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Microbial degradation of zearalenone by Actinobacteria : mind the toxicity

Laura De Mets (UGent) , Kris Audenaert (UGent) , Marthe De Boevre (UGent) , Arnau Vidal Corominas (UGent) , Filip Van Nieuwerburgh (UGent) and Leen De Gelder (UGent)
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Abstract
Mycotoxins are a worldwide problem that continues to challenge the whole food chain. An integrated crop protection system is needed to sufficiently manage fungal and mycotoxin contamination. Microbial and enzyme-based detoxification of contaminated food and feed is a promising bioremediation technique and has many advantages over the use of chemical treatments or physical binding agents. Actinobacteria are known to display a wide catabolic versatility for degradation of several environmental aromatic pollutants, including structurally related mycotoxins. In this research, a collection of Streptomyces sp. and Rhodococcus sp. is screened for the detoxification of zearalenone (ZEN), a non-steroidal estrogen harbouring a benzene ring. Degradation experiments were carried out in rich growth medium containing 5 mg/L ZEN during 72 hours. Microbial degradation was monitored through HPLC-FLD and confirmed through LC-MS/MS, whereas microbial detoxification is monitored through the BLYES assay which determines the estrogenic properties of possible metabolites. Screening of the Actinobacteria showed many strains capable of degrading ZEN, with degradation up to 91,20 % under the used experimental conditions. Remarkably, these high degradation rates coincided with both a significant decrease (-97,96%) and increase (+42,4%) in estrogenic properties of the resulting culture medium, highlighting the importance of toxicity monitoring in the screening process. Through a poly-omics approach combined with toxicity screening, we hope to unravel the degradation pathway of ZEN by the most performant strains and to depict which steps are crucial for detoxification.

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Chicago
De Mets, Laura, Kris Audenaert, Marthe De Boevre, Arnau Vidal Corominas, Filip Van Nieuwerburgh, and Leen De Gelder. 2018. “Microbial Degradation of Zearalenone by Actinobacteria : Mind the Toxicity.” In European Fusarium Seminar, 14th, Abstracts.
APA
De Mets, L., Audenaert, K., De Boevre, M., Vidal Corominas, A., Van Nieuwerburgh, F., & De Gelder, L. (2018). Microbial degradation of zearalenone by Actinobacteria : mind the toxicity. European Fusarium seminar, 14th, Abstracts. Presented at the 14th European Fusarium seminar (EFS 2018).
Vancouver
1.
De Mets L, Audenaert K, De Boevre M, Vidal Corominas A, Van Nieuwerburgh F, De Gelder L. Microbial degradation of zearalenone by Actinobacteria : mind the toxicity. European Fusarium seminar, 14th, Abstracts. 2018.
MLA
De Mets, Laura, Kris Audenaert, Marthe De Boevre, et al. “Microbial Degradation of Zearalenone by Actinobacteria : Mind the Toxicity.” European Fusarium Seminar, 14th, Abstracts. 2018. Print.
@inproceedings{8560031,
  abstract     = {Mycotoxins are a worldwide problem that continues to challenge the whole food chain. An integrated crop protection system is needed to sufficiently manage fungal and mycotoxin contamination. Microbial and enzyme-based detoxification of contaminated food and feed is a promising bioremediation technique and has many advantages over the use of chemical treatments or physical binding agents. Actinobacteria are known to display a wide catabolic versatility for degradation of several environmental aromatic pollutants, including structurally related mycotoxins. In this research, a collection of Streptomyces sp. and Rhodococcus sp. is screened for the detoxification of zearalenone (ZEN), a non-steroidal estrogen harbouring a benzene ring.
Degradation experiments were carried out in rich growth medium containing 5 mg/L ZEN during 72 hours. Microbial degradation was monitored through HPLC-FLD and confirmed through LC-MS/MS, whereas microbial detoxification is monitored through the BLYES assay which determines the estrogenic properties of possible metabolites.
Screening of the Actinobacteria showed many strains capable of degrading ZEN, with degradation up to 91,20 \% under the used experimental conditions. Remarkably, these high degradation rates coincided with both a significant decrease (-97,96\%) and increase (+42,4\%) in estrogenic properties of the resulting culture medium, highlighting the importance of toxicity monitoring in the screening process. Through a poly-omics approach combined with toxicity screening, we hope to unravel the degradation pathway of ZEN by the most performant strains and to depict which steps are crucial for detoxification.},
  author       = {De Mets, Laura and Audenaert, Kris and De Boevre, Marthe and Vidal Corominas, Arnau and Van Nieuwerburgh, Filip and De Gelder, Leen},
  booktitle    = {European Fusarium seminar, 14th, Abstracts},
  language     = {eng},
  location     = {Tulln an der Donau},
  title        = {Microbial degradation of zearalenone by Actinobacteria : mind the toxicity},
  year         = {2018},
}