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Proteome analysis of Arabidopsis roots

Natalia Nikonorova, Lam Dai Vu (UGent) , Elisabeth Stes (UGent) , Kris Gevaert (UGent) and Ive De Smet (UGent)
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Abstract
Plant health and development are directly depending on a plant’s ability to react to a constantly changing environment. Sensing of water and nutrition levels and of the biotic environment is vital for a plant, making the root one of the key plant organs. Proteins are the key molecules that play numerous roles in a cell’s everyday life. Quantitative proteome profiling of roots can provide a global overview on the molecular regulatory mechanisms and networks involved in plant growth and development and abiotic and biotic stress responses. Here, we provide a detailed proteomics workflow on Arabidopsis thaliana roots from plant growth up to proteomics data analysis.
Keywords
Arabidopsis thaliana, Label-free proteomics, MaxQuant, Perseus, Root

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MLA
Nikonorova, Natalia et al. “Proteome Analysis of Arabidopsis Roots.” Root Development : Methods and Protocols. Ed. Daniela Ristova & Elke Barbez. Vol. 1761. New York, NY, USA: Springer Humana Press, 2018. 263–274. Print.
APA
Nikonorova, N., Vu, L. D., Stes, E., Gevaert, K., & De Smet, I. (2018). Proteome analysis of Arabidopsis roots. In D. Ristova & E. Barbez (Eds.), Root development : methods and protocols (Vol. 1761, pp. 263–274). New York, NY, USA: Springer Humana Press.
Chicago author-date
Nikonorova, Natalia, Lam Dai Vu, Elisabeth Stes, Kris Gevaert, and Ive De Smet. 2018. “Proteome Analysis of Arabidopsis Roots.” In Root Development : Methods and Protocols, ed. Daniela Ristova and Elke Barbez, 1761:263–274. New York, NY, USA: Springer Humana Press.
Chicago author-date (all authors)
Nikonorova, Natalia, Lam Dai Vu, Elisabeth Stes, Kris Gevaert, and Ive De Smet. 2018. “Proteome Analysis of Arabidopsis Roots.” In Root Development : Methods and Protocols, ed. Daniela Ristova and Elke Barbez, 1761:263–274. New York, NY, USA: Springer Humana Press.
Vancouver
1.
Nikonorova N, Vu LD, Stes E, Gevaert K, De Smet I. Proteome analysis of Arabidopsis roots. In: Ristova D, Barbez E, editors. Root development : methods and protocols. New York, NY, USA: Springer Humana Press; 2018. p. 263–74.
IEEE
[1]
N. Nikonorova, L. D. Vu, E. Stes, K. Gevaert, and I. De Smet, “Proteome analysis of Arabidopsis roots,” in Root development : methods and protocols, vol. 1761, D. Ristova and E. Barbez, Eds. New York, NY, USA: Springer Humana Press, 2018, pp. 263–274.
@incollection{8558124,
  abstract     = {{Plant health and development are directly depending on a plant’s ability to react to a constantly changing environment. Sensing of water and nutrition levels and of the biotic environment is vital for a plant, making the root one of the key plant organs. Proteins are the key molecules that play numerous roles in a cell’s everyday life. Quantitative proteome profiling of roots can provide a global overview on the molecular regulatory mechanisms and networks involved in plant growth and development and abiotic and biotic stress responses. Here, we provide a detailed proteomics workflow on Arabidopsis thaliana roots from plant growth up to proteomics data analysis.}},
  author       = {{Nikonorova, Natalia and Vu, Lam Dai and Stes, Elisabeth and Gevaert, Kris and De Smet, Ive}},
  booktitle    = {{Root development : methods and protocols}},
  editor       = {{Ristova, Daniela and Barbez, Elke}},
  isbn         = {{9781493977468}},
  issn         = {{1064-3745}},
  keywords     = {{Arabidopsis thaliana,Label-free proteomics,MaxQuant,Perseus,Root}},
  language     = {{eng}},
  pages        = {{263--274}},
  publisher    = {{Springer Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Proteome analysis of Arabidopsis roots}},
  url          = {{http://dx.doi.org/10.1007/978-1-4939-7747-5_20}},
  volume       = {{1761}},
  year         = {{2018}},
}

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