Ghent University Academic Bibliography

Advanced

Comparative analysis of pathogenic and nonpathogenic Fusarium oxysporum populations associated with banana on a farm in Minas Gerais, Brazil

Pauline Deltour UGent, Soraya de Carvalho França, Lisa Heyman UGent, OL Pereira and Monica Höfte UGent (2018) PLANT PATHOLOGY. 67(3). p.707-718
abstract
Fusarium wilt is one of the most devastating diseases on banana. The causal agent, Fusarium oxysporum f. sp. cubense (Foc) is genetically diverse and its origin and virulence are poorly understood. In this study, pathogenic Foc isolates and nonpathogenic F.oxysporum isolates from Minas Gerais in Brazil were compared using EF-1 and IGS sequences. This allowed the examination of the origin and evolutionary potential of Foc in a country outside the region of origin of the banana plant. Two different sequence types were found among Foc isolates. One appeared to be of local origin because it was identical to the sequence type of the largest group of nonpathogenic isolates. To explore if the local' Foc isolates had acquired pathogenicity either independently through coevolution with the host, or through horizontal gene transfer (HGT) of pathogenicity genes from other, probably introduced, Foc isolates, the presence and sequence of putative SIX effector genes were analysed. Homologues of SIX1, SIX3 and SIX8 were found. SIX1 sequences were identical and exclusively found in all pathogenic isolates, while variable ratios of sequences of multicopy gene SIX8 were found among nonpathogenic and different pathogenic isolates. This observation supports the HGT hypothesis. Horizontal transfer of genes between isolates of F.oxysporum has important implications for the development of reliable diagnostic tools and effective control measures. Full genome sequencing is required to confirm HGT and to further unravel the virulence mechanisms of forma specialis cubense.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
evolution, Panama disease, phylogenetics, virulence, F-SP CUBENSE, HORIZONTAL GENE-TRANSFER, WILT, EFFECTOR, SOIL, COLONIZATION, TOMATO, DIVERSITY, AUSTRALIA, VIRULENCE
journal title
PLANT PATHOLOGY
Plant Pathol.
volume
67
issue
3
pages
707 - 718
Web of Science type
Article
Web of Science id
000426653800019
ISSN
0032-0862
1365-3059
DOI
10.1111/ppa.12757
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8558889
handle
http://hdl.handle.net/1854/LU-8558889
date created
2018-04-12 10:55:23
date last changed
2018-05-16 11:26:55
@article{8558889,
  abstract     = {Fusarium wilt is one of the most devastating diseases on banana. The causal agent, Fusarium oxysporum f. sp. cubense (Foc) is genetically diverse and its origin and virulence are poorly understood. In this study, pathogenic Foc isolates and nonpathogenic F.oxysporum isolates from Minas Gerais in Brazil were compared using EF-1 and IGS sequences. This allowed the examination of the origin and evolutionary potential of Foc in a country outside the region of origin of the banana plant. Two different sequence types were found among Foc isolates. One appeared to be of local origin because it was identical to the sequence type of the largest group of nonpathogenic isolates. To explore if the local' Foc isolates had acquired pathogenicity either independently through coevolution with the host, or through horizontal gene transfer (HGT) of pathogenicity genes from other, probably introduced, Foc isolates, the presence and sequence of putative SIX effector genes were analysed. Homologues of SIX1, SIX3 and SIX8 were found. SIX1 sequences were identical and exclusively found in all pathogenic isolates, while variable ratios of sequences of multicopy gene SIX8 were found among nonpathogenic and different pathogenic isolates. This observation supports the HGT hypothesis. Horizontal transfer of genes between isolates of F.oxysporum has important implications for the development of reliable diagnostic tools and effective control measures. Full genome sequencing is required to confirm HGT and to further unravel the virulence mechanisms of forma specialis cubense.},
  author       = {Deltour, Pauline and de Carvalho Fran\c{c}a, Soraya and Heyman, Lisa and Pereira, OL and H{\"o}fte, Monica},
  issn         = {0032-0862},
  journal      = {PLANT PATHOLOGY},
  keyword      = {evolution,Panama disease,phylogenetics,virulence,F-SP CUBENSE,HORIZONTAL GENE-TRANSFER,WILT,EFFECTOR,SOIL,COLONIZATION,TOMATO,DIVERSITY,AUSTRALIA,VIRULENCE},
  language     = {eng},
  number       = {3},
  pages        = {707--718},
  title        = {Comparative analysis of pathogenic and nonpathogenic Fusarium oxysporum populations associated with banana on a farm in Minas Gerais, Brazil},
  url          = {http://dx.doi.org/10.1111/ppa.12757},
  volume       = {67},
  year         = {2018},
}

Chicago
Deltour, Pauline, Soraya de Carvalho França, Lisa Heyman, OL Pereira, and Monica Höfte. 2018. “Comparative Analysis of Pathogenic and Nonpathogenic Fusarium Oxysporum Populations Associated with Banana on a Farm in Minas Gerais, Brazil.” Plant Pathology 67 (3): 707–718.
APA
Deltour, P., de Carvalho França, S., Heyman, L., Pereira, O., & Höfte, M. (2018). Comparative analysis of pathogenic and nonpathogenic Fusarium oxysporum populations associated with banana on a farm in Minas Gerais, Brazil. PLANT PATHOLOGY, 67(3), 707–718.
Vancouver
1.
Deltour P, de Carvalho França S, Heyman L, Pereira O, Höfte M. Comparative analysis of pathogenic and nonpathogenic Fusarium oxysporum populations associated with banana on a farm in Minas Gerais, Brazil. PLANT PATHOLOGY. 2018;67(3):707–18.
MLA
Deltour, Pauline, Soraya de Carvalho França, Lisa Heyman, et al. “Comparative Analysis of Pathogenic and Nonpathogenic Fusarium Oxysporum Populations Associated with Banana on a Farm in Minas Gerais, Brazil.” PLANT PATHOLOGY 67.3 (2018): 707–718. Print.