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Long-term in vivo imaging of luciferase-based reporter gene expression in Arabidopsis roots

Wei Xuan (UGent) , Davy Opdenacker (UGent) , Steffen Vanneste (UGent) and Tom Beeckman (UGent)
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Abstract
Plants have an amazing capacity to adjust their growth to environmental limitations. This is particularly relevant for the root system that tunes its developmental pattern to mine the soil for water and nutrients while avoiding patches of soil that contain biotic and abiotic stress agents. Because most developmental processes are taking place gradually while roots are growing, it is often difficult to correlate gene expression events with specific developmental processes that are not necessarily coinciding in time and space. Therefore, in vivo detection and quantification of gene expression over a long period under gravitational conditions can be instrumental in dissecting complex processes in the root. Here, we describe a method for long-term imaging of luciferase dynamics in growing Arabidopsis roots that express a luciferase gene driven by the auxin-output reporter DR5, in the context of lateral root development.
Keywords
Arabidopsis root, DR5 oscillation, Lateral root, Luciferase, Pre-branch site

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Citation

Please use this url to cite or link to this publication:

Chicago
Xuan, Wei, Davy Opdenacker, Steffen Vanneste, and Tom Beeckman. 2018. “Long-term in Vivo Imaging of Luciferase-based Reporter Gene Expression in Arabidopsis Roots.” In Root Development : Methods and Protocols, ed. Daniela Ristova and elke Barbez, 1761:177–190. New York, NY, USA: Springer Humana Press.
APA
Xuan, W., Opdenacker, D., Vanneste, S., & Beeckman, T. (2018). Long-term in vivo imaging of luciferase-based reporter gene expression in Arabidopsis roots. In D. Ristova & elke Barbez (Eds.), Root development : methods and protocols (Vol. 1761, pp. 177–190). New York, NY, USA: Springer Humana Press.
Vancouver
1.
Xuan W, Opdenacker D, Vanneste S, Beeckman T. Long-term in vivo imaging of luciferase-based reporter gene expression in Arabidopsis roots. In: Ristova D, Barbez elke, editors. Root development : methods and protocols. New York, NY, USA: Springer Humana Press; 2018. p. 177–90.
MLA
Xuan, Wei, Davy Opdenacker, Steffen Vanneste, et al. “Long-term in Vivo Imaging of Luciferase-based Reporter Gene Expression in Arabidopsis Roots.” Root Development : Methods and Protocols. Ed. Daniela Ristova & elke Barbez. Vol. 1761. New York, NY, USA: Springer Humana Press, 2018. 177–190. Print.
@incollection{8558186,
  abstract     = {Plants have an amazing capacity to adjust their growth to environmental limitations. This is particularly relevant for the root system that tunes its developmental pattern to mine the soil for water and nutrients while avoiding patches of soil that contain biotic and abiotic stress agents. Because most developmental processes are taking place gradually while roots are growing, it is often difficult to correlate gene expression events with specific developmental processes that are not necessarily coinciding in time and space. Therefore, in vivo detection and quantification of gene expression over a long period under gravitational conditions can be instrumental in dissecting complex processes in the root. Here, we describe a method for long-term imaging of luciferase dynamics in growing Arabidopsis roots that express a luciferase gene driven by the auxin-output reporter DR5, in the context of lateral root development.},
  author       = {Xuan, Wei and Opdenacker, Davy and Vanneste, Steffen and Beeckman, Tom},
  booktitle    = {Root development : methods and protocols},
  editor       = {Ristova, Daniela and Barbez, elke},
  isbn         = {9781493977468},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {177--190},
  publisher    = {Springer Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Long-term in vivo imaging of luciferase-based reporter gene expression in Arabidopsis roots},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7747-5\_13},
  volume       = {1761},
  year         = {2018},
}

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