,qTAP,plant hormone signaling,protein dynamics},
  language     = {eng},
  pages        = {12},
  publisher    = {Frontiers Media Sa},
  title        = {Quantitative Tandem Affinity Purification, an Effective Tool to Investigate Protein Complex Composition in Plant Hormone Signaling: Strigolactones in the Spotlight},
  url          = {http://dx.doi.org/10.3389/fpls.2018.00528},
  volume       = {9},
  year         = {2018},
}

Chicago
Struk, Sylwia Magdalena, Lukas Braem, Alan Walton, Annick De Keyser, François-Didier Boyer, Geert Persiau, Geert De Jaeger, Kris Gevaert, and Sofie Goormachtig. 2018. “Quantitative Tandem Affinity Purification, an Effective Tool to Investigate Protein Complex Composition in Plant Hormone Signaling: Strigolactones in the Spotlight.” Frontiers in Plant Science 9.
APA
Struk, S. M., Braem, L., Walton, A., De Keyser, A., Boyer, F.-D., Persiau, G., De Jaeger, G., et al. (2018). Quantitative Tandem Affinity Purification, an Effective Tool to Investigate Protein Complex Composition in Plant Hormone Signaling: Strigolactones in the Spotlight. FRONTIERS IN PLANT SCIENCE, 9.
Vancouver
1.
Struk SM, Braem L, Walton A, De Keyser A, Boyer F-D, Persiau G, et al. Quantitative Tandem Affinity Purification, an Effective Tool to Investigate Protein Complex Composition in Plant Hormone Signaling: Strigolactones in the Spotlight. FRONTIERS IN PLANT SCIENCE. Lausanne: Frontiers Media Sa; 2018;9.
MLA
Struk, Sylwia Magdalena, Lukas Braem, Alan Walton, et al. “Quantitative Tandem Affinity Purification, an Effective Tool to Investigate Protein Complex Composition in Plant Hormone Signaling: Strigolactones in the Spotlight.” FRONTIERS IN PLANT SCIENCE 9 (2018): n. pag. Print.