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Regulation of Arabidopsis brassinosteroid receptor BRI1 endocytosis and degradation by plant U-box PUB12/PUB13-mediated ubiquitination

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Abstract
Plants largely rely on plasma membrane (PM)-resident receptor-like kinases (RLKs) to sense extracellular and intracellular stimuli and coordinate cell differentiation, growth, and immunity. Several RLKs have been shown to undergo internalization through the endocytic pathway with a poorly understood mechanism. Here, we show that endocytosis and protein abundance of the Arabidopsis brassinosteroid (BR) receptor, BR INSENSITIVE1 (BRI1), are regulated by plant U-box (PUB) E3 ubiquitin ligase PUB12-and PUB13-mediated ubiquitination. BR perception promotes BRI1 ubiquitination and association with PUB12 and PUB13 through phosphorylation at serine 344 residue. Loss of PUB12 and PUB13 results in reduced BRI1 ubiquitination and internalization accompanied with a prolonged BRI1 PM-residence time, indicating that ubiquitination of BRI1 by PUB12 and PUB13 is a key step in BRI1 endocytosis. Our studies provide a molecular link between BRI1 ubiquitination and internalization and reveal a uniquemechanism of E3 ligase-substrate association regulated by phosphorylation.
Keywords
CLATHRIN-MEDIATED ENDOCYTOSIS, PLASMA-MEMBRANE RECEPTOR, ABSCISIC-ACID, DEPENDENT ENDOCYTOSIS, SIGNAL-TRANSDUCTION, INNATE IMMUNITY, REPEAT, PROTEIN, KINASE, COMPLEX, DOMAIN, Arabidopsis, BRI1, ubiquitination, E3 ligase, endocytosis

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Chicago
Zhou, Jinggeng, Derui Liu, Ping Wang, Xiyu Ma, Wenwei Lin, Sixue Cheng, Kiril Mishev, et al. 2018. “Regulation of Arabidopsis Brassinosteroid Receptor BRI1 Endocytosis and Degradation by Plant U-box PUB12/PUB13-mediated Ubiquitination.” Proceedings of the National Academy of Sciences of the United States of America 115 (8): E1906–E1915.
APA
Zhou, Jinggeng, Liu, D., Wang, P., Ma, X., Lin, W., Cheng, S., Mishev, K., et al. (2018). Regulation of Arabidopsis brassinosteroid receptor BRI1 endocytosis and degradation by plant U-box PUB12/PUB13-mediated ubiquitination. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 115(8), E1906–E1915.
Vancouver
1.
Zhou J, Liu D, Wang P, Ma X, Lin W, Cheng S, et al. Regulation of Arabidopsis brassinosteroid receptor BRI1 endocytosis and degradation by plant U-box PUB12/PUB13-mediated ubiquitination. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2018;115(8):E1906–E1915.
MLA
Zhou, Jinggeng, Derui Liu, Ping Wang, et al. “Regulation of Arabidopsis Brassinosteroid Receptor BRI1 Endocytosis and Degradation by Plant U-box PUB12/PUB13-mediated Ubiquitination.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.8 (2018): E1906–E1915. Print.
@article{8558229,
  abstract     = {Plants largely rely on plasma membrane (PM)-resident receptor-like kinases (RLKs) to sense extracellular and intracellular stimuli and coordinate cell differentiation, growth, and immunity. Several RLKs have been shown to undergo internalization through the endocytic pathway with a poorly understood mechanism. Here, we show that endocytosis and protein abundance of the Arabidopsis brassinosteroid (BR) receptor, BR INSENSITIVE1 (BRI1), are regulated by plant U-box (PUB) E3 ubiquitin ligase PUB12-and PUB13-mediated ubiquitination. BR perception promotes BRI1 ubiquitination and association with PUB12 and PUB13 through phosphorylation at serine 344 residue. Loss of PUB12 and PUB13 results in reduced BRI1 ubiquitination and internalization accompanied with a prolonged BRI1 PM-residence time, indicating that ubiquitination of BRI1 by PUB12 and PUB13 is a key step in BRI1 endocytosis. Our studies provide a molecular link between BRI1 ubiquitination and internalization and reveal a uniquemechanism of E3 ligase-substrate association regulated by phosphorylation.},
  author       = {Zhou, Jinggeng and Liu, Derui and Wang, Ping and Ma, Xiyu and Lin, Wenwei and Cheng, Sixue and Mishev, Kiril and Lu, Dongping and Kumar, Rahul and Vanhoutte, Isabelle and Meng, Xiangzong and He, Ping and Russinova, Eugenia and Shan, Libo},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {CLATHRIN-MEDIATED ENDOCYTOSIS,PLASMA-MEMBRANE RECEPTOR,ABSCISIC-ACID,DEPENDENT ENDOCYTOSIS,SIGNAL-TRANSDUCTION,INNATE IMMUNITY,REPEAT,PROTEIN,KINASE,COMPLEX,DOMAIN,Arabidopsis,BRI1,ubiquitination,E3 ligase,endocytosis},
  language     = {eng},
  number       = {8},
  pages        = {E1906--E1915},
  title        = {Regulation of Arabidopsis brassinosteroid receptor BRI1 endocytosis and degradation by plant U-box PUB12/PUB13-mediated ubiquitination},
  url          = {http://dx.doi.org/10.1073/pnas.1712251115},
  volume       = {115},
  year         = {2018},
}

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