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The function of two type II metacaspases in woody tissues of Populus trees

(2018) NEW PHYTOLOGIST. 217(4). p.1551-1565
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Abstract
Metacaspases (MCs) are cysteine proteases that are implicated in programmed cell death of plants. AtMC9 (Arabidopsis thaliana Metacaspase9) is a member of the Arabidopsis MC family that controls the rapid autolysis of the xylem vessel elements, but its downstream targets in xylem remain uncharacterized. PttMC13 and PttMC14 were identified as AtMC9 homologs in hybrid aspen (Populustremulaxtremuloides). A proteomic analysis was conducted in xylem tissues of transgenic hybrid aspen trees which carried either an overexpression or an RNA interference construct for PttMC13 and PttMC14. The proteomic analysis revealed modulation of levels of both previously known targets of metacaspases, such as Tudor staphylococcal nuclease, heat shock proteins and 14-3-3 proteins, as well as novel proteins, such as homologs of the PUTATIVE ASPARTIC PROTEASE3 (PASPA3) and the cysteine protease RD21 by PttMC13 and PttMC14. We identified here the pathways and processes that are modulated by PttMC13 and PttMC14 in xylem tissues. In particular, the results indicate involvement of PttMC13 and/or PttMC14 in downstream proteolytic processes and cell death of xylem elements. This work provides a valuable reference dataset on xylem-specific metacaspase functions for future functional and biochemical analyses.
Keywords
PROGRAMMED CELL-DEATH, TUDOR STAPHYLOCOCCAL NUCLEASE, ARABIDOPSIS-THALIANA, TRANSCRIPTION FACTORS, PLANT TRANSFORMATION, CYSTEINE PROTEASE, YEAST METACASPASE, STRESS GRANULES, HYBRID ASPEN, EXPRESSION, aspartic protease, cellular autolysis, cysteine protease, metacaspase, Populus, programmed cell death, wood formation, xylem differentiation

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Citation

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MLA
Bollhöner, Benjamin, Soile Jokipii-Lukkari, Joakim Bygdell, et al. “The Function of Two Type II Metacaspases in Woody Tissues of Populus Trees.” NEW PHYTOLOGIST 217.4 (2018): 1551–1565. Print.
APA
Bollhöner, B., Jokipii-Lukkari, S., Bygdell, J., Stael, S., Adriasola, M., Muñiz, L., Van Breusegem, F., et al. (2018). The function of two type II metacaspases in woody tissues of Populus trees. NEW PHYTOLOGIST, 217(4), 1551–1565.
Chicago author-date
Bollhöner, Benjamin, Soile Jokipii-Lukkari, Joakim Bygdell, Simon Stael, Mathilda Adriasola, Luis Muñiz, Frank Van Breusegem, Inés Ezcurra, Gunnar Wingsle, and Hannele Tuominen. 2018. “The Function of Two Type II Metacaspases in Woody Tissues of Populus Trees.” New Phytologist 217 (4): 1551–1565.
Chicago author-date (all authors)
Bollhöner, Benjamin, Soile Jokipii-Lukkari, Joakim Bygdell, Simon Stael, Mathilda Adriasola, Luis Muñiz, Frank Van Breusegem, Inés Ezcurra, Gunnar Wingsle, and Hannele Tuominen. 2018. “The Function of Two Type II Metacaspases in Woody Tissues of Populus Trees.” New Phytologist 217 (4): 1551–1565.
Vancouver
1.
Bollhöner B, Jokipii-Lukkari S, Bygdell J, Stael S, Adriasola M, Muñiz L, et al. The function of two type II metacaspases in woody tissues of Populus trees. NEW PHYTOLOGIST. 2018;217(4):1551–65.
IEEE
[1]
B. Bollhöner et al., “The function of two type II metacaspases in woody tissues of Populus trees,” NEW PHYTOLOGIST, vol. 217, no. 4, pp. 1551–1565, 2018.
@article{8558069,
  abstract     = {Metacaspases (MCs) are cysteine proteases that are implicated in programmed cell death of plants. AtMC9 (Arabidopsis thaliana Metacaspase9) is a member of the Arabidopsis MC family that controls the rapid autolysis of the xylem vessel elements, but its downstream targets in xylem remain uncharacterized. 
PttMC13 and PttMC14 were identified as AtMC9 homologs in hybrid aspen (Populustremulaxtremuloides). A proteomic analysis was conducted in xylem tissues of transgenic hybrid aspen trees which carried either an overexpression or an RNA interference construct for PttMC13 and PttMC14. 
The proteomic analysis revealed modulation of levels of both previously known targets of metacaspases, such as Tudor staphylococcal nuclease, heat shock proteins and 14-3-3 proteins, as well as novel proteins, such as homologs of the PUTATIVE ASPARTIC PROTEASE3 (PASPA3) and the cysteine protease RD21 by PttMC13 and PttMC14. 
We identified here the pathways and processes that are modulated by PttMC13 and PttMC14 in xylem tissues. In particular, the results indicate involvement of PttMC13 and/or PttMC14 in downstream proteolytic processes and cell death of xylem elements. This work provides a valuable reference dataset on xylem-specific metacaspase functions for future functional and biochemical analyses.},
  author       = {Bollhöner, Benjamin and Jokipii-Lukkari, Soile and Bygdell, Joakim and Stael, Simon and Adriasola, Mathilda and Muñiz, Luis and Van Breusegem, Frank and Ezcurra, Inés and Wingsle, Gunnar and Tuominen, Hannele},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  keywords     = {PROGRAMMED CELL-DEATH,TUDOR STAPHYLOCOCCAL NUCLEASE,ARABIDOPSIS-THALIANA,TRANSCRIPTION FACTORS,PLANT TRANSFORMATION,CYSTEINE PROTEASE,YEAST METACASPASE,STRESS GRANULES,HYBRID ASPEN,EXPRESSION,aspartic protease,cellular autolysis,cysteine protease,metacaspase,Populus,programmed cell death,wood formation,xylem differentiation},
  language     = {eng},
  number       = {4},
  pages        = {1551--1565},
  title        = {The function of two type II metacaspases in woody tissues of Populus trees},
  url          = {http://dx.doi.org/10.1111/nph.14945},
  volume       = {217},
  year         = {2018},
}

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