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In-feed bambermycin medication induces anti-inflammatory effects and prevents parietal cell loss without influencing Helicobacter suis colonization in the stomach of mice

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Abstract
The minimum inhibitory concentration of bambermycin on three porcine Helicobacter suis strains was shown to be 8 mu g/mL. The effect of in-feed medication with this antibiotic on the course of a gastric infection with one of these strains, the host response and the gastric microbiota was determined in mice, as all of these parameters may be involved in gastric pathology. In H. suis infected mice which were not treated with bambermycin, an increased number of infiltrating B-cells, T-cells and macrophages in combination with a Th2 response was demonstrated, as well as a decreased parietal cell mass. Compared to this non-treated, infected group, in H. suis infected mice medicated with bambermycin, gastric H. suis colonization was not altered, but a decreased number of infiltrating T-cells, B-cells and macrophages as well as downregulated expressions of IL-1 beta, IL-8M, IL-10 and IFN-gamma were demonstrated and the parietal cell mass was not affected. In bambermycin treated mice that were not infected with H. suis, the number of infiltrating T-cells and expression of IL-1 beta were lower than in non-infected mice that did not receive bambermycin. Gastric microbiota analysis indicated that the relative abundance of bacteria that might exert unfavorable effects on the host was decreased during bambermycin supplementation. In conclusion, bambermycin did not affect H. suis colonization, but decreased gastric inflammation and inhibited the effects of a H. suis infection on parietal cell loss. Not only direct interaction of H. suis with parietal cells, but also inflammation may play a role in death of these gastric acid producing cells.
Keywords
ANTIMICROBIAL GROWTH PROMOTERS, RECEPTOR KNOCKOUT MICE, CLOSTRIDIUM-PERFRINGENS, PYLORI INFECTION, BROILER-CHICKENS, DIFFERENT AGES, RIBOSOMAL-RNA, EXPRESSION, FLAVOPHOSPHOLIPOL, PERFORMANCE

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Chicago
De Witte, Chloë, Bernard Taminiau, Bram Flahou, Veerle Hautekiet, Georges Daube, Richard Ducatelle, and Freddy Haesebrouck. 2018. “In-feed Bambermycin Medication Induces Anti-inflammatory Effects and Prevents Parietal Cell Loss Without Influencing Helicobacter Suis Colonization in the Stomach of Mice.” Veterinary Research 49.
APA
De Witte, C., Taminiau, B., Flahou, B., Hautekiet, V., Daube, G., Ducatelle, R., & Haesebrouck, F. (2018). In-feed bambermycin medication induces anti-inflammatory effects and prevents parietal cell loss without influencing Helicobacter suis colonization in the stomach of mice. VETERINARY RESEARCH, 49.
Vancouver
1.
De Witte C, Taminiau B, Flahou B, Hautekiet V, Daube G, Ducatelle R, et al. In-feed bambermycin medication induces anti-inflammatory effects and prevents parietal cell loss without influencing Helicobacter suis colonization in the stomach of mice. VETERINARY RESEARCH. 2018;49.
MLA
De Witte, Chloë, Bernard Taminiau, Bram Flahou, et al. “In-feed Bambermycin Medication Induces Anti-inflammatory Effects and Prevents Parietal Cell Loss Without Influencing Helicobacter Suis Colonization in the Stomach of Mice.” VETERINARY RESEARCH 49 (2018): n. pag. Print.
@article{8558785,
  abstract     = {The minimum inhibitory concentration of bambermycin on three porcine Helicobacter suis strains was shown to be 8 mu g/mL. The effect of in-feed medication with this antibiotic on the course of a gastric infection with one of these strains, the host response and the gastric microbiota was determined in mice, as all of these parameters may be involved in gastric pathology. In H. suis infected mice which were not treated with bambermycin, an increased number of infiltrating B-cells, T-cells and macrophages in combination with a Th2 response was demonstrated, as well as a decreased parietal cell mass. Compared to this non-treated, infected group, in H. suis infected mice medicated with bambermycin, gastric H. suis colonization was not altered, but a decreased number of infiltrating T-cells, B-cells and macrophages as well as downregulated expressions of IL-1 beta, IL-8M, IL-10 and IFN-gamma were demonstrated and the parietal cell mass was not affected. In bambermycin treated mice that were not infected with H. suis, the number of infiltrating T-cells and expression of IL-1 beta were lower than in non-infected mice that did not receive bambermycin. Gastric microbiota analysis indicated that the relative abundance of bacteria that might exert unfavorable effects on the host was decreased during bambermycin supplementation. In conclusion, bambermycin did not affect H. suis colonization, but decreased gastric inflammation and inhibited the effects of a H. suis infection on parietal cell loss. Not only direct interaction of H. suis with parietal cells, but also inflammation may play a role in death of these gastric acid producing cells.},
  articleno    = {35},
  author       = {De Witte, Chlo{\"e} and Taminiau, Bernard and Flahou, Bram and Hautekiet, Veerle and Daube, Georges and Ducatelle, Richard and Haesebrouck, Freddy},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {ANTIMICROBIAL GROWTH PROMOTERS,RECEPTOR KNOCKOUT MICE,CLOSTRIDIUM-PERFRINGENS,PYLORI INFECTION,BROILER-CHICKENS,DIFFERENT AGES,RIBOSOMAL-RNA,EXPRESSION,FLAVOPHOSPHOLIPOL,PERFORMANCE},
  language     = {eng},
  pages        = {16},
  title        = {In-feed bambermycin medication induces anti-inflammatory effects and prevents parietal cell loss without influencing Helicobacter suis colonization in the stomach of mice},
  url          = {http://dx.doi.org/10.1186/s13567-018-0530-1},
  volume       = {49},
  year         = {2018},
}

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