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Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background

Sabitri Ghimire, Margot Van der Jeught UGent, Jitesh Neupane, Matthias S. Roost, Jasper Anckaert UGent, Mina Popovic UGent, Filip Van Nieuwerburgh UGent, Pieter Mestdagh UGent, Jo Vandesompele UGent, Dieter Deforce UGent, et al. (2018) SCIENTIFIC REPORTS. 8.
abstract
Mouse embryonic stem cells (mESCs) exist in a naive, primed and ground state of pluripotency. While comparative analyses of these pluripotency states have been reported, the mESCs utilized originated from various genetic backgrounds and were derived in different laboratories. mESC derivation in conventional LIF + serum culture conditions is strain dependent, with different genetic backgrounds potentially affecting subsequent stem cell characteristics. In the present study, we performed a comprehensive characterization of naive, primed and ground state mESCs originating from the same genetic background within our laboratory, by comparing their transcriptional profiles. We showed unique transcriptional profiles for naive, primed and ground state mESCs. While naive and ground state mESCs have more similar but not identical profiles, primed state mESCs show a very distinct profile. We further demonstrate that the differentiation propensity of mESCs to specific germ layers is highly dependent on their respective state of pluripotency.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
keyword
LINES, ESTABLISHMENT, DERIVATION, INHIBITION, CULTURE
journal title
SCIENTIFIC REPORTS
Sci Rep
volume
8
article number
5884
pages
11 pages
publisher
Nature Publishing Group
place of publication
London
Web of Science type
Article
Web of Science id
000429785900024
ISSN
2045-2322
DOI
10.1038/s41598-018-24051-5
language
English
UGent publication?
yes
classification
U
id
8561817
handle
http://hdl.handle.net/1854/LU-8561817
date created
2018-05-15 09:55:13
date last changed
2018-05-15 09:55:26
@article{8561817,
  abstract     = {Mouse embryonic stem cells (mESCs) exist in a naive, primed and ground state of pluripotency. While comparative analyses of these pluripotency states have been reported, the mESCs utilized originated from various genetic backgrounds and were derived in different laboratories. mESC derivation in conventional LIF + serum culture conditions is strain dependent, with different genetic backgrounds potentially affecting subsequent stem cell characteristics. In the present study, we performed a comprehensive characterization of naive, primed and ground state mESCs originating from the same genetic background within our laboratory, by comparing their transcriptional profiles. We showed unique transcriptional profiles for naive, primed and ground state mESCs. While naive and ground state mESCs have more similar but not identical profiles, primed state mESCs show a very distinct profile. We further demonstrate that the differentiation propensity of mESCs to specific germ layers is highly dependent on their respective state of pluripotency.},
  articleno    = {5884},
  author       = {Ghimire, Sabitri and Van der Jeught, Margot and Neupane, Jitesh and Roost, Matthias S. and Anckaert, Jasper and Popovic, Mina and Van Nieuwerburgh, Filip and Mestdagh, Pieter and Vandesompele, Jo and Deforce, Dieter and Menten, Bj{\"o}rn and Lopes, Susana Chuva de Sousa and De Sutter, Petra and Heindryckx, Bj{\"o}rn},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keyword      = {LINES,ESTABLISHMENT,DERIVATION,INHIBITION,CULTURE},
  language     = {eng},
  pages        = {11},
  publisher    = {Nature Publishing Group},
  title        = {Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background},
  url          = {http://dx.doi.org/10.1038/s41598-018-24051-5},
  volume       = {8},
  year         = {2018},
}

Chicago
Ghimire, Sabitri, Margot Van der Jeught, Jitesh Neupane, Matthias S. Roost, Jasper Anckaert, Mina Popovic, Filip Van Nieuwerburgh, et al. 2018. “Comparative Analysis of Naive, Primed and Ground State Pluripotency in Mouse Embryonic Stem Cells Originating from the Same Genetic Background.” Scientific Reports 8.
APA
Ghimire, S., Van der Jeught, M., Neupane, J., Roost, M. S., Anckaert, J., Popovic, M., Van Nieuwerburgh, F., et al. (2018). Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background. SCIENTIFIC REPORTS, 8.
Vancouver
1.
Ghimire S, Van der Jeught M, Neupane J, Roost MS, Anckaert J, Popovic M, et al. Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background. SCIENTIFIC REPORTS. London: Nature Publishing Group; 2018;8.
MLA
Ghimire, Sabitri, Margot Van der Jeught, Jitesh Neupane, et al. “Comparative Analysis of Naive, Primed and Ground State Pluripotency in Mouse Embryonic Stem Cells Originating from the Same Genetic Background.” SCIENTIFIC REPORTS 8 (2018): n. pag. Print.