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Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background

Sabitri Ghimire (UGent) , Margot Van der Jeught (UGent) , Jitesh Neupane (UGent) , Matthias S Roost, Jasper Anckaert (UGent) , Mina Popovic (UGent) , Filip Van Nieuwerburgh (UGent) , Pieter Mestdagh (UGent) , Jo Vandesompele (UGent) , Dieter Deforce (UGent) , et al.
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Abstract
Mouse embryonic stem cells (mESCs) exist in a naive, primed and ground state of pluripotency. While comparative analyses of these pluripotency states have been reported, the mESCs utilized originated from various genetic backgrounds and were derived in different laboratories. mESC derivation in conventional LIF + serum culture conditions is strain dependent, with different genetic backgrounds potentially affecting subsequent stem cell characteristics. In the present study, we performed a comprehensive characterization of naive, primed and ground state mESCs originating from the same genetic background within our laboratory, by comparing their transcriptional profiles. We showed unique transcriptional profiles for naive, primed and ground state mESCs. While naive and ground state mESCs have more similar but not identical profiles, primed state mESCs show a very distinct profile. We further demonstrate that the differentiation propensity of mESCs to specific germ layers is highly dependent on their respective state of pluripotency.
Keywords
LINES, ESTABLISHMENT, DERIVATION, INHIBITION, CULTURE

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MLA
Ghimire, Sabitri, Margot Van der Jeught, Jitesh Neupane, et al. “Comparative Analysis of Naive, Primed and Ground State Pluripotency in Mouse Embryonic Stem Cells Originating from the Same Genetic Background.” SCIENTIFIC REPORTS 8 (2018): n. pag. Print.
APA
Ghimire, S., Van der Jeught, M., Neupane, J., Roost, M. S., Anckaert, J., Popovic, M., Van Nieuwerburgh, F., et al. (2018). Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background. SCIENTIFIC REPORTS, 8.
Chicago author-date
Ghimire, Sabitri, Margot Van der Jeught, Jitesh Neupane, Matthias S Roost, Jasper Anckaert, Mina Popovic, Filip Van Nieuwerburgh, et al. 2018. “Comparative Analysis of Naive, Primed and Ground State Pluripotency in Mouse Embryonic Stem Cells Originating from the Same Genetic Background.” Scientific Reports 8.
Chicago author-date (all authors)
Ghimire, Sabitri, Margot Van der Jeught, Jitesh Neupane, Matthias S Roost, Jasper Anckaert, Mina Popovic, Filip Van Nieuwerburgh, Pieter Mestdagh, Jo Vandesompele, Dieter Deforce, Björn Menten, Susana Chuva de Sousa Lopes, Petra De Sutter, and Björn Heindryckx. 2018. “Comparative Analysis of Naive, Primed and Ground State Pluripotency in Mouse Embryonic Stem Cells Originating from the Same Genetic Background.” Scientific Reports 8.
Vancouver
1.
Ghimire S, Van der Jeught M, Neupane J, Roost MS, Anckaert J, Popovic M, et al. Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background. SCIENTIFIC REPORTS. 2018;8.
IEEE
[1]
S. Ghimire et al., “Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background,” SCIENTIFIC REPORTS, vol. 8, 2018.
@article{8561817,
  abstract     = {Mouse embryonic stem cells (mESCs) exist in a naive, primed and ground state of pluripotency. While comparative analyses of these pluripotency states have been reported, the mESCs utilized originated from various genetic backgrounds and were derived in different laboratories. mESC derivation in conventional LIF + serum culture conditions is strain dependent, with different genetic backgrounds potentially affecting subsequent stem cell characteristics. In the present study, we performed a comprehensive characterization of naive, primed and ground state mESCs originating from the same genetic background within our laboratory, by comparing their transcriptional profiles. We showed unique transcriptional profiles for naive, primed and ground state mESCs. While naive and ground state mESCs have more similar but not identical profiles, primed state mESCs show a very distinct profile. We further demonstrate that the differentiation propensity of mESCs to specific germ layers is highly dependent on their respective state of pluripotency.},
  articleno    = {5884},
  author       = {Ghimire, Sabitri and Van der Jeught, Margot and Neupane, Jitesh and Roost, Matthias S and Anckaert, Jasper and Popovic, Mina and Van Nieuwerburgh, Filip and Mestdagh, Pieter and Vandesompele, Jo and Deforce, Dieter and Menten, Björn and Lopes, Susana Chuva de Sousa and De Sutter, Petra and Heindryckx, Björn},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {LINES,ESTABLISHMENT,DERIVATION,INHIBITION,CULTURE},
  language     = {eng},
  pages        = {11},
  title        = {Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background},
  url          = {http://dx.doi.org/10.1038/s41598-018-24051-5},
  volume       = {8},
  year         = {2018},
}

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