Ghent University Academic Bibliography

Advanced

Characterization of cefotaxime- and ciprofloxacin-resistant commensal Escherichia coli originating from Belgian farm animals indicates high antibiotic resistance transfer rates

Ellen Lambrecht UGent, Eva Van Meervenne, Nico Boon UGent, Tom Van de Wiele UGent, Pierre Wattiau, Lieve Herman, Marc Heyndrickx UGent and Els Van Coillie (2017) MICROBIAL DRUG RESISTANCE.
abstract
Food-producing animals represent one of the sources of antibiotic resistant commensal bacteria. There is an increasing awareness that these bacteria might have the potential to transfer their resistance genes to other (pathogenic) bacteria. In this study, 50 commensal Escherichia coli strains originating from food-producing animals and resistant to the highest priority, critically important antibiotics cefotaxime and/or ciprofloxacin, were selected for further characterization. For each strain (i) an antibiogram, (ii) the phylogenetic group, (iii) plasmid replicon type, (iv) presence and identification of integrons, and (v) antibiotic resistance transfer ratios were determined. Forty-five of these strains were resistant to 5 or more antibiotics, and 6 strains were resistant to 10 or more antibiotics. Resistance was most common to ampicillin (100%), sulfamethoxazole, ciprofloxacin (82%), trimethoprim, tetracycline (74%), cefotaxime, (70%) and ceftazidime (62%). Phylogenetic groups A (62%) and B1 (26%) were most common, followed by C (8%) and E (4%). In 43 strains, more than 1 replicon type was detected, with FII (88%), FIB (70%), and I1 (48%) being the most encountered types. Forty strains, positive for integrons, all harbored a class I integron and seven of them contained an additional class II integron. No class III integrons were detected. The antibiotic resistance transfer was assessed by liquid mating experiments. The transfer ratio, expressed as the number of transconjugants per recipient, was between 10(-5) and 10(0) for cefotaxime resistance and between 10(-7) and 10(-1) for ciprofloxacin resistance. The results of the current study prove that commensal E. coli in food-production animals can be a source of multiple resistance genes and that these bacteria can easily spread their ciprofloxacin and cefotaxime resistance.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
in press
subject
keyword
FOOD-PRODUCING ANIMALS, ANTIMICROBIAL RESISTANCE, GENE CASSETTES, PHYLOGENETIC GROUPS, 2 INTEGRONS, PLASMIDS, PREVALENCE, ANCIENT, CEPHALOSPORINS, IDENTIFICATION, cefotaxime, ciprofloxacin, transfer ratio, resistance, commensal E, coli
journal title
MICROBIAL DRUG RESISTANCE
Microb. Drug Resist.
pages
11 pages
Web of Science type
Article; Early Access
Web of Science id
000417110000001
ISSN
1076-6294
1931-8448
DOI
10.1089/mdr.2017.0226
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8560339
handle
http://hdl.handle.net/1854/LU-8560339
date created
2018-04-27 12:30:48
date last changed
2018-06-06 13:57:19
@article{8560339,
  abstract     = {Food-producing animals represent one of the sources of antibiotic resistant commensal bacteria. There is an increasing awareness that these bacteria might have the potential to transfer their resistance genes to other (pathogenic) bacteria. In this study, 50 commensal Escherichia coli strains originating from food-producing animals and resistant to the highest priority, critically important antibiotics cefotaxime and/or ciprofloxacin, were selected for further characterization. For each strain (i) an antibiogram, (ii) the phylogenetic group, (iii) plasmid replicon type, (iv) presence and identification of integrons, and (v) antibiotic resistance transfer ratios were determined. Forty-five of these strains were resistant to 5 or more antibiotics, and 6 strains were resistant to 10 or more antibiotics. Resistance was most common to ampicillin (100\%), sulfamethoxazole, ciprofloxacin (82\%), trimethoprim, tetracycline (74\%), cefotaxime, (70\%) and ceftazidime (62\%). Phylogenetic groups A (62\%) and B1 (26\%) were most common, followed by C (8\%) and E (4\%). In 43 strains, more than 1 replicon type was detected, with FII (88\%), FIB (70\%), and I1 (48\%) being the most encountered types. Forty strains, positive for integrons, all harbored a class I integron and seven of them contained an additional class II integron. No class III integrons were detected. The antibiotic resistance transfer was assessed by liquid mating experiments. The transfer ratio, expressed as the number of transconjugants per recipient, was between 10(-5) and 10(0) for cefotaxime resistance and between 10(-7) and 10(-1) for ciprofloxacin resistance. The results of the current study prove that commensal E. coli in food-production animals can be a source of multiple resistance genes and that these bacteria can easily spread their ciprofloxacin and cefotaxime resistance.},
  author       = {Lambrecht, Ellen and Van Meervenne, Eva and Boon, Nico and Van de Wiele, Tom and Wattiau, Pierre and Herman, Lieve and Heyndrickx, Marc and Van Coillie, Els},
  issn         = {1076-6294},
  journal      = {MICROBIAL DRUG RESISTANCE},
  keyword      = {FOOD-PRODUCING ANIMALS,ANTIMICROBIAL RESISTANCE,GENE CASSETTES,PHYLOGENETIC GROUPS,2 INTEGRONS,PLASMIDS,PREVALENCE,ANCIENT,CEPHALOSPORINS,IDENTIFICATION,cefotaxime,ciprofloxacin,transfer ratio,resistance,commensal E,coli},
  language     = {eng},
  pages        = {11},
  title        = {Characterization of cefotaxime- and ciprofloxacin-resistant commensal Escherichia coli originating from Belgian farm animals indicates high antibiotic resistance transfer rates},
  url          = {http://dx.doi.org/10.1089/mdr.2017.0226},
  year         = {2017},
}

Chicago
Lambrecht, Ellen, Eva Van Meervenne, Nico Boon, Tom Van de Wiele, Pierre Wattiau, Lieve Herman, Marc Heyndrickx, and Els Van Coillie. 2017. “Characterization of Cefotaxime- and Ciprofloxacin-resistant Commensal Escherichia Coli Originating from Belgian Farm Animals Indicates High Antibiotic Resistance Transfer Rates.” Microbial Drug Resistance.
APA
Lambrecht, Ellen, Van Meervenne, E., Boon, N., Van de Wiele, T., Wattiau, P., Herman, L., Heyndrickx, M., et al. (2017). Characterization of cefotaxime- and ciprofloxacin-resistant commensal Escherichia coli originating from Belgian farm animals indicates high antibiotic resistance transfer rates. MICROBIAL DRUG RESISTANCE.
Vancouver
1.
Lambrecht E, Van Meervenne E, Boon N, Van de Wiele T, Wattiau P, Herman L, et al. Characterization of cefotaxime- and ciprofloxacin-resistant commensal Escherichia coli originating from Belgian farm animals indicates high antibiotic resistance transfer rates. MICROBIAL DRUG RESISTANCE. 2017;
MLA
Lambrecht, Ellen, Eva Van Meervenne, Nico Boon, et al. “Characterization of Cefotaxime- and Ciprofloxacin-resistant Commensal Escherichia Coli Originating from Belgian Farm Animals Indicates High Antibiotic Resistance Transfer Rates.” MICROBIAL DRUG RESISTANCE (2017): n. pag. Print.