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Crustacean cardioactive peptide (CCAP) of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) : molecular characterization, distribution and its potential roles in larva-pupa ecdysis

(2019) PEPTIDES. 122.
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Abstract
Insects must undergo ecdysis for successful development and growth, and the crustacean cardioactive peptide (CCAP) is one of the most important hormone in this process. Here we reported a cDNA encoding for the CCAP precursor cloned from the oriental fruit fly, Bactrocera dorsalis, a most destructive insect pest of agriculture. The CCAP mature peptide (PFCNAFTGC-NH2) of B. dorsalis was generated by post-translational processing and found to be highly comparable with other insects. RT-qPCR showed that mRNA of CCAP in B. dorsalis (BdCCAP) was predominantly expressed in the central nervous system (CNS) and midgut of 3rd-instar larvae. By using immunohistochemical analysis, we also localized the endocrine cells that produce CCAP in the CNS, ring gland and midgut of 3rd-instar larvae of B. dorsalis. The synthetic CCAP mature peptide could induce the expression of mRNA of adipokinetic hormone (AKH), the metabolic neuropeptides in insects. The expression of BdCCAP mRNA in the CNS, but not in the midgut, could be upregulated in the response to the challenge of insect molting hormone, 20-hydroxyecdysone.
Keywords
Crustacean cardioactive peptide, Immunohistochemistry, 20-hydroxyecdysone, Bactrocera dorsalis, Ecdysis, RED FLOUR BEETLE, STOMATOGASTRIC GANGLION, ADIPOKINETIC HORMONE, NERVOUS-SYSTEM, SHORE CRAB, INSECT, BEHAVIOR, LOCUST, IDENTIFICATION, DROSOPHILA

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MLA
Shi, Yan, et al. “Crustacean Cardioactive Peptide (CCAP) of the Oriental Fruit Fly, Bactrocera Dorsalis (Diptera: Tephritidae) : Molecular Characterization, Distribution and Its Potential Roles in Larva-Pupa Ecdysis.” PEPTIDES, vol. 122, 2019.
APA
Shi, Y., Liu, T.-Y., Pei, Y.-X., Jiang, H.-B., Dou, W., Smagghe, G., & Wang, J.-J. (2019). Crustacean cardioactive peptide (CCAP) of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) : molecular characterization, distribution and its potential roles in larva-pupa ecdysis. PEPTIDES, 122.
Chicago author-date
Shi, Yan, Tian-Yuan Liu, Yu-Xia Pei, Hong-Bo Jiang, Wei Dou, Guy Smagghe, and Jin-Jun Wang. 2019. “Crustacean Cardioactive Peptide (CCAP) of the Oriental Fruit Fly, Bactrocera Dorsalis (Diptera: Tephritidae) : Molecular Characterization, Distribution and Its Potential Roles in Larva-Pupa Ecdysis.” PEPTIDES 122.
Chicago author-date (all authors)
Shi, Yan, Tian-Yuan Liu, Yu-Xia Pei, Hong-Bo Jiang, Wei Dou, Guy Smagghe, and Jin-Jun Wang. 2019. “Crustacean Cardioactive Peptide (CCAP) of the Oriental Fruit Fly, Bactrocera Dorsalis (Diptera: Tephritidae) : Molecular Characterization, Distribution and Its Potential Roles in Larva-Pupa Ecdysis.” PEPTIDES 122.
Vancouver
1.
Shi Y, Liu T-Y, Pei Y-X, Jiang H-B, Dou W, Smagghe G, et al. Crustacean cardioactive peptide (CCAP) of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) : molecular characterization, distribution and its potential roles in larva-pupa ecdysis. PEPTIDES. 2019;122.
IEEE
[1]
Y. Shi et al., “Crustacean cardioactive peptide (CCAP) of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) : molecular characterization, distribution and its potential roles in larva-pupa ecdysis,” PEPTIDES, vol. 122, 2019.
@article{8557718,
  abstract     = {Insects must undergo ecdysis for successful development and growth, and the crustacean cardioactive peptide (CCAP) is one of the most important hormone in this process. Here we reported a cDNA encoding for the CCAP precursor cloned from the oriental fruit fly, Bactrocera dorsalis, a most destructive insect pest of agriculture. The CCAP mature peptide (PFCNAFTGC-NH2) of B. dorsalis was generated by post-translational processing and found to be highly comparable with other insects. RT-qPCR showed that mRNA of CCAP in B. dorsalis (BdCCAP) was predominantly expressed in the central nervous system (CNS) and midgut of 3rd-instar larvae. By using immunohistochemical analysis, we also localized the endocrine cells that produce CCAP in the CNS, ring gland and midgut of 3rd-instar larvae of B. dorsalis. The synthetic CCAP mature peptide could induce the expression of mRNA of adipokinetic hormone (AKH), the metabolic neuropeptides in insects. The expression of BdCCAP mRNA in the CNS, but not in the midgut, could be upregulated in the response to the challenge of insect molting hormone, 20-hydroxyecdysone.},
  articleno    = {169929},
  author       = {Shi, Yan and Liu, Tian-Yuan and Pei, Yu-Xia and Jiang, Hong-Bo and Dou, Wei and Smagghe, Guy and Wang, Jin-Jun},
  issn         = {0196-9781},
  journal      = {PEPTIDES},
  keywords     = {Crustacean cardioactive peptide,Immunohistochemistry,20-hydroxyecdysone,Bactrocera dorsalis,Ecdysis,RED FLOUR BEETLE,STOMATOGASTRIC GANGLION,ADIPOKINETIC HORMONE,NERVOUS-SYSTEM,SHORE CRAB,INSECT,BEHAVIOR,LOCUST,IDENTIFICATION,DROSOPHILA},
  language     = {eng},
  pages        = {7},
  title        = {Crustacean cardioactive peptide (CCAP) of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) : molecular characterization, distribution and its potential roles in larva-pupa ecdysis},
  url          = {http://dx.doi.org/10.1016/j.peptides.2018.02.007},
  volume       = {122},
  year         = {2019},
}

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