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Discovery of urinary biomarkers to discriminate between exogenous and semi-endogenous thiouracil in cattle : a parallel-like randomized design

(2018) PLOS ONE. 13(4).
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Abstract
In the European Union, the use of thyreostats for animal fattening purposes has been banned and monitoring plans have been established to detect potential abuse. However, this is not always straightforward as thyreostats such as thiouracil may also have a semi-endogenous origin. Therefore, this study aimed at defining urinary metabolites, which may aid in defining the origin of detected thiouracil. Hereto, a parallel-like randomized in vivo study was conducted in which calves (n = 8) and cows (n = 8) were subjected to either a control treatment, rapeseed-enriched diet to induce semi-endogenous formation, or thiouracil treatment. Urine samples (n = 330) were assessed through metabolic fingerprinting, employing liquid chromatography and Q-Exactive (TM) Orbitrap mass spectrometry. Urinary fingerprints comprised up to 40,000 features whereby multivariate discriminant analysis was able to point out significant metabolome differences between treatments (Q(2)(Y) >= 0.873). Using the validated models, a total of twelve metabolites (including thiouracil) were assigned marker potential. Combining these markers into age-dependent biomarker panels rendered a tool by which sample classification could be improved in comparison with thiouracil-based thresholds, and this during on-going thiouracil treatment (specificities >= 95.2% and sensitivities >= 85.7%), post-treatment (sensitivities >= 80% for >= 24 h after last administration), and simulated low-dose thiouracil treatment (exogenous thiouracil below 30 ng mu L-1). Moreover, the metabolic relevance of revealed markers was supported by the suggested identities, for which a structural link with thiouracil could be determined in most cases. The proposed biomarker panels may contribute to a more justified decision-making in monitoring thiouracil abuse.
Keywords
CHROMATOGRAPHY-MASS-SPECTROMETRY, LIVESTOCK, VALIDATION, SPECTRA, IDENTIFICATION, MODELS

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Chicago
Van Meulebroek, Lieven, Jella Wauters, Beata Pomian, Julie Vanden Bussche, Philippe Delahaut, Eric Fichant, and Lynn Vanhaecke. 2018. “Discovery of Urinary Biomarkers to Discriminate Between Exogenous and Semi-endogenous Thiouracil in Cattle : a Parallel-like Randomized Design.” Plos One 13 (4).
APA
Van Meulebroek, L., Wauters, J., Pomian, B., Vanden Bussche, J., Delahaut, P., Fichant, E., & Vanhaecke, L. (2018). Discovery of urinary biomarkers to discriminate between exogenous and semi-endogenous thiouracil in cattle : a parallel-like randomized design. PLOS ONE, 13(4).
Vancouver
1.
Van Meulebroek L, Wauters J, Pomian B, Vanden Bussche J, Delahaut P, Fichant E, et al. Discovery of urinary biomarkers to discriminate between exogenous and semi-endogenous thiouracil in cattle : a parallel-like randomized design. PLOS ONE. 2018;13(4).
MLA
Van Meulebroek, Lieven, Jella Wauters, Beata Pomian, et al. “Discovery of Urinary Biomarkers to Discriminate Between Exogenous and Semi-endogenous Thiouracil in Cattle : a Parallel-like Randomized Design.” PLOS ONE 13.4 (2018): n. pag. Print.
@article{8559596,
  abstract     = {In the European Union, the use of thyreostats for animal fattening purposes has been banned and monitoring plans have been established to detect potential abuse. However, this is not always straightforward as thyreostats such as thiouracil may also have a semi-endogenous origin. Therefore, this study aimed at defining urinary metabolites, which may aid in defining the origin of detected thiouracil. Hereto, a parallel-like randomized in vivo study was conducted in which calves (n = 8) and cows (n = 8) were subjected to either a control treatment, rapeseed-enriched diet to induce semi-endogenous formation, or thiouracil treatment. Urine samples (n = 330) were assessed through metabolic fingerprinting, employing liquid chromatography and Q-Exactive (TM) Orbitrap mass spectrometry. Urinary fingerprints comprised up to 40,000 features whereby multivariate discriminant analysis was able to point out significant metabolome differences between treatments (Q(2)(Y) {\textrangle}= 0.873). Using the validated models, a total of twelve metabolites (including thiouracil) were assigned marker potential. Combining these markers into age-dependent biomarker panels rendered a tool by which sample classification could be improved in comparison with thiouracil-based thresholds, and this during on-going thiouracil treatment (specificities {\textrangle}= 95.2\% and sensitivities {\textrangle}= 85.7\%), post-treatment (sensitivities {\textrangle}= 80\% for {\textrangle}= 24 h after last administration), and simulated low-dose thiouracil treatment (exogenous thiouracil below 30 ng mu L-1). Moreover, the metabolic relevance of revealed markers was supported by the suggested identities, for which a structural link with thiouracil could be determined in most cases. The proposed biomarker panels may contribute to a more justified decision-making in monitoring thiouracil abuse.},
  articleno    = {e0195351},
  author       = {Van Meulebroek, Lieven and Wauters, Jella and Pomian, Beata and Vanden Bussche, Julie and Delahaut, Philippe and Fichant, Eric and Vanhaecke, Lynn},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  language     = {eng},
  number       = {4},
  pages        = {22},
  title        = {Discovery of urinary biomarkers to discriminate between exogenous and semi-endogenous thiouracil in cattle : a parallel-like randomized design},
  url          = {http://dx.doi.org/10.1371/journal.pone.0195351},
  volume       = {13},
  year         = {2018},
}

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