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Proteome analysis of Arabidopsis roots

Natalia Nikonorova (UGent) , Lam Dai Vu (UGent) , Elisabeth Stes (UGent) , Kris Gevaert (UGent) and Ive De Smet (UGent)
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Abstract
Plant health and development are directly depending on a plant’s ability to react to a constantly changing environment. Sensing of water and nutrition levels and of the biotic environment is vital for a plant, making the root one of the key plant organs. Proteins are the key molecules that play numerous roles in a cell’s everyday life. Quantitative proteome profiling of roots can provide a global overview on the molecular regulatory mechanisms and networks involved in plant growth and development and abiotic and biotic stress responses. Here, we provide a detailed proteomics workflow on Arabidopsis thaliana roots from plant growth up to proteomics data analysis.
Keywords
Arabidopsis thaliana, Label-free proteomics, MaxQuant, Perseus, Root

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Citation

Please use this url to cite or link to this publication:

Chicago
Nikonorova, Natalia, Lam Dai Vu, Elisabeth Stes, Kris Gevaert, and Ive De Smet. 2018. “Proteome Analysis of Arabidopsis Roots.” In Root Development : Methods and Protocols, ed. Daniela Ristova and elke Barbez, 1761:263–274. New York, NY, USA: Springer Humana Press.
APA
Nikonorova, N., Vu, L. D., Stes, E., Gevaert, K., & De Smet, I. (2018). Proteome analysis of Arabidopsis roots. In D. Ristova & elke Barbez (Eds.), Root development : methods and protocols (Vol. 1761, pp. 263–274). New York, NY, USA: Springer Humana Press.
Vancouver
1.
Nikonorova N, Vu LD, Stes E, Gevaert K, De Smet I. Proteome analysis of Arabidopsis roots. In: Ristova D, Barbez elke, editors. Root development : methods and protocols. New York, NY, USA: Springer Humana Press; 2018. p. 263–74.
MLA
Nikonorova, Natalia, Lam Dai Vu, Elisabeth Stes, et al. “Proteome Analysis of Arabidopsis Roots.” Root Development : Methods and Protocols. Ed. Daniela Ristova & elke Barbez. Vol. 1761. New York, NY, USA: Springer Humana Press, 2018. 263–274. Print.
@incollection{8558124,
  abstract     = {Plant health and development are directly depending on a plant{\textquoteright}s ability to react to a constantly changing environment. Sensing of water and nutrition levels and of the biotic environment is vital for a plant, making the root one of the key plant organs. Proteins are the key molecules that play numerous roles in a cell{\textquoteright}s everyday life. Quantitative proteome profiling of roots can provide a global overview on the molecular regulatory mechanisms and networks involved in plant growth and development and abiotic and biotic stress responses. Here, we provide a detailed proteomics workflow on Arabidopsis thaliana roots from plant growth up to proteomics data analysis.},
  author       = {Nikonorova, Natalia and Vu, Lam Dai and Stes, Elisabeth and Gevaert, Kris and De Smet, Ive},
  booktitle    = {Root development : methods and protocols},
  editor       = {Ristova, Daniela and Barbez, elke},
  isbn         = {9781493977468},
  issn         = {1064-3745},
  keyword      = {Arabidopsis thaliana,Label-free proteomics,MaxQuant,Perseus,Root},
  language     = {eng},
  pages        = {263--274},
  publisher    = {Springer Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Proteome analysis of Arabidopsis roots},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7747-5\_20},
  volume       = {1761},
  year         = {2018},
}

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