Advanced search
1 file | 401.66 KB

Presence of Helicobacter and Campylobacter species in faecal samples from zoo mammals

(2018) VETERINARY MICROBIOLOGY. 219. p.49-52
Author
Organization
Abstract
Helicobacter and Campylobacter species (spp.) colonize the gastrointestinal tract of various domesticated animals. Apart from their pathogenic significance in animals, several species are of zoonotic importance as well. For most non-domesticated animal spp., however, little is known on the presence and importance of these agents. Therefore, we investigated the presence of Helicobacter and Campylobacter spp. in marine and terrestrial zoo mammals. Faecal samples of various marine and terrestrial zoo mammals were collected from 6 different zoos in Belgium. These samples were tested for the presence of Helicobacter and Campylobacter spp. by isolation and direct demonstration of DNA using genus-specific PCRs, followed by sequencing of the obtained amplicons. Helicobacter spp. were detected in 12 and Campylobacter spp. in 5 of the 22 faecal samples from marine mammals. In 4 of 5 dolphins, H. cetorum was demonstrated, a well-known pathogen associated with gastritis and gastric ulceration in marine mammals. C. insulaenigrae was isolated from 4 of 6 sea lions and from 1 of 11 seals. To our knowledge, this is the first description of the presence of C. insulaenigrae on the European mainland. Helicobacter spp. were detected in 5 and Campylobacter spp. (mainly C. jejuni subsp. jejuni and C. coli) in 9 of the 26 faecal samples from terrestrial mammals. Potential novel enterohepatic Helicobacter spp. were detected in both marine and terrestrial zoo mammals. For the first time, the presence of several known and unknown Campylobacter and Helicobacter spp. was demonstrated in the gastrointestinal tract of various marine and terrestrial zoo mammals. Further investigation is needed on the pathogenic and zoonotic importance of these species.
Keywords
Helicobacter sp., Campylobacter sp., Zoo mammals, Prevalence, 16S rRNA, GASTRIC HELICOBACTERS, INSULAENIGRAE, PRIMATES, SPP., WILD

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 401.66 KB

Citation

Please use this url to cite or link to this publication:

Chicago
De Witte, Chloë, Celine Lemmens, Bram Flahou, Piet De Laender, Tim Bouts, Francis Vercammen, Richard Ducatelle, Annemieke Smet, and Freddy Haesebrouck. 2018. “Presence of Helicobacter and Campylobacter Species in Faecal Samples from Zoo Mammals.” Veterinary Microbiology 219: 49–52.
APA
De Witte, C., Lemmens, C., Flahou, B., De Laender, P., Bouts, T., Vercammen, F., Ducatelle, R., et al. (2018). Presence of Helicobacter and Campylobacter species in faecal samples from zoo mammals. VETERINARY MICROBIOLOGY, 219, 49–52.
Vancouver
1.
De Witte C, Lemmens C, Flahou B, De Laender P, Bouts T, Vercammen F, et al. Presence of Helicobacter and Campylobacter species in faecal samples from zoo mammals. VETERINARY MICROBIOLOGY. 2018;219:49–52.
MLA
De Witte, Chloë, Celine Lemmens, Bram Flahou, et al. “Presence of Helicobacter and Campylobacter Species in Faecal Samples from Zoo Mammals.” VETERINARY MICROBIOLOGY 219 (2018): 49–52. Print.
@article{8559892,
  abstract     = {Helicobacter and Campylobacter species (spp.) colonize the gastrointestinal tract of various domesticated animals. Apart from their pathogenic significance in animals, several species are of zoonotic importance as well. For most non-domesticated animal spp., however, little is known on the presence and importance of these agents. Therefore, we investigated the presence of Helicobacter and Campylobacter spp. in marine and terrestrial zoo mammals. 
Faecal samples of various marine and terrestrial zoo mammals were collected from 6 different zoos in Belgium. These samples were tested for the presence of Helicobacter and Campylobacter spp. by isolation and direct demonstration of DNA using genus-specific PCRs, followed by sequencing of the obtained amplicons. 
Helicobacter spp. were detected in 12 and Campylobacter spp. in 5 of the 22 faecal samples from marine mammals. In 4 of 5 dolphins, H. cetorum was demonstrated, a well-known pathogen associated with gastritis and gastric ulceration in marine mammals. C. insulaenigrae was isolated from 4 of 6 sea lions and from 1 of 11 seals. To our knowledge, this is the first description of the presence of C. insulaenigrae on the European mainland. Helicobacter spp. were detected in 5 and Campylobacter spp. (mainly C. jejuni subsp. jejuni and C. coli) in 9 of the 26 faecal samples from terrestrial mammals. Potential novel enterohepatic Helicobacter spp. were detected in both marine and terrestrial zoo mammals. 
For the first time, the presence of several known and unknown Campylobacter and Helicobacter spp. was demonstrated in the gastrointestinal tract of various marine and terrestrial zoo mammals. Further investigation is needed on the pathogenic and zoonotic importance of these species.},
  author       = {De Witte, Chlo{\"e} and Lemmens, Celine and Flahou, Bram and De Laender, Piet and Bouts, Tim and Vercammen, Francis and Ducatelle, Richard and Smet, Annemieke and Haesebrouck, Freddy},
  issn         = {0378-1135},
  journal      = {VETERINARY MICROBIOLOGY},
  keyword      = {Helicobacter sp.,Campylobacter sp.,Zoo mammals,Prevalence,16S rRNA,GASTRIC HELICOBACTERS,INSULAENIGRAE,PRIMATES,SPP.,WILD},
  language     = {eng},
  pages        = {49--52},
  title        = {Presence of Helicobacter and Campylobacter species in faecal samples from zoo mammals},
  url          = {http://dx.doi.org/10.1016/j.vetmic.2018.04.014},
  volume       = {219},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: