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Single tracheal inoculation of Aspergillus fumigatus conidia induced aspergillosis in juvenile falcons (Falco spp.)

Dominik Fischer, Lieven Van Waeyenberghe UGent, Klaus Failing, An Martel UGent and Michael Lierz (2017) AVIAN PATHOLOGY. 47(1). p.33-46
abstract
Aspergillosis is a common and life-threatening respiratory disease in raptors with acute and chronic courses. Among raptors, gyrfalcons (Falco rusticolus) and their hybrids are often declared to be highly susceptible with juvenile individuals being the most susceptible. However, species-and age-specific experimental studies are lacking and minimal infective doses (IDs) for Aspergillus spp. conidia are unknown. Therefore, 8-week-old, healthy gyr-hybrid falcons (F. rusticolus X F. cherrug) (N = 18) were experimentally infected with Aspergillus fumigatus using a single intratracheal inoculation with varying dosages of conidia (10(2) to 10(7) conidia). Over 28 days, clinical signs were monitored as well as haematological and serological parameters. Following euthanasia, necropsy, histopathology, bacteriology, and mycology were performed. Re-isolated fungi were compared to the inoculum using microsatellite length polymorphisms. During the trial, clinical signs and dyspnoea correlated significantly with the ID. Necropsy revealed fungal lesions in the upper and lower airways of 10/18 inoculated falcons, but not in the control birds. In 9/18 inoculated falcons, fungal granulomas were confirmed in histopathology and A. fumigatus was re-isolated from these granulomas. Except one nasal isolate all re-isolated fungal strains were identical to the inoculum strain. Based on mycology and histopathology a minimal ID of 50% was calculated to be MID50% (+/- S.E.) = 10(4.52 +/- 0.67) for a single tracheal inoculation of A. fumigatus conidia. This study demonstrates for the first time that a single exposure is able to cause acute aspergillosis in juvenile falcons.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Birds of prey, raptors, mycosis, fungal disease, respiratory tract disease, airway infection, microbiology, falconry, PULMONARY ASPERGILLOSIS, PATHOLOGICAL FINDINGS, COTURNIX-JAPONICA, CLINICAL SIGNS, JAPANESE-QUAIL, TURKEY POULTS, SAUDI-ARABIA, NEW-ZEALAND, BIRDS, INFECTIONS
journal title
AVIAN PATHOLOGY
Avian Pathol.
volume
47
issue
1
pages
33 - 46
Web of Science type
Article
Web of Science id
000424876500005
ISSN
0307-9457
1465-3338
DOI
10.1080/03079457.2017.1360470
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8560058
handle
http://hdl.handle.net/1854/LU-8560058
date created
2018-04-26 12:53:51
date last changed
2018-05-16 09:53:45
@article{8560058,
  abstract     = {Aspergillosis is a common and life-threatening respiratory disease in raptors with acute and chronic courses. Among raptors, gyrfalcons (Falco rusticolus) and their hybrids are often declared to be highly susceptible with juvenile individuals being the most susceptible. However, species-and age-specific experimental studies are lacking and minimal infective doses (IDs) for Aspergillus spp. conidia are unknown. Therefore, 8-week-old, healthy gyr-hybrid falcons (F. rusticolus X F. cherrug) (N = 18) were experimentally infected with Aspergillus fumigatus using a single intratracheal inoculation with varying dosages of conidia (10(2) to 10(7) conidia). Over 28 days, clinical signs were monitored as well as haematological and serological parameters. Following euthanasia, necropsy, histopathology, bacteriology, and mycology were performed. Re-isolated fungi were compared to the inoculum using microsatellite length polymorphisms. During the trial, clinical signs and dyspnoea correlated significantly with the ID. Necropsy revealed fungal lesions in the upper and lower airways of 10/18 inoculated falcons, but not in the control birds. In 9/18 inoculated falcons, fungal granulomas were confirmed in histopathology and A. fumigatus was re-isolated from these granulomas. Except one nasal isolate all re-isolated fungal strains were identical to the inoculum strain. Based on mycology and histopathology a minimal ID of 50\% was calculated to be MID50\% (+/- S.E.) = 10(4.52 +/- 0.67) for a single tracheal inoculation of A. fumigatus conidia. This study demonstrates for the first time that a single exposure is able to cause acute aspergillosis in juvenile falcons.},
  author       = {Fischer, Dominik and Van Waeyenberghe, Lieven and Failing, Klaus and Martel, An and Lierz, Michael},
  issn         = {0307-9457},
  journal      = {AVIAN PATHOLOGY},
  keyword      = {Birds of prey,raptors,mycosis,fungal disease,respiratory tract disease,airway infection,microbiology,falconry,PULMONARY ASPERGILLOSIS,PATHOLOGICAL FINDINGS,COTURNIX-JAPONICA,CLINICAL SIGNS,JAPANESE-QUAIL,TURKEY POULTS,SAUDI-ARABIA,NEW-ZEALAND,BIRDS,INFECTIONS},
  language     = {eng},
  number       = {1},
  pages        = {33--46},
  title        = {Single tracheal inoculation of Aspergillus fumigatus conidia induced aspergillosis in juvenile falcons (Falco spp.)},
  url          = {http://dx.doi.org/10.1080/03079457.2017.1360470},
  volume       = {47},
  year         = {2017},
}

Chicago
Fischer, Dominik, Lieven Van Waeyenberghe, Klaus Failing, An Martel, and Michael Lierz. 2017. “Single Tracheal Inoculation of Aspergillus Fumigatus Conidia Induced Aspergillosis in Juvenile Falcons (Falco Spp.).” Avian Pathology 47 (1): 33–46.
APA
Fischer, Dominik, Van Waeyenberghe, L., Failing, K., Martel, A., & Lierz, M. (2017). Single tracheal inoculation of Aspergillus fumigatus conidia induced aspergillosis in juvenile falcons (Falco spp.). AVIAN PATHOLOGY, 47(1), 33–46.
Vancouver
1.
Fischer D, Van Waeyenberghe L, Failing K, Martel A, Lierz M. Single tracheal inoculation of Aspergillus fumigatus conidia induced aspergillosis in juvenile falcons (Falco spp.). AVIAN PATHOLOGY. 2017;47(1):33–46.
MLA
Fischer, Dominik, Lieven Van Waeyenberghe, Klaus Failing, et al. “Single Tracheal Inoculation of Aspergillus Fumigatus Conidia Induced Aspergillosis in Juvenile Falcons (Falco Spp.).” AVIAN PATHOLOGY 47.1 (2017): 33–46. Print.