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Development and application of a duplex PCR assay for detection of Crangon crangon bacilliform virus in populations of European brown shrimp (Crangon crangon)

Benigna Van Eynde UGent, Olivier Christiaens UGent, Daan Delbare, Kris Cooreman, Kelly S Bateman, Grant D Stentiford, Annette M Dullemans, Monique M van Oers and Guy Smagghe UGent (2018) JOURNAL OF INVERTEBRATE PATHOLOGY. 153. p.195-202
abstract
Crangon crangon bacilliform virus (CcBV) was first discovered in 2004 in European brown shrimp (Crangon crangon) caught along the English coast. This study describes a duplex PCR assay developed for the detection of CcBV, based on amplification of the lef-8 gene (211 bp) of CcBV and the E75 gene (105 bp) of C. crangon as an internal amplification control. The lef-8 and E75 primer pairs were designed based on preliminary genome sequencing information of the virus and transcriptomic data available for C. crangon, respectively. Sequencing of the resulting amplicons confirmed the specificity of this PCR assay and sequence analysis of the lef-8 fragment revealed amino acid identity percentages ranging between 31 and 42% with members of the Nudiviridae, proposing that CcBV may reside within this family. Finally, the duplex PCR assay was applied to samples of C. crangon hepatopancreas tissue collected along the Belgian coast to screen for the presence of CcBV. The prevalence of CcBV averaged 87%, which is comparable to previous reports of high prevalence, based upon histological analysis, in shrimp collected along the English coast. Development of a specific and sensitive PCR assay to detect CcBV will provide a useful tool for future aquaculture and research programs involving C. crangon.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Crustacean, Disease, CcBV, Nudiviridae, Diagnostic, Aquaculture, SPOT SYNDROME VIRUS, YELLOW-HEAD VIRUS, INTERNAL AMPLIFICATION CONTROLS, POLYMERASE-CHAIN-REACTION, FRESH-WATER CRAYFISH, PENAEID SHRIMP, MONOCLONAL-ANTIBODIES, PARALYSIS-VIRUS, DNA EXTRACTION, NECROSIS VIRUS
journal title
JOURNAL OF INVERTEBRATE PATHOLOGY
J. Invertebr. Pathol.
volume
153
pages
195 - 202
Web of Science type
Article
Web of Science id
000429892400026
ISSN
0022-2011
DOI
10.1016/j.jip.2018.03.006
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8557720
handle
http://hdl.handle.net/1854/LU-8557720
date created
2018-03-28 14:06:46
date last changed
2018-05-16 10:40:44
@article{8557720,
  abstract     = {Crangon crangon bacilliform virus (CcBV) was first discovered in 2004 in European brown shrimp (Crangon crangon) caught along the English coast. This study describes a duplex PCR assay developed for the detection of CcBV, based on amplification of the lef-8 gene (211 bp) of CcBV and the E75 gene (105 bp) of C. crangon as an internal amplification control. The lef-8 and E75 primer pairs were designed based on preliminary genome sequencing information of the virus and transcriptomic data available for C. crangon, respectively. Sequencing of the resulting amplicons confirmed the specificity of this PCR assay and sequence analysis of the lef-8 fragment revealed amino acid identity percentages ranging between 31 and 42\% with members of the Nudiviridae, proposing that CcBV may reside within this family. 
Finally, the duplex PCR assay was applied to samples of C. crangon hepatopancreas tissue collected along the Belgian coast to screen for the presence of CcBV. The prevalence of CcBV averaged 87\%, which is comparable to previous reports of high prevalence, based upon histological analysis, in shrimp collected along the English coast. Development of a specific and sensitive PCR assay to detect CcBV will provide a useful tool for future aquaculture and research programs involving C. crangon.},
  author       = {Van Eynde, Benigna and Christiaens, Olivier and Delbare, Daan and Cooreman, Kris and Bateman, Kelly S and Stentiford, Grant D and Dullemans, Annette M and van Oers, Monique M and Smagghe, Guy},
  issn         = {0022-2011},
  journal      = {JOURNAL OF INVERTEBRATE PATHOLOGY},
  keyword      = {Crustacean,Disease,CcBV,Nudiviridae,Diagnostic,Aquaculture,SPOT SYNDROME VIRUS,YELLOW-HEAD VIRUS,INTERNAL AMPLIFICATION CONTROLS,POLYMERASE-CHAIN-REACTION,FRESH-WATER CRAYFISH,PENAEID SHRIMP,MONOCLONAL-ANTIBODIES,PARALYSIS-VIRUS,DNA EXTRACTION,NECROSIS VIRUS},
  language     = {eng},
  pages        = {195--202},
  title        = {Development and application of a duplex PCR assay for detection of Crangon crangon bacilliform virus in populations of European brown shrimp (Crangon crangon)},
  url          = {http://dx.doi.org/10.1016/j.jip.2018.03.006},
  volume       = {153},
  year         = {2018},
}

Chicago
Van Eynde, Benigna, Olivier Christiaens, Daan Delbare, Kris Cooreman, Kelly S Bateman, Grant D Stentiford, Annette M Dullemans, Monique M van Oers, and Guy Smagghe. 2018. “Development and Application of a Duplex PCR Assay for Detection of Crangon Crangon Bacilliform Virus in Populations of European Brown Shrimp (Crangon Crangon).” Journal of Invertebrate Pathology 153: 195–202.
APA
Van Eynde, B., Christiaens, O., Delbare, D., Cooreman, K., Bateman, K. S., Stentiford, G. D., Dullemans, A. M., et al. (2018). Development and application of a duplex PCR assay for detection of Crangon crangon bacilliform virus in populations of European brown shrimp (Crangon crangon). JOURNAL OF INVERTEBRATE PATHOLOGY, 153, 195–202.
Vancouver
1.
Van Eynde B, Christiaens O, Delbare D, Cooreman K, Bateman KS, Stentiford GD, et al. Development and application of a duplex PCR assay for detection of Crangon crangon bacilliform virus in populations of European brown shrimp (Crangon crangon). JOURNAL OF INVERTEBRATE PATHOLOGY. 2018;153:195–202.
MLA
Van Eynde, Benigna, Olivier Christiaens, Daan Delbare, et al. “Development and Application of a Duplex PCR Assay for Detection of Crangon Crangon Bacilliform Virus in Populations of European Brown Shrimp (Crangon Crangon).” JOURNAL OF INVERTEBRATE PATHOLOGY 153 (2018): 195–202. Print.